| MIAMI CHILDREN'S MUSEUM INC FKA MIAMI YOUTH MUSEUM | Miami | A520 Arts, Culture & Humanities | $10.7M | 4 |
| THE CLASSICAL ACADEMY OF SARASOTA | Sarasota | B29 Education | $10.7M | 1 |
| WAGS PET CLINIC INC | Tampa | D30 Animal-Related | $10.6M | 6 |
| SUWANNEE RIVER ECONOMIC COUNCIL INC | Live Oak | — | $10.6M | 2 |
| OPENDOR MEDIA INC | Sunrise | X300 Religion-Related | $10.6M | 10 |
| GUARDIAN CARE INC | Orlando | E91Z Health Care | $10.6M | 2 |
| FAMILY RESOURCES INC | Pinellas Park | P40Z Human Services | $10.6M | 1 |
| UNITED TEACHERS OF DADE | Miami Springs | — | $10.6M | 30 |
| Urban League of Broward County Inc | Fort Lauderdale | P22Z Human Services | $10.6M | 5 |
| FLORIDA JUSTICE ASSOCIATION | Tallahassee | — | $10.6M | 9 |
| First Coast No More Homeless Pets Inc | Jacksonville | D20 Animal-Related | $10.6M | 5 |
| IMPOWER INC | Longwood | P20Z Human Services | $10.6M | 10 |
| KIDS WISH NETWORK INC | Palm Harbor | P80 Human Services | $10.6M | 1 |
| Hunters Creek Community Assoc | Orlando | L50 Housing & Shelter | $10.5M | 0 |
| PINEAPPLE COVE CLASSICAL ACADEMY AT WEST MELBOURNE INC | West Melbourne | B24 Education | $10.5M | 0 |
| AMB JOHN RAKOLTA JR AND TERRY L RAKOLTA FOUNDATION | Palm Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $10.5M | 0 |
| SUNSHINE STATE ONE-CALL OF FLORIDAINC | Debary | — | $10.5M | 1 |
| SARASOTA SUNCOAST ACADEMY INC | Sarasota | B29 Education | $10.5M | 2 |
| WOMEN IN DISTRESS OF BROWARD COUNTY INC | Lighthouse Point | P43Z Human Services | $10.5M | 4 |
| THE LPGA FOUNDATION | Daytona Beach | B99 Education | $10.5M | 6 |
| OVERTOWN YOUTH CENTER INC | Miami | O20 Youth Development | $10.5M | 2 |
| Alivia Care Inc | Jacksonville | P74 Human Services | $10.5M | 12 |
| VENTUS CHARITABLE FOUNDATION | Palm Beach Gardens | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $10.5M | 0 |
| ST PETERSBURG YACHT CLUB INC | St Petersburg | — | $10.5M | 4 |
| AD IMPACT INC | Sarasota | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $10.5M | 0 |
| EMPLOYU INC | Casselberry | J20 Employment | $10.5M | 8 |
| PARTNERSHIP FOR POLICING INNOVATION AND REFORM | Marathon | I20 Crime & Legal-Related | $10.5M | 7 |
| AMERICAN COLLEGE OF ENDOCRINOLOGY INC | Jacksonville | H00Z Medical Research | $10.5M | 17 |
| BOK TOWER GARDENS INC | Lake Wales | — | $10.5M | 4 |
| Visiting Nurse Association and Hospice Foundation Inc | Vero Beach | E19 Health Care | $10.4M | 2 |
| TSIC INC DBA/TAKE STOCK IN CHILDREN | Fort Lauderdale | I21 Crime & Legal-Related | $10.4M | 3 |
| MORSELIFE FOUNDATION INC | West Palm Beach | P110 Human Services | $10.4M | 6 |
| 4ROOTS FOUNDATION INC | Orlando | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $10.4M | 3 |
| JACKSONVILLE COUNTRY DAY SCHOOL INC | Jacksonville | B21Z Education | $10.4M | 3 |
| BOYS AND GIRLS CLUB OF THE SUNCOAST INC | Clearwater | O23Z Youth Development | $10.4M | 5 |
| EPIC Community Services Inc | St Augustine | F20Z Mental Health & Crisis Intervention | $10.4M | 2 |
| LEGAL AID SERVICE OF BROWARD COUNTY INC | Plantation | — | $10.3M | 6 |
| GROVE XXIII GOLF CLUB INC | Hobe Sound | N6A Recreation & Sports | $10.3M | 3 |
| CITRUS LEVY MARION REGIONAL WORKFORCE DEVELOPMENT BOARD INC | Ocala | J200 Employment | $10.3M | 2 |
| FLORIDA CHAMBER OF COMMERCE INC | Tallahassee | — | $10.3M | 8 |
| GRACE PLACE FOR CHILDREN AND FAMILIES INC | Naples | P20 Human Services | $10.3M | 5 |
| LISA AND STEVEN TANANBAUM FAMILY FOUNDATION | Palm Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $10.3M | 0 |
| THE LOUIS AND GLORIA FLANZER PHILANTHROPIC TRUST C/O Bristol Consult | Sarasota | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $10.3M | 2 |
| Finnish-American Rest Home Inc | Lake Worth | — | $10.3M | 1 |
| SARASOTA BALLET OF FLORIDA INC | Sarasota | A630 Arts, Culture & Humanities | $10.3M | 6 |
| JUAN CARLOS AND UCHI BOTERO AYUDARTE FOUNDATION INC | Miami | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $10.3M | 0 |
| The Edward E Haddock Jr Family Operating Foundation | Orlando | C34 Environment | $10.3M | 0 |
| ORLANDO ECONOMIC PARTNERSHIP INC | Orlando | — | $10.3M | 9 |
| HIGHT IMPACTO INC | Fort Lauderdale | P99 Human Services | $10.2M | 3 |
| MAE VOLEN SENIOR CENTER INC | Boca Raton | — | $10.2M | 5 |
