| OSCEOLA MENTAL HEALTH INC | Kissimmee | F32Z Mental Health & Crisis Intervention | $30.1M | 7 |
| ADVENT CHRISTIAN VILLAGE INC | Live Oak | P750 Human Services | $30M | 5 |
| OCHO FOUNDATION | Miami | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $30M | 0 |
| Southeastern Guide Dogs Inc | Palmetto | P860 Human Services | $30M | 9 |
| FISH & WILDLIFE FOUNDATION OF FLORIDA I | Tallahassee | D123 Animal-Related | $30M | 4 |
| OXBRIDGE ACADEMY FOUNDATION INC | West Palm Beach | B11 Education | $29.9M | 10 |
| FLORIDA COLLEGE INC | Temple Terrace | B420 Education | $29.9M | 3 |
| WESLEY MANOR INC | Orlando | P750 Human Services | $29.8M | 8 |
| TAMPA MUSEUM OF ART FOUNDATION INC | Tampa | A115 Arts, Culture & Humanities | $29.7M | 1 |
| AREA AGENCY ON AGING FOR | Fort Myers | — | $29.7M | 2 |
| THE PENINSULA CHARITIES FOUNDATION II | Key Largo | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $29.6M | 0 |
| Big Bend Hospice Inc | Tallahassee | Z99 Unknown | $29.5M | 9 |
| PROJECT HEALTH INC | Sumterville | E39Z Health Care | $29.5M | 13 |
| East Ridge Retirement Village Inc | Gainesville | P750 Human Services | $29.4M | 9 |
| BAY VILLAGE OF SARASOTA INC | Sarasota | P750 Human Services | $29.3M | 8 |
| HOLMES COUNTY HOSPITAL CORPORATION | Bonifay | E22 Health Care | $29.3M | 9 |
| PARC INC | St Petersburg | P820 Human Services | $29.2M | 7 |
| CHAMPIONS FIRST CREDIT UNION | Tallahassee | — | $29M | 9 |
| THE BAKER FAMILY FOUNDATION | Naples | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $29M | 4 |
| IAFF LOCAL 587 HEALTH INSURANCE TRUST FUND | Miami | Y43 Mutual & Membership Benefit | $29M | 5 |
| St Joseph's Enterprises Inc | Tampa | — | $28.9M | 10 |
| CANTERBURY SCHOOL INC | Fort Myers | B200 Education | $28.7M | 5 |
| St Anthony's Professional Buildings & Services Inc | Tampa | — | $28.7M | 10 |
| PGA TOUR FIRST TEE FOUNDATION INC | Ponte Vedra Beach | N6A Recreation & Sports | $28.5M | 7 |
| KLORFINE FOUNDATION | Riviera Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $28.5M | 0 |
| AACSB INTERNATIONAL - THE ASSOCIATION TO ADVANCE COLLEGIATE SCHOOLS OF BUSINESS | Tampa | B51Z Education | $28.5M | 10 |
| TRUE NORTH CLASSICAL ACADEMY INC | Miami | B20 Education | $28.4M | 6 |
| ESCAMBIA COUNTY SCHOOL READINESS COALITION INC | Pensacola | B012 Education | $28.3M | 1 |
| Winston Family Foundation | Jacksonville | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $28.3M | 2 |
| Association of Poinciana Villages | Kissimmee | — | $28.3M | 0 |
| FLORIDA VETERINARY MEDICINE FACULTY ASSOCIATION INC | Gainesville | D034 Animal-Related | $28.2M | 13 |
| THE EUNICE JOYCE GARDINER CHARITABLE FOUNDATION | Palm Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $28.2M | 2 |
| HOSPICE OF THE TREASURE COAST INC | Stuart | — | $28.2M | 11 |
| PLANNED PARENTHOOD OF SOUTH FLORIDA AND THE TREASURE COAST INC | West Palm Beach | E42 Health Care | $28.2M | 10 |
| Tampa Firefighters and Police Officers Employees Health Plan | Pembroke Pines | Y43 Mutual & Membership Benefit | $28.1M | 0 |
| SOUTHWEST FLORIDA COMMUNITY FOUNDATION INC | Fort Myers | T31Z Philanthropy, Voluntarism & Grantmaking Foundations | $28.1M | 5 |
| PINES OF SARASOTA HEALTHCARE LLC | Sarasota | P20 Human Services | $28.1M | 10 |
| YOUTH AND FAMILY ALTERNATIVES INC | New Port Richey | P400 Human Services | $28.1M | 2 |
| FLORIDA GULF COAST UNIVERSITY FOUNDATION INC | Fort Myers | B110 Education | $27.9M | 6 |
| HIS HOUSE INC | Opa Locka | — | $27.8M | 7 |
| AVANT GARDE ACADEMY FOUNDATION | Hollywood | B11 Education | $27.7M | 3 |
| Lightshare Behavioral Wellness & Recovery Inc | Sarasota | F220 Mental Health & Crisis Intervention | $27.5M | 5 |
| St Joseph's Health Care Center Inc | Tampa | E210 Health Care | $27.5M | 10 |
| TRI-COUNTY HUMAN SERVICES INC | Lakeland | F210 Mental Health & Crisis Intervention | $27.5M | 8 |
| UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION INC | Orlando | T22Z Philanthropy, Voluntarism & Grantmaking Foundations | $27.5M | 4 |
| THE HEBREW DAY SCHOOL OF BROWARD COUNTY | Davie | — | $27.5M | 7 |
| THE NATIONAL CENTER FOR CONSTRUCTION EDU AND RESEARCH LTD | Alachua | J220 Employment | $27.5M | 9 |
| STEP UP FOR STUDENTS INC | Jacksonville | P20 Human Services | $27.4M | 13 |
| NELSON & CLAUDIA PELTZ FAMILY FOUNDATION | Palm Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $27.4M | 0 |
| KIPP MIAMI INC | Miami | B29 Education | $27.4M | 3 |
