| NATIONAL FISH AND WILDLIFE FOUNDATION | Washington | DC | $513.2M | 11 |
| ZOOLOGICAL SOCIETY OF SAN DIEGO | San Diego | CA | $411M | 12 |
| THE AMERICAN SOCIETY FOR THE PREVENTION | New York | NY | $379.3M | 14 |
| Wildlife Conservation Society | Bronx | NY | $345.5M | 15 |
| Humane World for Animals Inc | Washington | DC | $207.9M | 18 |
| NATIONAL WILDLIFE FEDERATION | Reston | VA | $156.4M | 18 |
| Monterey Bay Aquarium Foundation | Monterey | CA | $155.9M | 18 |
| GEORGIA AQUARIUM INC & SUBSIDIARY | Atlanta | GA | $154M | 24 |
| BEST FRIENDS ANIMAL SOCIETY | Kanab | UT | $148.3M | 20 |
| MASSACHUSETTS SOCIETY FOR THE PREVENTION | Boston | MA | $144.8M | 12 |
| Chicago Zoological Society | Brookfield | IL | $108M | 12 |
| ROCKY MOUNTAIN ELK FOUNDATION INC | Missoula | MT | $100.1M | 10 |
| AMERICAN KENNEL CLUB INC | New York | NY | $99.6M | 23 |
| SHEDD AQUARIUM SOCIETY | Chicago | IL | $93M | 13 |
| THE ANIMAL MEDICAL CENTER | New York | NY | $91.9M | 10 |
| COLUMBUS ZOOLOGICAL PARK ASSOCIATION | Powell | OH | $88.8M | 10 |
| TROUT UNLIMITED INC | Arlington | VA | $83.5M | 9 |
| Houston Zoo Inc | Houston | TX | $82.5M | 9 |
| OMAHA ZOOLOGICAL SOCIETY | Omaha | NE | $82M | 10 |
| PEOPLE FOR THE ETHICAL TREATMENT | Norfolk | VA | $77.2M | 8 |
| GREAT LAKES FISHERY COMMISSION | Ann Arbor | MI | $75.6M | 6 |
| SAN DIEGO HUMANE SOCIETY & SPCA | San Diego | CA | $72.9M | 8 |
| ZOOLOGICAL SOCIETY OF CINCINNATI | Cincinnati | OH | $69.4M | 11 |
| PETSMART CHARITIES INC | Phoenix | AZ | $68.7M | 0 |
| GOVERNORS ISLAND CORPORATION | New York | NY | $66.1M | 9 |
| NEW ENGLAND AQUARIUM CORPORATION | Boston | MA | $65.4M | 15 |
| WOODLAND PARK ZOOLOGICAL SOCIETY | Seattle | WA | $64.7M | 15 |
| SAN ANTONIO ZOOLOGICAL SOCIETY | San Antonio | TX | $63.8M | 10 |
| THE SAINT LOUIS ZOO ASSOCIATION | Saint Louis | MO | $63.3M | 10 |
| DENVER ZOOLOGICAL FOUNDATION | Denver | CO | $60.1M | 15 |
| AQUARIUM OF THE PACIFIC CORPORATION | Long Beach | CA | $57M | 12 |
| LINCOLN PARK ZOOLOGICAL SOCIETY | Chicago | IL | $56.4M | 8 |
| NATIONAL AQUARIUM INC | Baltimore | MD | $55.6M | 11 |
| Ahimsa Foundation | Niwot | CO | $55.1M | 0 |
| DETROIT ZOOLOGICAL SOCIETY | Royal Oak | MI | $54.3M | 12 |
| FRIENDS OF THE ZOO INC OF KANSAS CITY | Kansas City | MO | $53.6M | 9 |
| THE SEATTLE AQUARIUM SOCIETY | Seattle | WA | $53.3M | 13 |
| REWILD | Austin | TX | $53.3M | 8 |
| THE FORT WORTH ZOOLOGICAL ASSOCIATION | Fort Worth | TX | $52.8M | 8 |
| WILDLIFE CONSERVATION NETWORK INC | San Francisco | CA | $52.5M | 7 |
| Arizona Humane Society | Phoenix | AZ | $52.2M | 11 |
| INDIANAPOLIS ZOOLOGICAL SOCIETY INC | Indianapolis | IN | $50.1M | 9 |
| THE TOLEDO ZOOLOGICAL SOCIETY | Toledo | OH | $50M | 9 |
| Lowry Park Zoological Society of Tampa | Tampa | FL | $48.6M | 10 |
| ARIZONA CENTER FOR NATURE CONSERVATION | Phoenix | AZ | $46.7M | 8 |
| THE SAN FRANCISCO SOCIETY FOR THE | San Francisco | CA | $46M | 17 |
| CENTER FOR BIOLOGICAL DIVERSITY INC | Tucson | AZ | $45.7M | 11 |
| NORTH SHORE ANIMAL LEAGUE AMERICA INC | Port Washington | NY | $45.1M | 11 |
| Audubon Nature Institute Inc | New Orleans | LA | $44.9M | 9 |
| ZOOLOGICAL SOCIETY OF PHILADELPHIA | Philadelphia | PA | $43.4M | 14 |
