| GREATER DES MOINES BOTANICAL GARDEN | Des Moines | C114 Environment | $11.8M | 2 |
| North Iowa Community Action Organization | Mason City | — | $11.8M | 2 |
| HUBBELL-WATERMAN FNDN C/O LANE & WATERMAN LLP | Davenport | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $11.8M | 0 |
| FOUNDATION 2 INC | Cedar Rapids | — | $11.8M | 4 |
| OPPORTUNITY LIVING | Lake City | P82Z Human Services | $11.8M | 4 |
| MISSOURI VALLEY LINE CONSTRUCTORS JOINT APPRENTICESHIP AND TRAINING TRUST FUND | Indianola | J21 Employment | $11.7M | 6 |
| Faith Baptist Bible College | Ankeny | B42Z Education | $11.7M | 7 |
| FINANCIAL PLUS CREDIT UNION | West Des Moines | — | $11.6M | 0 |
| The Catholic Foundation for the Archdiocese of Dubuque | Cedar Rapids | X112 Religion-Related | $11.6M | 1 |
| Neighborhood Finance Corporation | Des Moines | L22Z Housing & Shelter | $11.6M | 3 |
| Cottingham & Butler Insurance Services Inc | Dubuque | — | $11.5M | 0 |
| KEOKUK AREA COMMUNITY FOUNDATION | Keokuk | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $11.4M | 0 |
| SIOUXLAND MENTAL HEALTH SERVICES INC | Sioux City | F32Z Mental Health & Crisis Intervention | $11.3M | 6 |
| ST FRANCIS MANOR | Grinnell | — | $11.2M | 7 |
| PLAINS AREA MENTAL HEALTH CENTER I | Le Mars | — | $11.2M | 3 |
| ST LUKE HOMES & SERVICES INC | Spencer | — | $11.1M | 0 |
| HISTORIC ARNOLDS PARK INC | Arnolds Park | A500 Arts, Culture & Humanities | $11.1M | 1 |
| CHILDSERVE COMMUNITY OPTIONS | Johnston | S20Z Community Improvement & Capacity Building | $11.1M | 3 |
| MERCY HOSPITALCEDAR RAPIDSIA ENDOWMENT FOUNDATION INC | Cedar Rapids | E110 Health Care | $11.1M | 5 |
| IOWA ASSOCIATION FOR THE EDUCATION OF YOUNG CHILDREN | West Des Moines | B99Z Education | $11.1M | 1 |
| HOSPICE OF DUBUQUE | Dubuque | — | $11M | 7 |
| REACH FOR YOUR POTENTIAL INC | Iowa City | L21Z Housing & Shelter | $10.9M | 1 |
| INVEST DSM | Des Moines | L25 Housing & Shelter | $10.9M | 3 |
| NORTH STAR COMMUNITY CREDIT UNION | Cherokee | — | $10.8M | 1 |
| AFFINITY CREDIT UNION | Des Moines | — | $10.8M | 4 |
| EMPLOYEES CREDIT UNION | Estherville | — | $10.8M | 3 |
| WALLACE RESEARCH FOUNDATION C/O RSM US LLP ATTN SHAWNA HULS | Cedar Rapids | T20J Philanthropy, Voluntarism & Grantmaking Foundations | $10.7M | 0 |
| EVENTIDE LUTHERAN HOME FOR THE AGED | Denison | E91Z Health Care | $10.7M | 1 |
| The Stanley Foundation dba Stanley Center for Peace and Security | Muscatine | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $10.7M | 5 |
| THE NOBLE FOUNDATION C/O CLAUDIA MIRANDA WEST BANK | West Des Moines | Z99Z Unknown | $10.7M | 0 |
| TABLE TO TABLE FOOD DISTRIBUTION NETWORK | Iowa City | P20 Human Services | $10.7M | 1 |
| NORTH IOWA TRANSITION AND EMPLOYMENT SERVICES INC | Mason City | — | $10.6M | 0 |
| BRAHMANANDA SARASWATI FOUNDATION | Fairfield | X12 Religion-Related | $10.6M | 1 |
| CHARLES E LAKIN HUMAN SERVICES CAMPUS FOUNDATION | Council Bluffs | P11 Human Services | $10.5M | 0 |
| IOWA CONFLUENCE WATER TRAILS | Des Moines | C32 Environment | $10.4M | 1 |
| BETHANY HOME | Dubuque | E910 Health Care | $10.4M | 1 |
| ANIMAL RESCUE LEAGUE OF IOWA INC | Des Moines | D200 Animal-Related | $10.4M | 2 |
| THE UNITED PRESBYTERIAN HOME OF WASHINGTON IOWA | Washington | P750 Human Services | $10.3M | 4 |
| WESCO Industries | Denison | — | $10.3M | 4 |
| GRUNDY COUNTY RURAL ELECTRIC COOPERATIVE | Grundy Center | W80Z Public & Societal Benefit | $10.3M | 8 |
| 1ST GATEWAY CREDIT UNION | Camanche | — | $10.3M | 3 |
| GREATER DES MOINES PARTNERSHIP | Des Moines | S41 Community Improvement & Capacity Building | $10.2M | 11 |
| EARTHMOVING LEGACY CENTER INC | Elkader | A54 Arts, Culture & Humanities | $10.1M | 0 |
| WESLEYLIFE FOUNDATION | Johnston | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $10.1M | 3 |
| Area Residential Care Inc | Dubuque | P75 Human Services | $10M | 2 |
| HOLY SPIRIT RETIREMENT HOME INC | Sioux City | — | $10M | 2 |
| IOWA PUBLIC RADIO INC | Des Moines | A24 Arts, Culture & Humanities | $10M | 3 |
| OSCEOLA ELECTRIC COOPERATIVE INC | Sibley | — | $10M | 12 |
| GRAND VIEW CHRISTIAN SCHOOL ASSOCIATION INC | Des Moines | B99 Education | $9.9M | 2 |
| THE HAROLD W SIEBENS CHARITABLE FOUNDATION INC | Cedar Rapids | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $9.9M | 0 |
