| The Nebraska Medical Center | Omaha | E210 Health Care | $2.3B | 23 |
| THE SUSAN THOMPSON BUFFETT FOUNDATION | Omaha | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $1.7B | 2 |
| WOODMEN OF THE WORLD LIFE INSURANCE SOCIETY | Omaha | — | $1.2B | 41 |
| Bryan Medical Center | Lincoln | E220 Health Care | $917M | 18 |
| ALEGENT HEALTH - BERGAN MERCY HEALTH SYSTEM | Omaha | E210 Health Care | $792.7M | 14 |
| CHILDREN'S HOSPITAL & MEDICAL CENTER | Omaha | E240 Health Care | $774.5M | 9 |
| WALTER SCOTT FAMILY FOUNDATION | Omaha | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $762.5M | 3 |
| Creighton University | Omaha | B430 Education | $750.2M | 16 |
| NEBRASKA METHODIST HOSPITAL | Omaha | E220 Health Care | $743.7M | 14 |
| THE SHERWOOD FOUNDATION | Omaha | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $653.1M | 2 |
| ALEGENT CREIGHTON CLINIC | Omaha | E30 Health Care | $580.6M | 26 |
| UNMC Physicians | Omaha | E220 Health Care | $564.5M | 18 |
| Father Flanagan's Boys' Home | Boys Town | P730 Human Services | $503.4M | 13 |
| ALEGENT CREIGHTON HEALTH | Omaha | E210 Health Care | $480.4M | 21 |
| Eagle Foundation | Omaha | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $447M | 0 |
| MOSAIC | Omaha | L99 Housing & Shelter | $374.2M | 27 |
| UNIVERSITY OF NEBRASKA FOUNDATION | Lincoln | B110 Education | $352.5M | 9 |
| SUZANNE & WALTER SCOTT FOUNDATION | Omaha | T900 Philanthropy, Voluntarism & Grantmaking Foundations | $327.9M | 11 |
| PHYSICIANS CLINIC INC | Omaha | E320 Health Care | $306.6M | 13 |
| NORTH PLATTE NEBRASKA HOSPITAL CORP | North Platte | E220 Health Care | $295.7M | 9 |
| FAITH REGIONAL HEALTH SERVICES | Norfolk | E220 Health Care | $275.3M | 9 |
| Nebraska Electric Generation & Transmission Cooperative Inc | Columbus | — | $256M | 21 |
| VETTER SENIOR LIVING | Elkhorn | E91 Health Care | $248.8M | 8 |
| ALEGENT HEALTH-IMMANUEL MEDICAL CENTER | Omaha | E220 Health Care | $230.6M | 17 |
| REGIONAL WEST MEDICAL CENTER | Scottsbluff | E220 Health Care | $230.4M | 14 |
| THE MARY LANNING MEMORIAL HOSPITAL ASSOCIATION | Hastings | E220 Health Care | $222.8M | 8 |
| GOOD SAMARITAN HOSPITAL | Kearney | E220 Health Care | $205.1M | 14 |
| SAINT ELIZABETH REGIONAL MEDICAL CENTER | Lincoln | E220 Health Care | $192M | 18 |
| MADONNA REHABILITATION HOSPITAL | Lincoln | E240 Health Care | $189M | 17 |
| COLUMBUS COMMUNITY HOSPITAL INC | Columbus | E220 Health Care | $175.3M | 12 |
| Bryan Hospital Kearney | Kearney | E22 Health Care | $172.6M | 12 |
| METHODIST FREMONT HEALTH | Omaha | E22 Health Care | $170.4M | 11 |
| FILKINS FAMILY FOUNDATION | Omaha | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $162.6M | 0 |
| Bellevue University | Bellevue | B430 Education | $160.1M | 14 |
| SAINT FRANCIS MEDICAL CENTER | Grand Island | E220 Health Care | $141.9M | 17 |
| Nebraska Medicine | Omaha | E21 Health Care | $141.5M | 4 |
| Omaha Community Foundation | Omaha | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $124.8M | 7 |
| PHELPS MEMORIAL HEALTH CENTER | Holdrege | E220 Health Care | $116.9M | 15 |
| NEBRASKA PEDIATRIC PRACTICE INC | Omaha | E240 Health Care | $112.4M | 12 |
| NATIONAL ARBOR DAY FOUNDATION | Lincoln | C500 Environment | $111.9M | 17 |
| THE HAWKS FOUNDATION | Omaha | B82 Education | $108.5M | 1 |
| Peter Kiewit Foundation | Omaha | T70I Philanthropy, Voluntarism & Grantmaking Foundations | $105.1M | 7 |
| METROPOLITAN ENTERTAINMENT & CONVENTION AUTHORITY | Omaha | S30 Community Improvement & Capacity Building | $101.6M | 6 |
| BEATRICE COMMUNITY HOSPITAL & HEALTH CENTER INC | Beatrice | E220 Health Care | $101.3M | 11 |
| Bryan Physician Network | Lincoln | E300 Health Care | $97.9M | 12 |
| Grand Island Regional Medical Center Inc | Grand Island | E20 Health Care | $86.8M | 11 |
| NEBRASKA METHODIST HEALTH SYSTEM INC | Omaha | E210 Health Care | $86.4M | 18 |
| CHEYENNE COUNTY HOSPITAL ASSOCIATION INC | Sidney | E220 Health Care | $85.6M | 8 |
| NEBRASKA WESLEYAN UNIVERSITY | Lincoln | B420 Education | $84.9M | 13 |
| Nebraska Bankers Assn Voluntary Employees Beneficiary Association | Lincoln | — | $82.7M | 0 |
