| SJC CULTURAL EVENTS INC | St Augustine | A60 Arts, Culture & Humanities | $43.6M | 2 |
| Baptist Health System Foundation Inc | Jacksonville | — | $43.4M | 7 |
| THE BENJAMIN PRIVATE SCHOOL INC | North Palm Beach | B20Z Education | $43.4M | 7 |
| CLOVERFIELDS FOUNDATION | Naples | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $43.3M | 0 |
| THE KOVNER FOUNDATION | Jupiter | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $43.1M | 0 |
| TIM TEBOW FOUNDATION INC | Jacksonville | T50 Philanthropy, Voluntarism & Grantmaking Foundations | $43.1M | 5 |
| Hospice by the Sea Inc | Lakewood Ranch | — | $42.9M | 0 |
| BAYCARE PASCO INC | Wesley Chapel | E20 Health Care | $42.9M | 8 |
| Worthington Scholarship Foundation | Naples | T23 Philanthropy, Voluntarism & Grantmaking Foundations | $42.9M | 1 |
| OAK HAMMOCK AT THE UNIVERSITY OF | Gainesville | P750 Human Services | $42.5M | 7 |
| Senior Connection Center Inc | Tampa | P81I Human Services | $42.4M | 4 |
| Chuck and Marna Davis Foundation | Key Largo | — | $42.4M | 0 |
| UNITED WAY OF NORTHEAST FLORIDA INC | Jacksonville | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $42.4M | 8 |
| BREVARD ACHIEVEMENT CENTER INC | Rockledge | J33Z Employment | $42.2M | 5 |
| Boca Raton Regional Hospital Foundation Inc | Boca Raton | S21 Community Improvement & Capacity Building | $42.2M | 1 |
| Jessie Trice Community Health System Inc | Miami | E320 Health Care | $41.9M | 14 |
| METROPOLITAN MINISTRIES INC | Tampa | P850 Human Services | $41.9M | 8 |
| YMCA OF SOUTHWEST FLORIDA INC | Venice | — | $41.8M | 10 |
| MID FLORIDA COMMUNITY SERVICES INC | Brooksville | P200 Human Services | $41.7M | 5 |
| DR HERBERT AND NICOLE WERTHEIM FAMILY | Miami | — | $41.7M | 0 |
| Communities Connected for Kids Inc | Port St Lucie | P30 Human Services | $41.6M | 5 |
| GOODWILL INDUSTRIES - BIG BEND INC | Tallahassee | — | $41.4M | 7 |
| FLORIDA NETWORK OF YOUTH AND FAMILY | Tallahassee | P300 Human Services | $41.4M | 4 |
| THE LATIN ACADEMY OF RECORDING | Miami | — | $41.3M | 12 |
| JORGE M PEREZ ART MUSEUM OF MIAMI-DADE | Miami | A510 Arts, Culture & Humanities | $41.3M | 6 |
| JOSEPH L MORSE HEALTH CENTER INC | West Palm Beach | E91 Health Care | $41.3M | 9 |
| St Petersburg Free Clinic Inc | St Petersburg | — | $41.3M | 5 |
| United Home Care Services Inc | Miami | E92Z Health Care | $41.3M | 11 |
| ORLAND BETHEL FAMILY FOUNDATION | Port St Lucie | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $41.1M | 0 |
| BALLENISLES COUNTRY CLUB INC | Palm Beach Gardens | N50 Recreation & Sports | $41.1M | 7 |
| OSCEOLA CONVENTION AND VISITORS BUREAU INC | Kissimmee | S41 Community Improvement & Capacity Building | $40.7M | 5 |
| PLATO ACADEMY SCHOOLS CORPORATION | Clearwater | B99 Education | $40.6M | 0 |
| The Florida Aquarium Inc | Tampa | D50 Animal-Related | $40.6M | 9 |
| THE COUNTRY CLUB AT WOODFIELD INC | Boca Raton | N6A Recreation & Sports | $40.5M | 8 |
| CARON OF FLORIDA INC | Delray Beach | F220 Mental Health & Crisis Intervention | $40.5M | 10 |
| RAYMOND F KRAVIS CENTER | West Palm Beach | A610 Arts, Culture & Humanities | $40.5M | 11 |
| ESCAMBIA SCHOOL DISTRICT BENEFIT TRUST | Pensacola | — | $40.3M | 0 |
| TrustBridge Inc | Lakewood Ranch | P11 Human Services | $40.3M | 0 |
| NORTH FLORIDA RETIREMENT VILLAGE INC | Gainesville | P750 Human Services | $40.1M | 7 |
| THE EVERGLADES CLUB INC | Palm Beach | — | $40M | 9 |
| VILLA MARIA NURSING & | North Miami | E50 Health Care | $39.8M | 10 |
| SOUTH ATLANTIC ILA EMPLOYERS | Jacksonville | — | $39.7M | 0 |
| THE INSTITUTE FOR INTERGOVERNMENTAL | Tallahassee | L20 Housing & Shelter | $39.7M | 17 |
| BAY HAVEN CHARTER ACADEMY INC | Panama City | B302 Education | $39.6M | 2 |
| TWIN OAKS JUVENILE DEVELOPMENT INC | Tallahassee | I44 Crime & Legal-Related | $39.6M | 6 |
| JEWISH COMMUNITY SERVICES OF SOUTH | Miami | P40 Human Services | $39.5M | 6 |
| ARNIE'S ARMY CHARITABLE FOUNDATION INC | Orlando | P11 Human Services | $39.4M | 1 |
| THE SEMINOLE COUNTY COALITION FOR | Longwood | B112 Education | $39.4M | 1 |
| CAMILLUS HOUSE INC | Miami | — | $39.3M | 6 |
| JACKSONVILLE ZOOLOGICAL SOCIETY INC | Jacksonville | D500 Animal-Related | $39.3M | 7 |
