| Wells Fargo & Co Employee Benefit Trust | Des Moines | — | $2.7B | 0 |
| PRAIRIE MEADOWS RACE TRACK AND CASINO | Altoona | — | $2.5B | 9 |
| CENTRAL IOWA HOSPITAL CORPORATION | Des Moines | E220 Health Care | $1.2B | 32 |
| CATHOLIC HEALTH INITIATIVES - IOWA CORP | Des Moines | E220 Health Care | $896.6M | 15 |
| MERCY HEALTH SERVICES - IOWA CORP | Mason City | E210 Health Care | $881.2M | 19 |
| IOWA PHYSICIANS CLINIC MEDICAL | West Des Moines | E300 Health Care | $612.2M | 52 |
| GreenState Credit Union | North Liberty | — | $577.6M | 22 |
| Genesis Health System | Davenport | E20 Health Care | $566.6M | 13 |
| IOWA HEALTH SYSTEM | West Des Moines | E02 Health Care | $495.1M | 53 |
| ST LUKE'S METHODIST HOSPITAL | Cedar Rapids | E220 Health Care | $477.1M | 15 |
| VERIDIAN CREDIT UNION | Waterloo | W61 Public & Societal Benefit | $459.8M | 22 |
| SOUTHEAST IOWA REGIONAL MEDICAL | West Burlington | E220 Health Care | $438.2M | 8 |
| MERCY MEDICAL CENTER | Cedar Rapids | E220 Health Care | $435.7M | 12 |
| ALLEN MEMORIAL HOSPITAL CORPORATION | Waterloo | E220 Health Care | $371.7M | 15 |
| THE TRUSTEES OF GRINNELL COLLEGE | Grinnell | B420 Education | $364.4M | 18 |
| INTERMEDIARYED | Mount Vernon | B900 Education | $352.5M | 26 |
| COVENANT MEDICAL CENTER INC | Waterloo | E220 Health Care | $328.8M | 15 |
| UNITYPOINT AT HOME | West Des Moines | — | $271.9M | 23 |
| DRAKE UNIVERSITY | Des Moines | B430 Education | $263.1M | 10 |
| CARE INITIATIVES | West Des Moines | E910 Health Care | $261.1M | 19 |
| DELTA DENTAL OF IOWA | Johnston | — | $246.5M | 24 |
| NORTHWEST IOWA HOSPITAL CORPORATION | Sioux City | E220 Health Care | $221.3M | 15 |
| CENTRAL IOWA POWER COOPERATIVE | Cedar Rapids | — | $217.8M | 27 |
| HY-VEE AND AFFILIATES' BENEFIT PLAN | West Des Moines | — | $213.2M | 0 |
| MERCY CLINICS INC | Des Moines | E30Z Health Care | $193.5M | 15 |
| Dupaco Community Credit Union | Dubuque | — | $186.5M | 14 |
| TRINITY REGIONAL MEDICAL CENTER | Fort Dodge | E220 Health Care | $184.1M | 12 |
| CORN BELT POWER COOPERATIVE | Humboldt | — | $172.6M | 20 |
| JENNIE EDMUNDSON MEMORIAL HOSPITAL | Council Bluffs | E220 Health Care | $172.2M | 10 |
| STATE UNIVERSITY OF IOWA FOUNDATION | Iowa City | B110 Education | $167.6M | 11 |
| IOWA BANKERS BENEFIT PLAN | Johnston | — | $166.6M | 1 |
| LINCOLN NATIONAL CORPORATION VEBA TRUST | Des Moines | — | $166.2M | 1 |
| Mercy Hospital Iowa City Iowa | Iowa City | E220 Health Care | $164.5M | 11 |
| COMMUNITY FDN OF GREATER DES MOINES | Des Moines | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $160.8M | 8 |
| PELLA REGIONAL HEALTH CENTER | Pella | E220 Health Care | $148.5M | 12 |
| IOWA STATE UNIVERSITY FOUNDATION | Ames | B110 Education | $147M | 11 |
| THE FINLEY HOSPITAL | Dubuque | E220 Health Care | $146.7M | 11 |
| LUTHER COLLEGE | Decorah | B420 Education | $132.2M | 12 |
| MERCY MEDICAL CENTER - CLINTON INC | Clinton | E220 Health Care | $127.2M | 13 |
| Wells Fargo & Co Short Term Disability Plan & Trust | Des Moines | Y43 Mutual & Membership Benefit | $122M | 0 |
| ST AMBROSE UNIVERSITY | Davenport | B430 Education | $120.9M | 12 |
| NORTHWEST IOWA POWER COOPERATIVE | Le Mars | — | $114.6M | 15 |
| WARTBURG COLLEGE | Waverly | B420 Education | $108.6M | 10 |
| DES MOINES UNIVERSITY OSTEOPATHIC | West Des Moines | B500 Education | $108.2M | 12 |
| WESLEY RETIREMENT SERVICES INC | Johnston | P750 Human Services | $107M | 10 |
| UNIVERSITY OF DUBUQUE | Dubuque | B430 Education | $104M | 7 |
| IOWA STUDENT LOAN LIQUIDITY | West Des Moines | B820 Education | $101.5M | 22 |
| ERIC AND KELLY SCHWARTZ CHARITABLE TRUST | Fairfield | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $99.7M | 2 |
| CITY OF RALEIGH NC MEDICAL AND DENTAL BENFIT | Des Moines | — | $98.6M | 1 |
| St Anthony Regional Hospital | Carroll | E220 Health Care | $94.4M | 9 |
