| Hernando Pasco Hospice Inc | Temple Terrace | P74Z Human Services | $49.8M | 16 |
| YOUTH CO-OP INC | Miami | J200 Employment | $49.7M | 5 |
| AdventHealth Home Health and Hospice | Maitland | P74Z Human Services | $49.7M | 8 |
| Hospice of Marion County Inc | Lakewood Ranch | P74 Human Services | $49.5M | 9 |
| JEWISH FEDERATION OF PALM BEACH COUNTY | West Palm Beach | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $49.4M | 9 |
| BayCare Behavioral Health Inc | New Port Richey | F200 Mental Health & Crisis Intervention | $48.7M | 11 |
| Lowry Park Zoological Society of Tampa | Tampa | D500 Animal-Related | $48.6M | 10 |
| Best Buddies International Inc | Miami | Q20 International, Foreign Affairs & National Security | $48.3M | 7 |
| WATERMAN COMMUNITIES INC | Mount Dora | P750 Human Services | $48.2M | 8 |
| FLORIDA COMMUNITY | West Palm Beach | — | $48.1M | 6 |
| AMERICAN MARITIME OFFICERS MEDICAL PLAN | Dania Beach | — | $47.8M | 17 |
| CENTRAL FLORIDA REGIONAL WORKFORCE | Orlando | J22 Employment | $47.8M | 12 |
| EARLY LEARNING COALITION OF OSCEOLA | Kissimmee | B99 Education | $47.5M | 4 |
| MENTAL HEALTH RESOURCE CENTER INC | Jacksonville | E99Z Health Care | $47.2M | 7 |
| LIFE CARE PONTE VEDRA INC | Ponte Vedra Beach | P750 Human Services | $47.2M | 6 |
| SPORTS LEADERSHIP AND MANAGEMENT INC | Miami | B200 Education | $47.1M | 5 |
| AMERICAN WELDING SOCIETY INC | Miami | S410 Community Improvement & Capacity Building | $46.8M | 10 |
| PALM BEACH COUNTY FIRE FIGHTERS | West Palm Beach | — | $46.7M | 8 |
| GENESIS HEALTH DEVELOPMENT INC | Jacksonville | — | $46.6M | 9 |
| ESCAMBIA COMMUNITY CLINICS INC | Pensacola | E32Z Health Care | $46.4M | 12 |
| HEALTHY START MOMCARE NETWORK INC | Tallahassee | P40 Human Services | $46.4M | 3 |
| STRATUM HEALTH SYSTEM INC | Lakewood Ranch | E19 Health Care | $46.3M | 12 |
| FLORIDA ASSOCIATION OF REALTORS | Orlando | S41 Community Improvement & Capacity Building | $46.2M | 9 |
| Emergency Assistance Foundation Inc | West Palm Beach | P60 Human Services | $46.1M | 1 |
| Early Learning Coalition of Pasco and Hernando Counties Inc | Hudson | B12 Education | $46.1M | 4 |
| HEART OF FLORIDA HEALTH CENTER INC | Ocala | E32Z Health Care | $45.8M | 14 |
| GOODWILL INDUSTRIES OF NORTH FLORIDAINC | Jacksonville | — | $45.7M | 11 |
| UNITED CEREBRAL PALSY OF CENTRAL | Orlando | G23Z Diseases, Disorders & Medical Disciplines | $45.7M | 3 |
| THE RONALD & JOYCE WANEK FOUNDATION LTD | Tampa | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $45.6M | 0 |
| COMMUNITY BASED CARE OF BREVARD INC | Rockledge | E60 Health Care | $45.6M | 5 |
| FLORIDA PROTON THERAPY INSTITUTE INC | Jacksonville | E24 Health Care | $45.6M | 11 |
| APALACHEE CENTER INC | Tallahassee | F300 Mental Health & Crisis Intervention | $45.4M | 20 |
| Charles & Margery Barancik Foundation Inc | Sarasota | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $45.4M | 3 |
| THE YOUNG MEN'S CHRISTIAN ASSOCIATION OF | Jacksonville | — | $45.3M | 7 |
| The Jim Moran Foundation Inc | Deerfield Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $45.2M | 2 |
| THE GLENRIDGE ON PALMER RANCH INC | Sarasota | — | $45.1M | 5 |
| Metropolitan Charities Inc | St Petersburg | P800 Human Services | $45M | 9 |
| FLORIDA INTERNATIONAL UNIVERSITY | Miami | B110 Education | $44.9M | 13 |
| Morton Plant Mease Health Care Inc | Clearwater | E210 Health Care | $44.9M | 10 |
| EMPATH HEALTH INC | Lakewood Ranch | P117 Human Services | $44.8M | 14 |
| LOVE A CHILD INC | Fort Myers | Q330 International, Foreign Affairs & National Security | $44.5M | 5 |
| STATE CHARTERED CREDIT UNIONS IN FLORIDA | Pembroke Pines | — | $44.2M | 16 |
| Habitat for Humanity of Collier | Naples | L200 Housing & Shelter | $44M | 4 |
| Cox Science Center and Aquarium Inc | West Palm Beach | A570 Arts, Culture & Humanities | $43.9M | 2 |
| SCHOOL BOARD OF LEON COUNTY FL-VEBA | Tallahassee | — | $43.9M | 10 |
| GMP-EMPLOYERS RETIREE TRUST | Fort Myers | — | $43.8M | 1 |
| H LEE MOFFITT CANCER CENTER & RESEARCH | Tampa | G110 Diseases, Disorders & Medical Disciplines | $43.8M | 11 |
| AMATEUR ATHLETIC UNION OF THE US INC | Lake Buena Vista | — | $43.8M | 14 |
| BANYAN COMMUNITY HEALTH CENTER INC | Doral | E32 Health Care | $43.7M | 12 |
| THE AFFORDABLE HOUSING PRESERVATION | Jacksonville | L21 Housing & Shelter | $43.6M | 1 |
