| BRIDGE PREP ACADEMY OF TAMPA INC | Miami | — | $20.5M | 1 |
| CAREERSOURCE FLORIDA INC | Tallahassee | J20 Employment | $20.5M | 9 |
| CATHOLIC HEALTH SERVICES INC | Lauderdale Lakes | — | $20.5M | 8 |
| LEHRMAN COMMUNITY DAY SCHOOL INC | Miami Beach | B24 Education | $20.5M | 8 |
| MIAMI ASSOCIATION OF REALTORS INC | Miami | — | $20.4M | 9 |
| SUPPORT OUR TROOPS INC | Daytona Beach | — | $20.3M | 1 |
| Episcopal Day Private School of Manatee County Inc | Bradenton | B200 Education | $20.3M | 8 |
| SUNRISE NORTHEAST INC | Miami | L21 Housing & Shelter | $20.2M | 5 |
| STATE CHARTERED CREDIT UNIONS IN FLORIDA LOYALTY CREDIT UNION | Pensacola | — | $20.2M | 11 |
| HOSPICE OF MARTIN & ST LUCIE INC | Stuart | P74Z Human Services | $20.2M | 9 |
| Catholic Charities Bureau Inc | Jacksonville | P200 Human Services | $20.2M | 1 |
| DESTIN WATER USERS INC | Destin | — | $20.2M | 4 |
| Museum of Science and History of Jacksonville Inc | Jacksonville | A500 Arts, Culture & Humanities | $20.2M | 1 |
| SANTAFE HEALTHCARE INC | Gainesville | E60 Health Care | $20.2M | 20 |
| THE CLUB PELICAN BAY INC | Naples | — | $20.2M | 9 |
| EARLY LEARNING COALITION OF OKALOOSA & WALTON COUNTIES INC | Shalimar | B90 Education | $20.1M | 4 |
| PENNEY RETIREMENT COMMUNITY INC | Penney Farms | P750 Human Services | $20.1M | 4 |
| CONCORDIA OF FLORIDA INC | Tampa | L22 Housing & Shelter | $20.1M | 9 |
| Florida Foundation Seed Producers Inc | Greenwood | K05 Food, Agriculture & Nutrition | $20.1M | 1 |
| OVERLAND MISSIONS INC | Cocoa | P99 Human Services | $20M | 2 |
| EARLY LEARNING COALITION OF THE NATURE COAST INC | Crystal River | B192 Education | $20M | 2 |
| CENTRAL FLORIDA KIDNEY CENTERS INC | Orlando | G44Z Diseases, Disorders & Medical Disciplines | $20M | 6 |
| West Florida Health Home Care Inc | Temple Terrace | E11 Health Care | $20M | 14 |
| BIG BEND CARES INC | Tallahassee | — | $20M | 2 |
| ANN STORCK CENTER INC | Fort Lauderdale | — | $20M | 1 |
| PANHANDLE CREDIT UNION | Panama City | — | $20M | 9 |
| CITRUS RESEARCH AND DEVELOPMENT FOUNDATION INC | Lake Alfred | — | $20M | 1 |
| JEWISH COMMUNITY CENTERS OF SOUTH BROWARD INC | Davie | P280 Human Services | $19.9M | 6 |
| ASSISTANCE UNLIMITED INC | Fort Lauderdale | P33 Human Services | $19.8M | 0 |
| The Preservation Foundation of Palm Beach Inc | Palm Beach | A82Z Arts, Culture & Humanities | $19.8M | 1 |
| THE WARREN AND AUGUSTA HUME FOUNDATION INC | Ponte Vedra Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $19.8M | 7 |
| VNA Hospice of Indian River County Inc | Vero Beach | — | $19.7M | 2 |
| OAK HALL PRIVATE SCHOOL INC | Gainesville | — | $19.7M | 7 |
| The Scully Peretsman Foundation | Miami Beach | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $19.7M | 0 |
| INDIVIDUAL EDUCATION PLAN ACADEMY INC | Kissimmee | B20 Education | $19.7M | 2 |
| AGAPE NETWORK INC | Miami | I43Z Crime & Legal-Related | $19.7M | 7 |
| NATIONAL GIFT ANNUITY FOUNDATION | Jacksonville | T21Z Philanthropy, Voluntarism & Grantmaking Foundations | $19.6M | 0 |
| WILLIAM MONROE ROWLETT ACADEMY FOR ARTS AND COMMUNICATION | Bradenton | B20 Education | $19.6M | 3 |
| CENTER FOR THE ADVANCEMENT OF SCIENCE IN SPACE INC | Rockledge | U05 Science & Technology | $19.5M | 9 |
| MASON CLASSICAL ACADEMY INC | Naples | B29 Education | $19.4M | 2 |
| MIAMI BEACH FRATERNAL ORDER OF POLICE HEALTH TRUST | Miami Beach | — | $19.4M | 0 |
| BRAVO FAMILY FOUNDATION | Miami | Q33 International, Foreign Affairs & National Security | $19.3M | 3 |
| THE MCGREGOR CLINIC INC | Fort Myers | E30 Health Care | $19.3M | 6 |
| SAWGRASS COUNTRY CLUB INC | Ponte Vedra Bch | N50 Recreation & Sports | $19.3M | 6 |
| THE SUNDARI FOUNDATION INC | Miami Beach | L41 Housing & Shelter | $19.2M | 11 |
| Bruce and Deborah Duncan Foundation | Naples | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $19.2M | 0 |
| WESTMINSTER SHORES INC | Orlando | P75Z Human Services | $19.2M | 7 |
| SALVADOR DALI MUSEUM INC | St Petersburg | A510 Arts, Culture & Humanities | $19.2M | 4 |
| STOOPS FAMILY FOUNDATION | Delray Beach | T99 Philanthropy, Voluntarism & Grantmaking Foundations | $19.2M | 0 |
| DISC VILLAGE INC | Tallahassee | F200 Mental Health & Crisis Intervention | $19.2M | 6 |
