| NASSAU-PARADISE ISLAND PROMOTION BOARD | Plantation | S41 Community Improvement & Capacity Building | $37.2M | 5 |
| Premier Community HealthCare Group Inc | Dade City | E30Z Health Care | $37M | 12 |
| ARTIS-NAPLES INC | Naples | A610 Arts, Culture & Humanities | $36.9M | 11 |
| CENTRAL FLORIDA YOUNG MEN'S CHRISTIAN ASSOCIATION INC | Orlando | P27 Human Services | $36.9M | 12 |
| AREA AGENCY ON AGING OF PASCO-PINELLAS INC | St Petersburg | P810 Human Services | $36.9M | 5 |
| Florida Agricultural & Mechanical Univ Foundation Inc | Tallahassee | B110 Education | $36.8M | 4 |
| INSIGHT CREDIT UNION | Orlando | — | $36.8M | 7 |
| BLISS CARES INC | Orlando | H81 Medical Research | $36.6M | 7 |
| United Food and Commercial Workers Local 1000 and Kroger Dallas Health | Pembroke Pines | — | $36.5M | 0 |
| WESTMINSTER CHRISTIAN PRIVATE SCHOOL INC | Miami | B24Z Education | $36.4M | 6 |
| Orlando Health Foundation Inc | Orlando | E110 Health Care | $36.4M | 13 |
| AREA AGENCY OF AGING OF CENTRAL FL | Orlando | P81Z Human Services | $36.4M | 2 |
| EARLY LEARNING COALITION OF OSCEOLA COUNTY INC | Kissimmee | B99 Education | $36.3M | 4 |
| LIFE CONCEPTS INC D/B/A QUEST INC | Orlando | P82Z Human Services | $36.3M | 9 |
| PATRIOT SERVICES GROUP INC | Jacksonville | W30 Public & Societal Benefit | $36.2M | 0 |
| MONTVERDE ACADEMY INC | Montverde | — | $36.1M | 7 |
| EARLY LEARNING COALITION OF MANATEE COUNTY INC | Palmetto | B21 Education | $36.1M | 3 |
| COMMUNITY FOUNDATION FOR PALM BEACH AND MARTIN COUNTIES INC | West Palm Beach | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $36.1M | 8 |
| CENTRAL FLORIDA CLINICAL PRACTICE ORGANIZATION INC | Orlando | B11 Education | $36.1M | 15 |
| Habitat for Humanity of Pinellas County Inc DBA Habitat for Humanity | Clearwater | — | $36M | 5 |
| COMMUNITY SCHOOL OF NAPLES INC | Naples | B200 Education | $36M | 7 |
| INTERNATIONAL LONGSHOREMEN'S ASSOCI (AFL-CIO) EMPLOYERS' WELFARE FUND | Hollywood | — | $35.8M | 13 |
| COMMUNITY FOUNDATION OF BROWARD INC | Fort Lauderdale | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $35.8M | 5 |
| Orlando Lutheran Towers Inc | Orlando | L22 Housing & Shelter | $35.7M | 5 |
| CENTRAL FLORIDA REGIONAL WORKFORCE DEVELOPMENT BOARD INC | Orlando | J22 Employment | $35.6M | 12 |
| THE CUSHMAN SCHOOL INC | Miami | B24Z Education | $35.6M | 8 |
| CLINICAL HEALTH NETWORK FOR TRANSFORMATION INC | Miami | E02 Health Care | $35.6M | 1 |
| FLORIDIANS FOR QUALITY VIRTUAL EDUCATION INC | Tampa | B29 Education | $35.6M | 0 |
| THE PORT ROYAL CLUB INC | Naples | — | $35.5M | 12 |
| EARLY LEARNING COALITION OF ST LUCIE COUNTY INC | Ft Pierce | B012 Education | $35.5M | 2 |
| SARASOTA MEMORIAL HEALTHCARE FOUNDATION INC | Sarasota | E22I Health Care | $35.4M | 6 |
| MID FLORIDA AREA AGENCY ON AGING INC | Gainesville | — | $35.4M | 3 |
| INNOVATIONS FINANCIAL CREDIT UNION | Panama City | W61 Public & Societal Benefit | $35.3M | 11 |
| SUNCOAST PACE INC | Lakewood Ranch | P81 Human Services | $35.3M | 4 |
| AVOW Hospice Inc | Naples | P74Z Human Services | $35.2M | 10 |
| Edward Waters University Inc | Jacksonville | B42Z Education | $35.2M | 11 |
| INSTITUTO CENTROAMERICANO DE ADMINISTRACION DE EMPRESAS | Coral Gables | Q99Z International, Foreign Affairs & National Security | $35.1M | 3 |
| MIAMI CEREBRAL PALSY RESIDENTIAL SERVICES INC | Hialeah | — | $35M | 9 |
| MDS FAMILY FOUNDATION INC | Maitland | C34 Environment | $34.9M | 0 |
| South Florida Hotel & Culinary Employee Welfare Fund | Pembroke Pines | — | $34.7M | 0 |
| Good Shepherd Hospice Inc | Temple Terrace | E90 Health Care | $34.7M | 18 |
| Community Supports Inc | Winter Park | P75 Human Services | $34.7M | 1 |
| HEALTH FIRST ADMINISTRATIVE PLANS INC | Rockledge | — | $34.7M | 17 |
| SOUTHWEST FLORIDA RETIREMENT CENTER | Venice | P750 Human Services | $34.6M | 7 |
| NORTON MUSEUM OF ART INC | West Palm Beach | A510 Arts, Culture & Humanities | $34.5M | 7 |
| NEW WORLD SYMPHONY INC | Miami Beach | B40 Education | $34.5M | 11 |
| Ginsburg Family Foundation Inc | Winter Park | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $34.4M | 0 |
| Webber International University | Babson Park | — | $34.4M | 5 |
| SALVADORAN AMERICAN HUMANITARIAN FOUNDATION INC | Miami | Q330 International, Foreign Affairs & National Security | $34.3M | 1 |
| STELLA MARIS HOUSE INC | Lauderdale Lakes | L21Z Housing & Shelter | $34M | 0 |
