| COMMONSPIRIT HEALTH | Englewood | E210 Health Care | $3.9B | 47 |
| Catholic Health Initiatives Colorado | Centennial | E220 Health Care | $2.6B | 23 |
| Children's Hospital Colorado | Aurora | E240 Health Care | $1.9B | 17 |
| INTERMOUNTAIN FRONT RANGE INC | Broomfield | E220 Health Care | $1.1B | 30 |
| POUDRE VALLEY MEDICAL GROUP LLC | Fort Collins | E32 Health Care | $987.6M | 14 |
| SISTERS OF CHARITY OF LEAVENWORTH HEALTH | Broomfield | E12 Health Care | $898.1M | 46 |
| POUDRE VALLEY HEALTH CARE INC | Fort Collins | E220 Health Care | $842.3M | 26 |
| UNIVERSITY OF DENVER | Denver | B430 Education | $807.3M | 33 |
| ALLIANCE FOR SUSTAINABLE ENERGY LLC | Golden | U05 Science & Technology | $786.3M | 10 |
| Portercare Adventist Health System | Greenwood Village | E220 Health Care | $731.2M | 27 |
| DELTA DENTAL OF COLORADO | Centennial | E11 Health Care | $680.9M | 24 |
| COLORADO ACCESS | Aurora | — | $667.1M | 20 |
| SAINT JOSEPH HOSPITAL INC | Denver | E220 Health Care | $645.4M | 28 |
| MEDICAL CENTER OF THE ROCKIES | Fort Collins | E22 Health Care | $604.1M | 16 |
| ENT CREDIT UNION | Colorado Springs | — | $594.7M | 24 |
| THE RYAN FOUNDATION | Greenwood Village | — | $561.6M | 3 |
| DENVER HEALTH MEDICAL PLAN INC | Denver | — | $539.3M | 13 |
| PARKVIEW MEDICAL CENTER | Pueblo | E220 Health Care | $537.3M | 9 |
| CENTURA HEALTH CORPORATION | Centennial | E210 Health Care | $527.6M | 13 |
| ST MARY'S HOSPITAL & MEDICAL | Grand Junction | E220 Health Care | $515.2M | 17 |
| Young Life | Colorado Springs | O550 Youth Development | $505M | 14 |
| BELLCO CREDIT UNION | Greenwood Village | — | $496.7M | 25 |
| UNITED STATES OLYMPIC AND PARALYMPIC | Colorado Springs | N710 Recreation & Sports | $496.3M | 27 |
| Boulder Community Health | Boulder | E220 Health Care | $458M | 14 |
| UNIVERSITY OF COLORADO FOUNDATION | Denver | B110 Education | $456.1M | 7 |
| CORE ELECTRIC COOPERATIVE | Sedalia | — | $413.2M | 26 |
| VAIL CLINIC INC | Vail | E220 Health Care | $389.3M | 14 |
| NATIONAL JEWISH HEALTH | Denver | E240 Health Care | $348.5M | 17 |
| VALLEY VIEW HOSPITAL ASSOCIATION | Glenwood Springs | E220 Health Care | $345.5M | 10 |
| United Power Inc | Brighton | — | $342.5M | 22 |
| UNIVERSITY CORPORATION FOR ATMOSPHERIC | Boulder | U300 Science & Technology | $311M | 21 |
| CANVAS CREDIT UNION | Lone Tree | — | $308M | 21 |
| Colorado West Healthcare System | Grand Junction | E220 Health Care | $268.8M | 16 |
| THE COLORADO COLLEGE | Colorado Springs | B430 Education | $263.3M | 18 |
| CHRISTIAN COMMUNITY FOUNDATION INC | Colorado Springs | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $263.2M | 6 |
| BRIGHTON COMMUNITY HOSPITAL ASSOCIATION | Brighton | E220 Health Care | $211.6M | 14 |
| PAHS Service Corporation | Greenwood Village | — | $199.7M | 10 |
| UCHEALTH GREELEY HOSPITAL | Fort Collins | E20 Health Care | $194.3M | 10 |
| ELEVATIONS CREDIT UNION | Boulder | — | $190.5M | 21 |
| MONTROSE MEMORIAL HOSPITAL INC | Montrose | E20 Health Care | $174.8M | 7 |
| Goodwill of Colorado | Colorado Springs | J32Z Employment | $173.5M | 10 |
| Allosource | Centennial | E650 Health Care | $172.7M | 13 |
| REGIS UNIVERSITY | Denver | B20 Education | $165.6M | 11 |
| Craig Hospital | Englewood | E220 Health Care | $164.8M | 11 |
| Holy Cross Electric Association Inc | Glenwood Springs | — | $159.4M | 18 |
| Colorado Christian University | Lakewood | B430 Education | $156.6M | 9 |
| FOOD BANK OF THE ROCKIES | Denver | K31Z Food, Agriculture & Nutrition | $151.9M | 10 |
| MENTAL HEALTH CENTER OF DENVER | Denver | F30Z Mental Health & Crisis Intervention | $149M | 12 |
| POUDRE VALLEY REA INC | Fort Collins | — | $144.8M | 16 |
| CHARTER FUND INC | Broomfield | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $141.9M | 5 |
