| IOWA GIRLS HIGH SCHOOL ATHLETIC UNION | West Des Moines | N70Z Recreation & Sports | $4.6M | 4 |
| HERITAGE AREA AGENCY ON AGING | Cedar Rapids | P20 Human Services | $4.6M | 4 |
| New Visions Homeless Services | Council Bluffs | P20 Human Services | $4.5M | 3 |
| HORIZONS A FAMILY SERVICE ALLIANCE | Cedar Rapids | P73 Human Services | $4.5M | 2 |
| ORANGE CITY CHRISTIAN SCHOOL | Orange City | — | $4.5M | 0 |
| DES MOINES COUNCIL OF ST VINCENT DE PAUL SOCIETY | Windsor Heights | P20 Human Services | $4.5M | 1 |
| MARSHALLTOWN YOUNG MEN'S CHRISTIAN ASSOCIATION-YWCA | Marshalltown | — | $4.5M | 2 |
| JULIE GREEN MINISTRIES | Davenport | X80 Religion-Related | $4.5M | 4 |
| IOWA YOUTH ATHLETIC FOUNDATION | Ames | N620 Recreation & Sports | $4.5M | 3 |
| IN CONCERTCARE INC | Des Moines | E82B Health Care | $4.5M | 8 |
| International Union UAW Local 838 | Waterloo | — | $4.5M | 19 |
| BOYS AND GIRLS CLUBS OF THE CEDAR VALLEY | Waterloo | O21Z Youth Development | $4.4M | 1 |
| FELLOWSHIP VILLAGE | Inwood | P750 Human Services | $4.4M | 0 |
| IOWA HEARTLAND HABITAT FOR HUMANITY | Waterloo | L20Z Housing & Shelter | $4.4M | 1 |
| DES MOINES IRON WORKERS WELFARE FUND | Des Moines | — | $4.4M | 2 |
| GRAND HAVEN HOMES INC | Marion | — | $4.4M | 1 |
| PEO Program for Continuing Education | Des Moines | — | $4.3M | 0 |
| INTERNATIONAL BROTHERHOOD OF TEAMSTERS LOCAL UNION NO 238 | Cedar Rapids | — | $4.3M | 9 |
| HOSPICE OF NORTH IOWA | Mason City | P74Z Human Services | $4.3M | 9 |
| CALVIN COMMUNITY | Des Moines | — | $4.3M | 5 |
| MEMORIAL FOUNDATION OF ALLEN HOSPITAL | Waterloo | E112 Health Care | $4.2M | 7 |
| BOARD OF TRUSTEES -GRAND CHARITY FU GRAND LODGE OF IOWA AF & AM | Cedar Rapids | — | $4.2M | 1 |
| CEDAR RAPIDS METRO ECONOMIC ALLIANCE | Cedar Rapids | — | $4.2M | 4 |
| MISSOURI RIVER HISTORICAL DEVELOPMENT INC | Sioux City | — | $4.2M | 7 |
| IOWA CATTLEMENS ASSN - GROUP RETURN | Ames | — | $4.2M | 0 |
| OSSIAN SENIOR HOSPICE | Ossian | E91Z Health Care | $4.2M | 2 |
| REGIONAL WATER | Avoca | — | $4.2M | 0 |
| CEDAR VALLEY FRIENDS OF THE FAMILY INC | Waterloo | — | $4.2M | 2 |
| CHARLIE BROWN COMMUNITY DAYCARE CENTER INC | Mason City | — | $4.2M | 0 |
| BOYS & GIRLS CLUBS OF CENTRAL IOWA | Des Moines | O21I Youth Development | $4.2M | 1 |
| DOMESTIC VIOLENCE INTERVENTION PROGRAM | Iowa City | — | $4.1M | 2 |
| HEARTLAND GOODWILL ENTERPRISES | Cedar Rapids | P45 Human Services | $4.1M | 2 |
| PAINTERS & ALLIED TRADES DISTRICT | Cedar Rapids | Y43 Mutual & Membership Benefit | $4.1M | 0 |
| THE SWARM COLLECTIVE INC | North Liberty | T50 Philanthropy, Voluntarism & Grantmaking Foundations | $4.1M | 1 |
| NORTH STAR COMMUNITY SERVICES INC | Waterloo | J30Z Employment | $4.1M | 1 |
| SCENIC ACRES | St Olaf | P70Z Human Services | $4.1M | 1 |
| GREATER ALTOONA COMM SERVICE CAMPUS | Altoona | N310 Recreation & Sports | $4.1M | 1 |
| INTERNATIONAL ASSOCIATION FOR FOOD PROTECTION | Des Moines | — | $4.1M | 2 |
| SHEFFIELD CARE CENTER INC | Sheffield | P74Z Human Services | $4.1M | 1 |
| WEST CENTRAL IOWA RURAL WATER ASSOCIATION | Manning | — | $4.1M | 12 |
| OUTREACH INC | Union | Q30 International, Foreign Affairs & National Security | $4.1M | 5 |
| IOWA ASSOCIATION OF REALTORS | West Des Moines | — | $4.1M | 2 |
| THE FRIENDSHIP VILLAGE CHARITABLE TRUST | Waterloo | L22 Housing & Shelter | $4.1M | 2 |
| HARRY VERMEER FAMILY FOUNDATION | Pella | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $4.1M | 0 |
| EMCC Veba-Retiree Medical Benefits Master Trust | Des Moines | Y43 Mutual & Membership Benefit | $4.1M | 1 |
| LIVING HISTORY FARMS FOUNDATION | Des Moines | A540 Arts, Culture & Humanities | $4.1M | 1 |
| CHRISTIAN COMMUNITY DEVELOPMENT | Waterloo | Z99Z Unknown | $4.1M | 1 |
| GREATER DES MOINES PUBLIC ART FOUNDATION | Des Moines | A20 Arts, Culture & Humanities | $4M | 1 |
| FAMILY CRISIS CENTERS INC | Sioux Center | P43Z Human Services | $4M | 2 |
| THE BOB AND LIZ PETERSEN FAMILY FDN | Waterloo | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $4M | 0 |
