| FLORIDA CLASSIC CONSORTIUM CORPORATION | Tallahassee | S20 Community Improvement & Capacity Building | $4.8M | 4 |
| THE TREE OF LIFE FOUNDATION INTERNATIONAL INC | West Palm Beach | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $4.8M | 0 |
| CONCEPT HEALTH SYSTEM INC | Doral | F22Z Mental Health & Crisis Intervention | $4.8M | 1 |
| I E C SOCIAL EDUCATIONAL & HUMANITARIAN AID INC | Orlando | B60 Education | $4.8M | 3 |
| FOUNDATION FOR APPLIED MOLECULAR EVOLUTION INC | Alachua | B820 Education | $4.8M | 5 |
| FLORIDA CARPENTERS REGIONAL COUNCIL | Fort Lauderdale | — | $4.8M | 9 |
| PEPIN DISTRIBUTING COMPANY GROUP BENEFIT TRUST | Tampa | — | $4.8M | 1 |
| CHILD PROTECTION CENTER INC | Sarasota | I700 Crime & Legal-Related | $4.8M | 3 |
| BOCA GRANDE HEALTH CLINIC INC | Boca Grande | E32Z Health Care | $4.7M | 4 |
| PELICAN ISLE YACHT CLUB INC | Naples | N50 Recreation & Sports | $4.7M | 3 |
| RENAISSANCE THEATRE COMPANY INC | Orlando | A65 Arts, Culture & Humanities | $4.7M | 4 |
| OCEAN REEF CULTURAL CENTER INC | Key Largo | A200 Arts, Culture & Humanities | $4.7M | 1 |
| EMERALD COAST ASSOCIATION OF REALTORS INC | Fort Walton Beach | — | $4.7M | 2 |
| THE BANNER LAKE CLUB INC | Hobe Sound | O50 Youth Development | $4.7M | 1 |
| UNIVERSITY PREPARATORY ACADEMY PALM BEACH INC | West Palm Beach | B29 Education | $4.7M | 1 |
| THE WALTER J GATTI FOUNDATION INC | Melbourne | H00Z Medical Research | $4.7M | 0 |
| ALACHUA COUNTY COALITION FOR THE HOMELESS AND HUNGRY INC | Gainesville | P85 Human Services | $4.7M | 1 |
| BRADENTON YACHT CLUB INC | Palmetto | — | $4.7M | 2 |
| NORTH BROWARD HOSPITAL DISTRICT CHARITABLE FOUNDATION INC | Fort Lauderdale | E11 Health Care | $4.7M | 1 |
| PALM BEACH GARDENS YOUTH ATHLETIC ASSOCIATIONINC | Palm Beach Gardens | — | $4.7M | 1 |
| EMERALD COAST AUTISM CENTER IN | Niceville | B20 Education | $4.7M | 2 |
| Revived Soldiers Ukraine | Orlando | Q33 International, Foreign Affairs & National Security | $4.7M | 3 |
| FLORIDA ASSOCIATION OF DISTRICT SCHOOL SUPERINTENDENTS INC | Tallahassee | — | $4.7M | 6 |
| Human Rights Defense Center Inc | Lake Worth Beach | Z99Z Unknown | $4.7M | 3 |
| NATHAN BENDERSON PARK CONSERVANCY INC | Sarasota | N32 Recreation & Sports | $4.7M | 0 |
| LIGHTHOUSE OF PINELLAS INC | Largo | — | $4.7M | 1 |
| St Augustine Lighthouse & Maritime Museum | Saint Augustine | A540 Arts, Culture & Humanities | $4.7M | 3 |
| UNITED STATES MASTERS SWIMMING INC | Sarasota | N67Z Recreation & Sports | $4.7M | 7 |
| DAYTONA AREA SENIOR SERVICES INC | Daytona Beach | P20 Human Services | $4.7M | 0 |
| THE CARL C ICAHN FOUNDATION (FKA FNDTN FOR A GREATER OPPORTUNITY) | Sunny Isles Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $4.7M | 0 |
| True School Inc | Clearwater | B24Z Education | $4.7M | 1 |
| UNITED STATES LAW ENFORCEMENT FOUNDATION INC | Titusville | — | $4.7M | 4 |
| St Petersburg Country ClubInc | St Petersburg | — | $4.7M | 2 |
| FBH COMMUNITY INC | Daytona Beach | K31 Food, Agriculture & Nutrition | $4.6M | 0 |
| BEYOND THE SPECTRUM INC | Sarasota | G84 Diseases, Disorders & Medical Disciplines | $4.6M | 1 |
| THE COLLECTION ON PALMETTO FOUNDATION INC | Clearwater | A50 Arts, Culture & Humanities | $4.6M | 0 |
| COUNCIL ON AGING OF ST LUCIE INC | Port St Lucie | P81Z Human Services | $4.6M | 1 |
| WINTER PARK LIBRARY ASSOCIATION INC | Winter Park | B70 Education | $4.6M | 1 |
| THE CENTER OF PROGESS AND EXCELLENCE INC | Fort Myers | F30 Mental Health & Crisis Intervention | $4.6M | 2 |
| EDWARD C FOGG III & LISBETH A FOGG CHARITABLE TRUST | Tallahassee | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $4.6M | 5 |
| Chi Chi Rodriguez Youth Foundation Inc | Clearwater | — | $4.6M | 2 |
| LOCAL UNION 177 OF THE INTERNATIONAL BROTHERHOOD OF ELECTRICAL WORKERS | Jacksonville | — | $4.6M | 9 |
| GRACE CHRISTIAN SCHOOL OF OCALA FLORIDA INC | Ocala | B24 Education | $4.6M | 1 |
| EG Watkins Family Foundation c/o Bessemer Trust | Stuart | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $4.6M | 0 |
| KIDCO CREATIVE LEARNING INC | Hialeah | P33Z Human Services | $4.6M | 1 |
| CITY OF PALMS CHARTER HIGH SCHOOL INC | Fort Myers | B29 Education | $4.6M | 1 |
| MIAMI HOMES FOR ALL INC | Miami | P20 Human Services | $4.6M | 2 |
| NORTH KEY LARGO UTILITY CORP | Key Largo | — | $4.6M | 6 |
| SENIOR CITIZENS HOUSING DEVELOPMENT CORPORATION OF CHICAGO | Plantation | L22 Housing & Shelter | $4.6M | 1 |
| ACADEMIE DA VINCI CHARTER SCHOOL INC | Dunedin | B99Z Education | $4.6M | 1 |
