| CHRISTIAN LEAGUE FOR THE HANDICAPPED | Walworth | — | $5.9M | 1 |
| PAINTERS & ALLIED TRADES DISTRICT | Big Bend | — | $5.9M | 16 |
| BROADSCOPE DISABILITY SERVICES INC | Greenfield | G50Z Diseases, Disorders & Medical Disciplines | $5.9M | 1 |
| CONFLUENCE COUNCIL INC | Eau Claire | A61 Arts, Culture & Humanities | $5.9M | 1 |
| FIREFIGHTERS CREDIT UNION | Onalaska | — | $5.9M | 10 |
| RCS EMPOWERS INC | Sheboygan | E50Z Health Care | $5.8M | 3 |
| ISABEL BADER 2021 CHARITABLE TRUST | Milwaukee | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $5.8M | 1 |
| FEDERAL DEFENDER SERVICES OF WISCONSIN | Milwaukee | I80 Crime & Legal-Related | $5.8M | 6 |
| Green County Family YMCA Inc | Monroe | P27Z Human Services | $5.8M | 1 |
| ONE CITY SCHOOLS FOUNDATION INC | Madison | B12 Education | $5.8M | 1 |
| FROEDTERT MEMORIAL LUTHERAN HOSPITAL TRUST | Milwaukee | E110 Health Care | $5.8M | 3 |
| LEWIS W HYER TR | Milwaukee | — | $5.8M | 1 |
| MILWAUKEE SYMPHONY ORCHESTRA ENDOWMENT | Milwaukee | A110 Arts, Culture & Humanities | $5.8M | 0 |
| HUMANE ANIMAL WELFARE SOCIETY OF | Waukesha | D200 Animal-Related | $5.8M | 2 |
| NORTHWEST SIDE COMMUNITY DEVELOPMENT | Milwaukee | S310 Community Improvement & Capacity Building | $5.8M | 2 |
| BEYOND BLESSED PANTRY | Wisconsin Dells | K31 Food, Agriculture & Nutrition | $5.8M | 1 |
| International Chapter - | Hartland | — | $5.7M | 0 |
| DOOR COUNTY LAND TRUST INC | Sturgeon Bay | C340 Environment | $5.7M | 1 |
| NATIONAL CREDIT UNION FOUNDATION | Madison | — | $5.7M | 7 |
| RUNNING REBELS COMMUNITY | Milwaukee | O99Z Youth Development | $5.7M | 3 |
| WISCONSIN COMMUNITY ACTION PROGRAM | Madison | — | $5.7M | 1 |
| Young Mens Christian Association of the Northwoods Inc | Rhinelander | P27 Human Services | $5.7M | 1 |
| WISCONSIN JUDICARE INC | Wausau | I80Z Crime & Legal-Related | $5.7M | 3 |
| Easter Seals Southeast Wisconsin Inc | West Allis | — | $5.7M | 2 |
| International Union INTERNAL BROHD ELECT WKRS LOCAL 2150 | Menomonee Falls | — | $5.7M | 21 |
| LEGAL AID SOCIETY OF MILWAUKEE INC | Milwaukee | I83Z Crime & Legal-Related | $5.7M | 3 |
| BREWERY CREDIT UNION | Milwaukee | — | $5.7M | 1 |
| ORANGE CROSS AMBULANCE INC | Sheboygan | E62 Health Care | $5.7M | 1 |
| BADGER PRAIRIE NEEDS NETWORK INC | Verona | K31 Food, Agriculture & Nutrition | $5.7M | 0 |
| THE OSCAR RENNEBOHM FOUNDATION INC | Madison | — | $5.7M | 8 |
| IMPACT ALCOHOL AND OTHER DRUG ABUSE | Milwaukee | F20Z Mental Health & Crisis Intervention | $5.7M | 5 |
| SPECIAL OLYMPICS WISCONSIN INC | Madison | N72Z Recreation & Sports | $5.6M | 3 |
| UW Health ACO Inc | Middleton | E31 Health Care | $5.6M | 10 |
| BRAND FAMILY FOUNDATION | Madison | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $5.6M | 0 |
| SIEBERT LUTHERAN FOUNDATION INC | Milwaukee | — | $5.6M | 15 |
| WISCONSIN CENTER FOR MANUFACTURING | Madison | B99Z Education | $5.6M | 1 |
| THE ASSOCIATION OF STATE FLOODPLAIN | Madison | T99Z Philanthropy, Voluntarism & Grantmaking Foundations | $5.6M | 6 |
| TIME OF GRACE MINISTRY | Milwaukee | X99 Religion-Related | $5.5M | 5 |
| OZAUKEE COUNTRY CLUB | Mequon | N50 Recreation & Sports | $5.5M | 6 |
| OAKDALE CREDIT UNION | Oakdale | — | $5.5M | 8 |
| LIONS EYEBANK OF WISCONSIN INC | Madison | E65 Health Care | $5.5M | 4 |
| AMERICAN ACADEMY OF COSMETIC DENTISTRY | Monona | — | $5.5M | 4 |
| MADISON AREA TECHNICAL COLLEGE | Madison | B124 Education | $5.5M | 3 |
| ADVANCED EMPLOYMENT INC | Madison | J30Z Employment | $5.5M | 1 |
| ASSOCIATED BUILDERS & CONTRACTORS OF | Madison | — | $5.4M | 7 |
| FOND DU LAC AREA FOUNDATION | Fond Du Lac | — | $5.4M | 1 |
| Gundersen Lutheran Health System Inc | La Crosse | E21Z Health Care | $5.4M | 42 |
| WORLD DAIRY EXPO INC | Madison | B52Z Education | $5.4M | 2 |
| TOMORROWS CHILDREN INC | Waupaca | — | $5.4M | 2 |
| WISCONSIN YOUTH COMPANY INC | Madison | — | $5.4M | 1 |
