| CENTRAL WYOMING COLLEGE FOUNDATION | Riverton | — | $9.2M | 2 |
| EMERALD GATE CHARITABLE TRUST | Jackson | T90 Philanthropy, Voluntarism & Grantmaking Foundations | $8.8M | 1 |
| WESTERN FUTURES FUND INC | Sheridan | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $8.7M | 0 |
| Cathedral Home for Children | Laramie | P700 Human Services | $8.6M | 3 |
| Central Wyoming Hospice & Transitions | Casper | — | $8.3M | 1 |
| LARAMIE COUNTY LIBRARY FOUNDATION | Cheyenne | B71I Education | $8.1M | 2 |
| CIOS | Wilson | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $8M | 3 |
| JACKSON HOLE LAND TRUST | Jackson | C340 Environment | $7.9M | 3 |
| YOUTH EMERGENCY SERVICES INC | Gillette | P73Z Human Services | $7.8M | 1 |
| CLIMB DBA CLIMB WYOMING | Cheyenne | P42 Human Services | $7.7M | 2 |
| WESTWARD HEIGHTS | Lander | E91Z Health Care | $7.6M | 1 |
| CHEYENNE HEALTH & WELLNESS CENTER | Cheyenne | E32 Health Care | $7.6M | 8 |
| NORTHWEST COMMUNITY ACTION PROGRAMS OF WYOMING | Worland | P20Z Human Services | $7.5M | 2 |
| BLUE GRIT | Cheyenne | B30 Education | $7.4M | 3 |
| Ivinson Memorial Hospital Foundation | Laramie | — | $7.4M | 0 |
| THE CHILDREN'S LEARNING CENTER INC | Jackson | — | $7.4M | 1 |
| Child Development Center of Natrona County | Casper | — | $7.3M | 1 |
| NATRONA COLLECTIVE HEALTH TRUST | Casper | E12 Health Care | $7.2M | 4 |
| CASPER COLLEGE FOUNDATION | Casper | B110 Education | $7.2M | 3 |
| WIND RIVER TRIBAL BUFFALO INITIATIVE | Kinnear | C60 Environment | $6.9M | 0 |
| Sheridan County Young Mens Christian Association | Sheridan | P27Z Human Services | $6.7M | 1 |
| NATIONAL MUSEUM OF WILDLIFE ART OF THE UNITED STATES | Jackson | A50 Arts, Culture & Humanities | $6.7M | 5 |
| Boys & Girls Club of Central Wyoming | Casper | O23Z Youth Development | $6.6M | 1 |
| STRIDE LEARNING CENTER | Cheyenne | B28Z Education | $6.3M | 2 |
| Wyoming Chambers Health Benefit Association | Gillette | S41 Community Improvement & Capacity Building | $6.1M | 0 |
| Pamela and Wayne Garrison Family Foundation | Big Horn | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $6M | 1 |
| SOBECKI FAMILY FOUNDATION | Jackson | I12 Crime & Legal-Related | $5.9M | 0 |
| ALTERNATIVE ELDER LIVING INC | Sheridan | — | $5.8M | 2 |
| SUBLETTE COUNTY HEALTH FOUNDATION | Pinedale | E12 Health Care | $5.7M | 0 |
| COWBOY JOE CLUB | Laramie | B43I Education | $5.5M | 0 |
| CHILDREN'S RESOURCE CENTER | Powell | B28Z Education | $5.5M | 0 |
| LINCOLN UINTA CHILD DEVELOPMENT ASSOCIATION | Mountain View | B22Z Education | $5.5M | 1 |
| WYOMING HEALTH FAIRS | Casper | — | $5.4M | 1 |
| TETON YOUTH AND FAMILY SERVICES | Jackson | F33Z Mental Health & Crisis Intervention | $5.4M | 4 |
| GARLAND LIGHT & POWER COMPANY | Powell | — | $5.3M | 10 |
| ABSAROKA HEAD START | Worland | — | $5.3M | 2 |
| SENIOR CITIZEN COUNCIL | Sheridan | P81Z Human Services | $5.3M | 1 |
| CHALTEN TRUST | Jackson | C01 Environment | $5.3M | 1 |
| MAGIC CITY ENTERPRISES INC | Cheyenne | J300 Employment | $5.2M | 1 |
| DEVELOPMENTAL PRESCHOOL AND DAY CARE | Laramie | P33 Human Services | $5.2M | 1 |
| WHITNEY BENEFITS INC AND SUBSIDIARY | Sheridan | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $5.2M | 1 |
| Wyoming Rescue Mission | Casper | L41Z Housing & Shelter | $5.1M | 1 |
| LINCOLN SELF RELIANCE INC | Afton | P82 Human Services | $5M | 1 |
| WYOMING WEATHERIZATION SERVICES | Worland | L81 Housing & Shelter | $4.9M | 2 |
| MENTAL HEALTH & RECOVERY SERVICES OF JACKSON HOLE | Jackson | — | $4.9M | 5 |
| SILVER DOLLAR FOUNDATION | Jackson | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $4.8M | 0 |
| CORBETT MEDICAL FOUNDATION | Saratoga | E80Z Health Care | $4.7M | 0 |
| WILLIAM H AND CARRIE GOTTSCHE FOUNDATION | Thermopolis | T90Z Philanthropy, Voluntarism & Grantmaking Foundations | $4.7M | 1 |
| BIG HORN ENTERPRISES INC | Thermopolis | P820 Human Services | $4.7M | 1 |
| IKIGAI FOUNDATION | Teton Village | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $4.6M | 0 |
