| Aleo Network Foundation | Cheyenne | — | $260.5M | 6 |
| POWDER RIVER ENERGY CORPORATION | Sundance | — | $176.2M | 21 |
| IVINSON MEMORIAL HOSPITAL | Laramie | E22 Health Care | $161.6M | 9 |
| WYOMING EDUCATORS' BENEFIT TRUST | Cheyenne | — | $139.2M | 1 |
| Cumming Foundation | Jackson | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $121.8M | 1 |
| UNIVERSITY OF WYOMING FOUNDATION | Laramie | B110 Education | $113.6M | 13 |
| HIGH PLAINS POWER INC | Riverton | — | $81M | 17 |
| POWELL VALLEY HEALTH CARE INC | Powell | E20 Health Care | $79.7M | 10 |
| Lower Valley Energy Inc | Afton | — | $77.9M | 16 |
| HIGH WEST ENERGY INC | Pine Bluffs | — | $73.9M | 14 |
| PEOPLE TO PEOPLE AID CORP | Cheyenne | E80 Health Care | $57.3M | 0 |
| VOLUNTEERS OF AMERICA NORTHERN ROCKIES | Sheridan | — | $56.7M | 11 |
| COMMUNITY FOUNDATION OF JACKSON HOLE | Jackson | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $53.1M | 7 |
| DELTA DENTAL PLAN OF WYOMING | Cheyenne | — | $46.7M | 15 |
| NATIONAL OUTDOOR LEADERSHIP SCHOOL | Lander | C600 Environment | $43M | 10 |
| HOLDFAST TRUST | Jackson | C01 Environment | $41.4M | 1 |
| Hot Springs County Hospital District | Thermopolis | E22 Health Care | $35.5M | 8 |
| GRAND TETON NATIONAL PARK FOUNDATION | Moose | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $34M | 6 |
| Buffalo Bill Memorial Association | Cody | A540 Arts, Culture & Humanities | $32.5M | 4 |
| WYRULEC COMPANY | Torrington | — | $26.2M | 11 |
| CHEYENNE FRONTIER DAYS INC | Cheyenne | — | $25.5M | 3 |
| THE ISSA FOUNDATION | Cheyenne | P20 Human Services | $24.9M | 0 |
| WYOMING COMMUNITY FOUNDATION | Laramie | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $23M | 3 |
| WYO COMPLEX | Casper | N30 Recreation & Sports | $22.5M | 1 |
| Wheatland Rural Electric Association Inc | Wheatland | — | $20.4M | 9 |
| COMMUNITY HEALTH CENTER OF CENTRAL | Casper | E320 Health Care | $20.2M | 8 |
| EMERALD GATE CHARITABLE TRUST | Jackson | T90 Philanthropy, Voluntarism & Grantmaking Foundations | $20M | 1 |
| CARBON POWER & LIGHT INC | Saratoga | — | $17.6M | 16 |
| Big Horn Rural Electric Company | Basin | — | $17.4M | 12 |
| WALKING SOFTER | Jackson | C99 Environment | $17.2M | 3 |
| Niobrara Electric Association Inc | Lusk | W80 Public & Societal Benefit | $17.1M | 15 |
| TETON SCIENCE SCHOOLS INC | Jackson | C60Z Environment | $16.2M | 6 |
| WYOMING MACHINERY COMPANY | Casper | — | $15.5M | 0 |
| GRAND TETON MUSIC FESTIVAL INC | Jackson | A680 Arts, Culture & Humanities | $15.4M | 5 |
| WYOMING CHILD & FAMILY DEVELOPMENT INC | Guernsey | B28Z Education | $14.8M | 0 |
| Bridger Valley Electric Association Inc | Mountain View | W80Z Public & Societal Benefit | $14.3M | 0 |
| CENTRAL WYOMING COUNSELING CENTER | Casper | F30Z Mental Health & Crisis Intervention | $13.9M | 6 |
| CHEYENNE HEALTH & WELLNESS CENTER | Cheyenne | E32 Health Care | $13.1M | 8 |
| WYOMING STOCK GROWERS LAND TRUST | Cheyenne | K250 Food, Agriculture & Nutrition | $12.9M | 1 |
| Fundamental Wellbeing Foundation | Jackson | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $12.8M | 1 |
| TETON YOUTH AND FAMILY SERVICES | Jackson | F33Z Mental Health & Crisis Intervention | $12.6M | 5 |
| SPINAL MUSCULAR ATROPHY FOUNDATION | Jackson | B99 Education | $12.5M | 0 |
| St John's Health Foundation | Jackson | E112 Health Care | $12.1M | 2 |
| High Country Behavioral Health | Afton | F32 Mental Health & Crisis Intervention | $11.3M | 11 |
| WESTERN STATES LEARNING CORPORATION | Cheyenne | B82 Education | $11.1M | 3 |
| ST JOSEPH'S CHILDREN'S HOME INC | Torrington | F330 Mental Health & Crisis Intervention | $10.8M | 2 |
| WHITNEY BENEFITS INC | Sheridan | — | $10.8M | 1 |
| Hughes Charitable Foundation | Jackson | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $10.2M | 1 |
| JACKSON HOLE COMMUNITY HOUSING TRUST | Jackson | — | $10.1M | 1 |
| JACKSON HOLE CLASSICAL ACADEMY INC | Jackson | B24 Education | $10M | 2 |
