| COMMONSPIRIT HEALTH | Englewood | E210 Health Care | $3.4B | 44 |
| CATHOLIC HEALTH INITIATIVES COLORADO | Centennial | E220 Health Care | $3.3B | 30 |
| Children's Hospital Colorado | Aurora | E240 Health Care | $1.9B | 17 |
| Avenir Foundation Inc | Lakewood | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $1.2B | 0 |
| INTERMOUNTAIN FRONT RANGE INC | Broomfield | E220 Health Care | $1.2B | 30 |
| POUDRE VALLEY MEDICAL GROUP LLC | Fort Collins | E32 Health Care | $1B | 15 |
| ALLIANCE FOR SUSTAINABLE ENERGY LLC | Golden | U05 Science & Technology | $1B | 10 |
| SISTERS OF CHARITY OF LEAVENWORTH HEALTH SYSTEM INC | Broomfield | E12 Health Care | $990.7M | 44 |
| POUDRE VALLEY HEALTH CARE INC | Fort Collins | E220 Health Care | $970.2M | 18 |
| UNIVERSITY OF DENVER | Denver | B430 Education | $834.9M | 30 |
| Portercare Adventist Health System | Greenwood Village | E220 Health Care | $731.2M | 27 |
| DELTA DENTAL OF COLORADO | Centennial | E11 Health Care | $680.9M | 24 |
| COLORADO ACCESS | Aurora | — | $667.1M | 20 |
| SAINT JOSEPH HOSPITAL INC | Denver | E220 Health Care | $645.4M | 28 |
| MEDICAL CENTER OF THE ROCKIES | Fort Collins | E22 Health Care | $642M | 18 |
| ENT CREDIT UNION | Colorado Springs | — | $594.7M | 24 |
| DENVER HEALTH MEDICAL PLAN INC | Denver | — | $536M | 15 |
| PARKVIEW MEDICAL CENTER | Pueblo | E220 Health Care | $518.8M | 8 |
| ST MARY'S HOSPITAL & MEDICAL CENTER INC | Grand Junction | E220 Health Care | $515.2M | 17 |
| HEXAGON FOUNDATION | Colorado Springs | X20 Religion-Related | $506M | 3 |
| Young Life | Colorado Springs | O550 Youth Development | $505M | 14 |
| BELLCO CREDIT UNION | Greenwood Village | — | $496.7M | 25 |
| UNITED STATES OLYMPIC AND PARALYMPIC COMMITTEE | Colorado Springs | N710 Recreation & Sports | $496.3M | 27 |
| BOULDER COMMUNITY HEALTH | Boulder | E220 Health Care | $488.7M | 15 |
| UNIVERSITY OF COLORADO FOUNDATION | Denver | B110 Education | $456.1M | 7 |
| COMMONSPIRIT MOUNTAIN REGION FKA CENTURA HEALTH CORPORATION | Centennial | E210 Health Care | $418.4M | 24 |
| United Power Inc | Brighton | — | $416.1M | 25 |
| CORE ELECTRIC COOPERATIVE | Sedalia | — | $413.2M | 26 |
| NATIONAL JEWISH HEALTH | Denver | E240 Health Care | $401.1M | 18 |
| VAIL CLINIC INC % VHH ACCOUNTING DEPARTMENT | Vail | E220 Health Care | $389.3M | 14 |
| VALLEY VIEW HOSPITAL ASSOCIATION | Glenwood Springs | E220 Health Care | $368.5M | 10 |
| CANVAS CREDIT UNION | Lone Tree | — | $360.6M | 23 |
| Colorado West Healthcare System | Grand Junction | E220 Health Care | $312.5M | 15 |
| UNIVERSITY CORPORATION FOR ATMOSPHERIC RESEARCH | Boulder | U300 Science & Technology | $311M | 21 |
| THE COLORADO COLLEGE | Colorado Springs | B430 Education | $294.1M | 21 |
| CHRISTIAN COMMUNITY FOUNDATION INC | Colorado Springs | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $263.2M | 6 |
| UCHEALTH GREELEY HOSPITAL | Fort Collins | E20 Health Care | $238.3M | 10 |
| CHARTER FUND INC D/B/A CHARTER SCHOOL GROWTH FUND | Denver | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $232.4M | 11 |
| ELEVATIONS CREDIT UNION | Boulder | — | $216.4M | 22 |
| BRIGHTON COMMUNITY HOSPITAL ASSOCIATION | Brighton | E220 Health Care | $211.6M | 14 |
| THE DENVER FOUNDATION | Denver | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $208.3M | 10 |
| PAHS Service Corporation | Greenwood Village | — | $199.7M | 10 |
| MONTROSE MEMORIAL HOSPITAL INC | Montrose | E20 Health Care | $197.4M | 7 |
| DANIELS FUND | Denver | T99 Philanthropy, Voluntarism & Grantmaking Foundations | $184.5M | 18 |
| FOOD BANK OF THE ROCKIES | Denver | K31Z Food, Agriculture & Nutrition | $174M | 12 |
| Goodwill of Colorado | Colorado Springs | J32Z Employment | $173.5M | 10 |
| Allosource | Centennial | E650 Health Care | $172.7M | 13 |
| REGIS UNIVERSITY | Denver | B20 Education | $165.6M | 11 |
| Craig Hospital | Englewood | E220 Health Care | $164.8M | 11 |
| Rocky Mountain Institute | Boulder | — | $164.7M | 13 |
