| BLUELIGHT HEALS CORPORATION | Cheyenne | E70 Health Care | $4.6M | 7 |
| SHERIDAN COUNTY MEMORIAL HOSPITAL FOUNDATION | Sheridan | E112 Health Care | $4.6M | 3 |
| One22 Inc | Jackson | P20 Human Services | $4.4M | 1 |
| Wyoming Credit Union Ins TR | Casper | Y43 Mutual & Membership Benefit | $4.4M | 0 |
| HABITAT FOR HUMANITY OF THE GREATER TETON AREA | Jackson | — | $4.3M | 1 |
| Evanston Child Development Center | Evanston | — | $4.3M | 1 |
| Cloud Peak Counseling Center DBA OXBOW CENTER | Cody | F32Z Mental Health & Crisis Intervention | $4.3M | 1 |
| COUNCIL OF COMMUNITY SERVICES | Gillette | S200 Community Improvement & Capacity Building | $4.1M | 0 |
| GOODWILL INDUSTRIES OF WY INC | Cheyenne | J32Z Employment | $4M | 1 |
| CHEYENNE COUNTRY CLUB | Cheyenne | — | $4M | 0 |
| SENIOR CENTER OF JACKSON HOLE INC | Jackson | — | $4M | 2 |
| THE MARTIN FAMILY FOUNDATION C/O LARRY BEAN | Casper | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $3.8M | 0 |
| THE BUTTONWOOD FAMILY FOUNDATION | Wilson | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $3.7M | 0 |
| HABITAT FOR HUMANITY OF THE EASTERN BIGHORNS INC | Sheridan | — | $3.7M | 0 |
| Jackson Hole Community School | Jackson | B25 Education | $3.7M | 2 |
| WESTERN RESEARCH INSTITUTE | Laramie | B43 Education | $3.6M | 4 |
| Sweetwater County Child Developmental Center Inc | Green River | — | $3.6M | 1 |
| GRAND TETON ASSOCIATION | Moose | A56Z Arts, Culture & Humanities | $3.6M | 2 |
| SHERIDAN COMMUNITY LAND TRUST | Sheridan | C34 Environment | $3.6M | 1 |
| LUTHER CLASSICAL COLLEGE | Casper | B42 Education | $3.6M | 4 |
| THE WYLDLIFE FUND | Buffalo | D30 Animal-Related | $3.5M | 1 |
| LARAMIE COUNTY COMMUNITY COLLEGE FOUNDATION | Cheyenne | — | $3.5M | 4 |
| WYOMING EDUCATION ASSOCIATION | Cheyenne | — | $3.4M | 2 |
| CASPER COUNTRY CLUB | Casper | — | $3.4M | 1 |
| PIKE & SUSAN SULLIVAN CHARITABLE TRUST UAD 08/26/16 | Cheyenne | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $3.4M | 0 |
| LODGE NO 390 LARAMIE LOYAL ORDER OF MOOSE | Laramie | — | $3.4M | 0 |
| LYNN & FOSTER FRIESS FAMILY FOUNDATION | Jackson | X20Z Religion-Related | $3.4M | 1 |
| CHILD DEVELOPMENT CENTER - REGION II INC | Sheridan | E20Z Health Care | $3.3M | 0 |
| SOJOURNER TRUST | Jackson | — | $3.2M | 1 |
| Snowy Range Academy | Laramie | B29 Education | $3.2M | 2 |
| PODER ACADEMY | Cheyenne | B29 Education | $3.2M | 1 |
| ALLEN WYOMING FOUNDATION INC | Cody | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $3.2M | 1 |
| LARRY AND MARTHA DAY CHARITABLE TRU | DuBois | W12 Public & Societal Benefit | $3.2M | 0 |
| CODY STAMPEDE INC | Cody | — | $3.1M | 1 |
| BOYS AND GIRLS CLUB OF CHEYENNE WYOMING INC | Cheyenne | P20 Human Services | $3.1M | 3 |
| Senate Opportunity Fund | Cheyenne | — | $3.1M | 0 |
| JACKSON HOLE SKI & SNOWBOARD CLUB | Jackson | N60Z Recreation & Sports | $3.1M | 1 |
| THE WILDER TRUST | Jackson | C01 Environment | $3M | 1 |
| THE INSTITUTE FOR ETHNOMEDICINE INC DBA BRAIN CHEMISTRY LABS | Jackson | H99 Medical Research | $3M | 5 |
| TAIL WIND TRUST | Jackson | C01 Environment | $2.9M | 1 |
| GOLDEN HEART FOUNDATION | Jackson | T23 Philanthropy, Voluntarism & Grantmaking Foundations | $2.9M | 0 |
| CHILD DEVELOPMENT SERVICES OF FREMONT COUNTY | Lander | B22Z Education | $2.9M | 2 |
| Casper Family YMCA INC | Casper | — | $2.9M | 1 |
| The Doug Coombs Foundation | Jackson | O50 Youth Development | $2.9M | 1 |
| Astoria Park Conservancy | Jackson | C34 Environment | $2.8M | 3 |
| CHEYENNE ANIMAL SHELTER | Cheyenne | D200 Animal-Related | $2.8M | 1 |
| Homer A Scott & Mildred S Scott Foundation | Sheridan | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $2.8M | 2 |
| Thurston Family Foundation | Jackson | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $2.8M | 0 |
| Wyoming Classical Academy Inc | Mills | B29 Education | $2.8M | 0 |
| THE TVRC EDUCATION FOUNDATION INC | Jackson | O43 Youth Development | $2.8M | 1 |
