| EARLY LEARNING COALITION OF FLORIDAS | Port Charlotte | B90 Education | $20.9M | 0 |
| NAVIGATOR ACADEMY OF LEADERSHIP INC | Brandon | — | $20.8M | 0 |
| THE ROCK FOUNDATION | North Palm Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $20.8M | 0 |
| ROYAL PALM YACHT AND COUNTRY CLUB INC | Boca Raton | — | $20.8M | 7 |
| CENTRAL FLORIDA KIDNEY CENTERS INC | Orlando | G44Z Diseases, Disorders & Medical Disciplines | $20.8M | 6 |
| UNIVERSITY OF NORTH FLORIDA FOUNDATION | Jacksonville | B110 Education | $20.7M | 4 |
| FLORIDA GULF COAST UNIVERISTY | Fort Myers | T11 Philanthropy, Voluntarism & Grantmaking Foundations | $20.7M | 2 |
| FAMILY CENTRAL INC | Ft Lauderdale | P330 Human Services | $20.7M | 4 |
| BayCare Alliant Hospital Inc | Dunedin | E24 Health Care | $20.6M | 8 |
| PLACE OF HOPE INC | Palm Beach Gardens | P70 Human Services | $20.6M | 5 |
| BRIDGE PREP ACADEMY OF TAMPA INC | Miami | — | $20.5M | 1 |
| CATHOLIC HEALTH SERVICES INC | Lauderdale Lakes | — | $20.5M | 8 |
| REGENERATIVE BIOLOGICS INC | Gainesville | E65 Health Care | $20.4M | 5 |
| SUPPORT OUR TROOPS INC | Daytona Beach | — | $20.3M | 1 |
| STATE CHARTERED CREDIT UNIONS IN FLORIDA | Pensacola | — | $20.3M | 7 |
| SUNRISE NORTHEAST INC | Miami | L21 Housing & Shelter | $20.2M | 5 |
| Catholic Charities Bureau Inc | Jacksonville | P200 Human Services | $20.2M | 1 |
| DESTIN WATER USERS INC | Destin | — | $20.2M | 4 |
| DANIEL MEMORIAL INC | Jacksonville | F33Z Mental Health & Crisis Intervention | $20.2M | 5 |
| SANTAFE HEALTHCARE INC | Gainesville | E60 Health Care | $20.2M | 20 |
| FAMILY FIRST INC | Tampa | P010 Human Services | $20.2M | 8 |
| THE CLUB PELICAN BAY INC | Naples | — | $20.2M | 9 |
| EARLY LEARNING COALITION OF | Shalimar | B90 Education | $20.1M | 4 |
| PENNEY RETIREMENT COMMUNITY INC | Penney Farms | P750 Human Services | $20.1M | 4 |
| CONSOLIDATED CREDIT SOLUTIONS INC | Fort Lauderdale | — | $20M | 8 |
| OVERLAND MISSIONS INC | Cocoa | P99 Human Services | $20M | 2 |
| West Florida Health Home Care Inc | Temple Terrace | E11 Health Care | $20M | 14 |
| TOM FORD FAMILY FOUNDATION | Palm Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $20M | 0 |
| BIG BEND CARES INC | Tallahassee | — | $20M | 2 |
| ANN STORCK CENTER INC | Fort Lauderdale | — | $20M | 1 |
| CITRUS RESEARCH AND DEVELOPMENT | Lake Alfred | — | $20M | 1 |
| Orange Bowl Committee Inc | Miami Lakes | B42 Education | $19.9M | 7 |
| ASSISTANCE UNLIMITED INC | Fort Lauderdale | P33 Human Services | $19.8M | 0 |
| PERSONAL ENRICHMENT THROUGH MENTAL | Pinellas Park | — | $19.8M | 10 |
| The Preservation Foundation of Palm | Palm Beach | A82Z Arts, Culture & Humanities | $19.8M | 1 |
| HOSPICE OF MARTIN & ST LUCIE INC | Stuart | P74Z Human Services | $19.8M | 9 |
| VNA Hospice of Indian River County Inc | Vero Beach | — | $19.7M | 2 |
| The Scully Peretsman Foundation | Miami Beach | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $19.7M | 0 |
| INDIVIDUAL EDUCATION PLAN ACADEMY INC | Kissimmee | B20 Education | $19.7M | 2 |
| WILLIAM MONROE ROWLETT ACADEMY FOR ARTS | Bradenton | B20 Education | $19.6M | 3 |
| CHILDREN'S FORUM INC | Tallahassee | P330 Human Services | $19.6M | 7 |
| CENTER FOR THE ADVANCEMENT OF | Rockledge | U05 Science & Technology | $19.5M | 9 |
| THE LANGHAM PRIVATE FOUNDATION TRUST | Sarasota | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $19.4M | 1 |
| MATER ACADEMY FOUNDATION | Miami | B11 Education | $19.4M | 0 |
| MIAMI BEACH FRATERNAL ORDER OF | Miami Beach | — | $19.4M | 0 |
| THE MCGREGOR CLINIC INC | Fort Myers | E30 Health Care | $19.3M | 6 |
| SAWGRASS COUNTRY CLUB INC | Ponte Vedra Bch | N50 Recreation & Sports | $19.3M | 6 |
| GUARDIANS CREDIT UNION | West Palm Beach | — | $19.3M | 7 |
| THE SUNDARI FOUNDATION INC | Miami Beach | L41 Housing & Shelter | $19.2M | 11 |
| WESTMINSTER SHORES INC | Orlando | P75Z Human Services | $19.2M | 7 |
