| Early Learning Coalition of Pasco and Hernando Counties Inc | Hudson | B12 Education | $46.1M | 4 |
| HEART OF FLORIDA HEALTH CENTER INC | Ocala | E32Z Health Care | $45.8M | 14 |
| UNITED CEREBRAL PALSY OF CENTRAL FLORIDA INC | Orlando | G23Z Diseases, Disorders & Medical Disciplines | $45.7M | 3 |
| RAYMOND F KRAVIS CENTER FOR THE PERFORMING ARTS INC | West Palm Beach | A610 Arts, Culture & Humanities | $45.4M | 11 |
| Charles & Margery Barancik Foundation Inc | Sarasota | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $45.4M | 3 |
| UNIVERSITY OF FLORIDA RESEARCH FOUNDATION INC | Gainesville | B110 Education | $45.3M | 23 |
| Morton Plant Mease Health Care Inc | Clearwater | E210 Health Care | $45.2M | 11 |
| Metropolitan Charities Inc | St Petersburg | P800 Human Services | $45M | 9 |
| EMPATH HEALTH INC | Lakewood Ranch | P117 Human Services | $44.8M | 14 |
| LOVE A CHILD INC | Fort Myers | Q330 International, Foreign Affairs & National Security | $44.5M | 5 |
| Florida Memorial University Inc | Miami Gardens | B420 Education | $44.5M | 0 |
| ATLANTA PLUMBERS & STEAMFITTERS | Pembroke Pines | — | $44.4M | 0 |
| The Society of the Four Arts Inc | Palm Beach | A200 Arts, Culture & Humanities | $44.3M | 9 |
| STATE CHARTERED CREDIT UNIONS IN FLORIDA POWER FINANCIAL CREDIT UNION | Pembroke Pines | — | $44.2M | 16 |
| Habitat for Humanity of Collier County Inc | Naples | L200 Housing & Shelter | $44M | 4 |
| Cox Science Center and Aquarium Inc | West Palm Beach | A570 Arts, Culture & Humanities | $43.9M | 2 |
| GMP-EMPLOYERS RETIREE TRUST | Fort Myers | — | $43.8M | 1 |
| H LEE MOFFITT CANCER CENTER & RESEARCH INSTITUTE FOUNDATION INC | Tampa | G110 Diseases, Disorders & Medical Disciplines | $43.8M | 11 |
| AMATEUR ATHLETIC UNION OF THE US INC C/O WALT DISNEY RESORTS | Lake Buena Vista | — | $43.8M | 14 |
| THE AFFORDABLE HOUSING PRESERVATION CORPORATION | Jacksonville | L21 Housing & Shelter | $43.6M | 1 |
| SJC CULTURAL EVENTS INC | St Augustine | A60 Arts, Culture & Humanities | $43.6M | 2 |
| KIPP JACKSONVILLE INC | Jacksonville | B24 Education | $43.5M | 2 |
| THE RENWICK FOUNDATION - USA | Longboat Key | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $43.5M | 0 |
| MIAMI DADE COLLEGE FOUNDATION INC | Miami | B110 Education | $43.4M | 5 |
| Baptist Health System Foundation Inc | Jacksonville | — | $43.4M | 7 |
| TIM TEBOW FOUNDATION INC | Jacksonville | T50 Philanthropy, Voluntarism & Grantmaking Foundations | $43.1M | 5 |
| Hospice by the Sea Inc | Lakewood Ranch | — | $42.9M | 0 |
| BAYCARE PASCO INC | Wesley Chapel | E20 Health Care | $42.9M | 8 |
| Worthington Scholarship Foundation | Naples | T23 Philanthropy, Voluntarism & Grantmaking Foundations | $42.9M | 1 |
| FLORIDA STATE UNIVERSITY ATHLETICS ASSOCIATION INC | Tallahassee | N11 Recreation & Sports | $42.9M | 7 |
| THE SCHOOLS OF MCKEEL ACADEMY INC | Lakeland | B20 Education | $42.4M | 5 |
| Senior Connection Center Inc | Tampa | P81I Human Services | $42.4M | 4 |
| Chuck and Marna Davis Foundation FKA Charles A & Marna Davis Foundation | Key Largo | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $42.4M | 0 |
| BREVARD ACHIEVEMENT CENTER INC | Rockledge | J33Z Employment | $42.2M | 5 |
| Boca Raton Regional Hospital Foundation Inc | Boca Raton | S21 Community Improvement & Capacity Building | $42.2M | 1 |
| TWIN OAKS JUVENILE DEVELOPMENT INC | Tallahassee | I44 Crime & Legal-Related | $42.1M | 6 |
| United Home Care Services Inc | Miami | E92Z Health Care | $42M | 11 |
| Florida West Coast Symphony Inc dba Sarasota Orchestra | Sarasota | A690 Arts, Culture & Humanities | $42M | 3 |
| GOODWILL INDUSTRIES - BIG BEND INC | Tallahassee | — | $41.9M | 7 |
| METROPOLITAN MINISTRIES INC | Tampa | P850 Human Services | $41.9M | 8 |
| YMCA OF SOUTHWEST FLORIDA INC | Venice | — | $41.8M | 10 |
| MID FLORIDA COMMUNITY SERVICES INC | Brooksville | P200 Human Services | $41.7M | 5 |
| DR HERBERT AND NICOLE WERTHEIM FAMILY FOUNDATION INC | Miami | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $41.7M | 0 |
| ARNIE'S ARMY CHARITABLE FOUNDATION INC | Orlando | P11 Human Services | $41.6M | 1 |
| Communities Connected for Kids Inc | Port St Lucie | P30 Human Services | $41.6M | 5 |
| BALLENISLES COUNTRY CLUB INC | Palm Beach Gardens | N50 Recreation & Sports | $41.5M | 8 |
| JORGE M PEREZ ART MUSEUM OF MIAMI-DADE COUNTY INC | Miami | A510 Arts, Culture & Humanities | $41.3M | 6 |
| JOSEPH L MORSE HEALTH CENTER INC | West Palm Beach | E91 Health Care | $41.3M | 9 |
| St Petersburg Free Clinic Inc | St Petersburg | — | $41.3M | 5 |
| ORLAND BETHEL FAMILY FOUNDATION | Port St Lucie | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $41.1M | 0 |
