| INTERNATIONAL BROTHERHOOD OF ELECTRICAL WORKERS LOCAL 55 | Des Moines | J404 Employment | $3.5M | 15 |
| DENTAL CONNECTIONS INC | Des Moines | E300 Health Care | $3.5M | 4 |
| FARMERS ELECTRIC COOPERATIVE | Kalona | — | $3.5M | 6 |
| Athene Charitable Foundation | West Des Moines | T99 Philanthropy, Voluntarism & Grantmaking Foundations | $3.5M | 0 |
| NEIGHBORHOOD CENTERS OF JOHNSON COUNTY | Iowa City | P28Z Human Services | $3.5M | 2 |
| Power Co-op Employees Credit Union | Humboldt | — | $3.5M | 1 |
| TREES FOREVER INC | Marion | C30Z Environment | $3.5M | 5 |
| Holthues Trust | Muscatine | T90Z Philanthropy, Voluntarism & Grantmaking Foundations | $3.5M | 0 |
| NATIONAL CZECH & SLOVAK MUSEUM & LIBRARY INC | Cedar Rapids | A500 Arts, Culture & Humanities | $3.5M | 1 |
| OPPORTUNITY HOMES INC | Decorah | P73Z Human Services | $3.5M | 1 |
| METCO CREDIT UNION | Cedar Rapids | — | $3.5M | 1 |
| WILLIS DADY EMERGENCY SHELTER INC | Cedar Rapids | — | $3.5M | 1 |
| POLK COUNTY HOUSING TRUST FUND | Des Moines | L20 Housing & Shelter | $3.5M | 1 |
| UNITED ASSOCIATION OF JOURNEYMEN 125 PPF | Cedar Rapids | — | $3.5M | 5 |
| UNITED FOOD AND COMMERCIAL WORKERS INTERNATIONAL UNION NO 431 | Davenport | — | $3.5M | 22 |
| WAYLAND MENNONITE HOME | Wayland | P74Z Human Services | $3.4M | 1 |
| GREATER GREENFIELD COMMUNITY FOUNDATION | Greenfield | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $3.4M | 1 |
| Maharishi Integrated Health Foundation | Fairfield | E11 Health Care | $3.4M | 1 |
| ACCESS INC | Hampton | J300 Employment | $3.4M | 1 |
| LANSING HOUSING CORPORATION | Lansing | — | $3.4M | 1 |
| RURAL WATER SYSTEM NUMBER 1 | Hospers | — | $3.4M | 11 |
| BARTHELL OES HOME | Decorah | E910 Health Care | $3.4M | 2 |
| SUCCESSFUL LIVING SUPPORTIVE HOUSING PROGRAM | Iowa City | L80 Housing & Shelter | $3.4M | 1 |
| UNITED WAYS OF IOWA | Johnston | S20 Community Improvement & Capacity Building | $3.4M | 1 |
| AVENUE SCHOLARS IOWA | Council Bluffs | B90 Education | $3.4M | 4 |
| STACYVILLE COMMUNITY NURSING HOME | Stacyville | E91Z Health Care | $3.4M | 0 |
| BOYS & GIRLS CLUBS OF THE CORRIDOR | Cedar Rapids | N31 Recreation & Sports | $3.4M | 1 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF WASHINGTON IOWA | Washington | P270 Human Services | $3.4M | 1 |
| TRAILHEAD INTERNATIONAL BUILDERS INC | Waukee | Q33 International, Foreign Affairs & National Security | $3.4M | 2 |
| MEALS FROM THE HEARTLAND INC | West Des Moines | K30 Food, Agriculture & Nutrition | $3.4M | 1 |
| UNITED WAY AND COMMUNITY FOUNDATION OF GREATER FORT DODGE | Fort Dodge | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $3.4M | 1 |
| THE ART CENTER ASSOCIATION OF SIOUX CITY | Sioux City | — | $3.4M | 0 |
| DOWNTOWN PARKING MANAGEMENT INC | Cedar Rapids | S99 Community Improvement & Capacity Building | $3.4M | 0 |
| TRINITY HEALTH SYSTEMS INC | Fort Dodge | E210 Health Care | $3.4M | 11 |
| RONALD MCDONALD HOUSE CHARITIES OF EASTERN IOWA AND WESTERN ILLINOIS | Iowa City | E86Z Health Care | $3.4M | 1 |
| EASTWOOD OF AMES INC | Spencer | L990 Housing & Shelter | $3.4M | 2 |
| TIMELY MISSION NURSING HOME INC | Buffalo Center | E91Z Health Care | $3.4M | 0 |
| IOWA BEEF CATTLE PRODUCERS ASSOCIATION DBA IOWA BEEF INDUSTRY COUNCIL | Ames | — | $3.4M | 1 |
| Marie H Bechtel Charitable Trust | Davenport | T90 Philanthropy, Voluntarism & Grantmaking Foundations | $3.3M | 1 |
| Underground Location Company dba Iowa One Call | Des Moines | — | $3.3M | 0 |
| Iowa Agricultural Mitigation Inc | Burlington | C34 Environment | $3.3M | 0 |
| IOWA STATE BAR ASSOCIATION | Des Moines | — | $3.3M | 2 |
| DYERSVILLE INDUSTRIES INC | Dyersville | S300 Community Improvement & Capacity Building | $3.3M | 0 |
| Berkshire Hathaway Energy Foundation | Des Moines | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $3.3M | 0 |
| THE ARC OF EAST CENTRAL IOWA | Cedar Rapids | B99 Education | $3.3M | 2 |
| JASPER COMMUNITY FOUNDATION | Newton | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $3.2M | 0 |
| DOWNTOWN DES MOINES SELF-SUPPORTED MUNICIPAL IMPROVEMENT DISTRICT | Des Moines | S41 Community Improvement & Capacity Building | $3.2M | 0 |
| COOPERATIVE TELEPHONE COMPANY | Victor | — | $3.2M | 8 |
| CENTER AT SIXTH | Des Moines | S31 Community Improvement & Capacity Building | $3.2M | 0 |
| MAHASKA RURAL WATER SYSTEMS INC | Oskaloosa | — | $3.2M | 8 |
