| SUTTER BAY MEDICAL FOUNDATION | Sacramento | CA | $3.5B | 23 |
| Dana-Farber Cancer Institute Inc | Boston | MA | $3.5B | 19 |
| Howard Hughes Medical Institute | Chevy Chase | MD | $1.8B | 33 |
| CHAN ZUCKERBERG BIOHUB INC | San Francisco | CA | $1.3B | 21 |
| HEALTH RESEARCH INC | Menands | NY | $1.1B | 9 |
| THE BROAD INSTITUTE INC | Cambridge | MA | $860.4M | 13 |
| THE SCRIPPS RESEARCH INSTITUTE | La Jolla | CA | $641.1M | 15 |
| HENRY M JACKSON FOUNDATION FOR THE ADVANCEMENT OF MILITARY MEDICINE | Bethesda | MD | $628.9M | 13 |
| The Jackson Laboratory | Bar Harbor | ME | $572.8M | 14 |
| THE MICHAEL J FOX FOUNDATION | New York | NY | $505.3M | 11 |
| BECKMAN RESEARCH INSTITUTE OF THE CITY OF HOPE | Duarte | CA | $285.3M | 21 |
| ALLEN INSTITUTE | Seattle | WA | $272.1M | 10 |
| PUBLIC HEALTH SOLUTIONS | New York | NY | $271.2M | 9 |
| CHDI Foundation Inc | Roseland | NJ | $206.6M | 1 |
| RIVERSIDE RESEARCH INSTITUTE | Fairfax | VA | $189.3M | 25 |
| THE FEINSTEIN INSTITUTES FOR | Westbury | NY | $163.3M | 10 |
| ELIZABETH GLASER PEDIATRIC AIDS FOUNDATION | Washington | DC | $162.3M | 19 |
| Dana-FarberMass General Brigham Cancer Care INC | Boston | MA | $137.6M | 12 |
| American Association for Cancer Research Inc | Philadelphia | PA | $137.4M | 9 |
| GREATER DELAWARE VALLEY SOCIETY OF | Philadelphia | PA | $134.5M | 9 |
| WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH | Cambridge | MA | $120.2M | 12 |
| THE J DAVID GLADSTONE INSTITUTES | San Francisco | CA | $119.2M | 17 |
| SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE | La Jolla | CA | $117.2M | 12 |
| Oklahoma Medical Research Foundation | Oklahoma City | OK | $114.3M | 18 |
| VAN ANDEL RESEARCH INSTITUTE | Grand Rapids | MI | $112.7M | 15 |
| LOVELACE BIOMEDICAL RESEARCH INSTITUTE | Albuquerque | NM | $112.1M | 9 |
| COMMONWEALTH FOUNDATION FOR CANCER | Richmond | VA | $102.3M | 2 |
| The Institute For Cancer Research | Philadelphia | PA | $98.8M | 14 |
| THE WISTAR INSTITUTE OF ANATOMY AND | Philadelphia | PA | $93.1M | 15 |
| LUNDQUIST INSTITUTE FOR BIOMEDICAL | Torrance | CA | $91.6M | 17 |
| CHILDREN'S RESEARCH INSTITUTE | Washington | DC | $89.7M | 10 |
| PARALYZED VETERANS OF AMERICA | Washington | DC | $86.4M | 22 |
| THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE | Phoenix | AZ | $85.9M | 17 |
| THE MORGRIDGE INSTITUTE FOUNDATION INC | Madison | WI | $84.9M | 3 |
| TEXAS BIOMEDICAL RESEARCH INSTITUTE | San Antonio | TX | $83.2M | 12 |
| BENAROYA RESEARCH INSTITUTE AT VIRGINIA | Seattle | WA | $82.5M | 9 |
| MEDICAL UNIVERSITY OF SOUTH CAROLINA | Charleston | SC | $80.6M | 3 |
| CARDIOVASCULAR RESEARCH FOUNDATION | New York | NY | $78.3M | 8 |
| Baylor Research Institute | Dallas | TX | $73.3M | 13 |
| THE GOG FOUNDATION INC | Edgewater | MD | $66.1M | 13 |
| Stanley Manne Childrens Research Institute | Chicago | IL | $63.1M | 5 |
| THE V FOUNDATION | Cary | NC | $62.4M | 9 |
| Foundation for the National Institutes of Health Inc | North Bethesda | MD | $61M | 13 |
| PHILADELPHIA FIGHT | Philadelphia | PA | $60.6M | 10 |
| GLOBAL ALLIANCE FOR TB DRUG DEVELOPMENT INC | New York | NY | $60.2M | 12 |
| DEAN MCGEE EYE INSTITUTE | Oklahoma City | OK | $57.4M | 9 |
| Parkinson's Foundation Inc | New York | NY | $56M | 14 |
| USONA INSTITUTE INC | Madison | WI | $54.9M | 0 |
| HENNEPIN HEALTHCARE RESEARCH INSTITUTE | Minneapolis | MN | $54.7M | 18 |
| ARC RESEARCH INSTITUTE | Palo Alto | CA | $53.6M | 16 |
