| CALVIN COMMUNITY | Des Moines | — | $4.3M | 5 |
| Raam Raj Foundation | Fairfield | Q21 International, Foreign Affairs & National Security | $4.3M | 3 |
| MISSOURI RIVER HISTORICAL DEVELOPMENT | Sioux City | — | $4.3M | 7 |
| BOARD OF TRUSTEES -GRAND CHARITY FU | Cedar Rapids | — | $4.2M | 1 |
| CEDAR RAPIDS METRO | Cedar Rapids | — | $4.2M | 4 |
| IOWA CATTLEMENS ASSN - GROUP RETURN | Ames | — | $4.2M | 0 |
| THE FRIENDSHIP VILLAGE CHARITABLE | Waterloo | L22 Housing & Shelter | $4.2M | 2 |
| REGIONAL WATER | Avoca | — | $4.2M | 0 |
| CEDAR RAPIDS PUBLIC LIBRARY FOUNDATION | Cedar Rapids | B11 Education | $4.2M | 1 |
| BOYS AND GIRLS CLUBS OF STORY COUNTY INC | Ames | O23Z Youth Development | $4.2M | 1 |
| BOYS & GIRLS CLUBS OF CENTRAL IOWA | Des Moines | O21I Youth Development | $4.2M | 1 |
| HEARTLAND GOODWILL ENTERPRISES | Cedar Rapids | P45 Human Services | $4.1M | 2 |
| THE SWARM COLLECTIVE INC | North Liberty | T50 Philanthropy, Voluntarism & Grantmaking Foundations | $4.1M | 1 |
| ARENACO | Coralville | N30 Recreation & Sports | $4.1M | 0 |
| SCENIC ACRES | St Olaf | P70Z Human Services | $4.1M | 1 |
| IOWA PIPE TRADES & HVAC TRAINING | Des Moines | — | $4.1M | 6 |
| GREATER ALTOONA COMM SERVICE CAMPUS | Altoona | N310 Recreation & Sports | $4.1M | 1 |
| Bergman Academy | Des Moines | — | $4.1M | 2 |
| INTERNATIONAL ASSOCIATION FOR FOOD | Des Moines | — | $4.1M | 2 |
| WEST CENTRAL IOWA RURAL WATER | Manning | — | $4.1M | 12 |
| OUTREACH INC | Union | Q30 International, Foreign Affairs & National Security | $4.1M | 5 |
| Athene Charitable Foundation | West Des Moines | T99 Philanthropy, Voluntarism & Grantmaking Foundations | $4.1M | 0 |
| MARSHALLTOWN YOUNG MEN'S CHRISTIAN | Marshalltown | — | $4.1M | 2 |
| HARRY VERMEER FAMILY FOUNDATION | Pella | — | $4.1M | 0 |
| EMCC Veba-Retiree Medical Benefits | Des Moines | Y43 Mutual & Membership Benefit | $4.1M | 1 |
| LIVING HISTORY FARMS FOUNDATION | Des Moines | A540 Arts, Culture & Humanities | $4.1M | 1 |
| INTERNATIONAL BROTHERHOOD OF TEAMSTERS | Cedar Rapids | — | $4.1M | 8 |
| DES MOINES PARKS & RECREATION FOUNDATION | Des Moines | N30Z Recreation & Sports | $4.1M | 0 |
| RIVER VALLEY CREDIT UNION | Ames | — | $4.1M | 3 |
| CHRISTIAN COMMUNITY DEVELOPMENT | Waterloo | Z99Z Unknown | $4.1M | 1 |
| GREATER DES MOINES PUBLIC ART FOUNDATION | Des Moines | A20 Arts, Culture & Humanities | $4M | 1 |
| FAMILY CRISIS CENTERS INC | Sioux Center | P43Z Human Services | $4M | 2 |
| ST LUKE'S HEALTH FOUNDATION OF | Sioux City | — | $4M | 5 |
| SUNFLOWER CHILDCARE CENTER INC | Decorah | P33Z Human Services | $4M | 2 |
| CHARLES AND HELEN YUNDT FOUNDATION | Vinton | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $3.9M | 0 |
| SAN DIEGO COUNTY PUBLIC SAFETY RETIREE | Clinton | Y43 Mutual & Membership Benefit | $3.9M | 0 |
| ANNA K MEREDITH ENDOWMENT TRUST | Des Moines | A110 Arts, Culture & Humanities | $3.9M | 1 |
| OPENING DOORS | Dubuque | — | $3.9M | 1 |
| RISE LTD | Elkader | J33Z Employment | $3.9M | 1 |
| MIND AND SPIRIT COUNSELING CENTER | Urbandale | — | $3.9M | 5 |
| SHEFFIELD CARE CENTER INC | Sheffield | P74Z Human Services | $3.9M | 1 |
| CHARLIE BROWN COMMUNITY DAY CARE CENTER INC | Mason City | — | $3.9M | 0 |
| TEEN CHALLENGE OF THE MIDLANDS INC | Colfax | F22Z Mental Health & Crisis Intervention | $3.9M | 1 |
| DOMESTIC VIOLENCE INTERVENTION PROGRAM | Iowa City | — | $3.8M | 2 |
| THE PRESBYTERIAN VILLAGE | Ackley | E910 Health Care | $3.8M | 1 |
| HOMES OF OAKRIDGE HUMAN SERVICES | Des Moines | P200 Human Services | $3.8M | 4 |
| BRIDGEVIEW COMMUNITY MENTAL HEALTH | Clinton | F32 Mental Health & Crisis Intervention | $3.8M | 3 |
| ALCOHOL & DRUG DEPENDENCY SERVICES | Burlington | F22Z Mental Health & Crisis Intervention | $3.8M | 1 |
| IOWA UNITED BASKETBALL | Des Moines | — | $3.8M | 1 |
| IOWA STATE UNIVERSITY ALUMNI ASSOCIATION | Ames | B84Z Education | $3.8M | 3 |
