| Oneota Riverview Care Facility Inc | Decorah | F33Z Mental Health & Crisis Intervention | $6.8M | 2 |
| The Wellmark Foundation | Des Moines | — | $6.8M | 0 |
| FOOD BANK OF SIOUXLAND INC | Sioux City | — | $6.8M | 1 |
| COMMUNITY HOUSING INITIATIVES INC | Spencer | L200 Housing & Shelter | $6.7M | 2 |
| NATIONAL FORENSIC LEAGUE | West Des Moines | A700 Arts, Culture & Humanities | $6.7M | 5 |
| DUBUQUE COMMUNITY Y | Dubuque | P27 Human Services | $6.7M | 2 |
| UNITED WAY QUAD CITIES | Bettendorf | T70Z Philanthropy, Voluntarism & Grantmaking Foundations | $6.7M | 3 |
| Exceptional Opportunities Inc | Algona | F33Z Mental Health & Crisis Intervention | $6.6M | 1 |
| HOSPICE OF SIOUXLAND | Sioux City | E92 Health Care | $6.6M | 6 |
| UNITED COMMUNITY HEALTH CENTER | Storm Lake | E32 Health Care | $6.6M | 3 |
| WORTH COUNTY DEVELOPMENT AUTHORITY | Northwood | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $6.6M | 1 |
| HOYT SHERMAN PLACE FOUNDATION | Des Moines | A80 Arts, Culture & Humanities | $6.5M | 1 |
| CATHOLIC COUNCIL FOR SOCIAL CONCERN INC | Des Moines | — | $6.5M | 1 |
| DES MOINES METRO OPERA INC | Indianola | A6A0 Arts, Culture & Humanities | $6.4M | 1 |
| CONSERVATION FOUNDATION of DICKINSON COUNTY | Okoboji | C30 Environment | $6.4M | 0 |
| DEWOLF FAMILY FOUNDATION | Cedar Rapids | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $6.4M | 0 |
| IOWA CONSTRUCTION CAREER INSTITUTE | West Des Moines | Z99 Unknown | $6.4M | 2 |
| PEOPLES CREDIT UNION | Webster City | — | $6.3M | 2 |
| PELLA CHRISTIAN GRADE SCHOOL | Pella | — | $6.3M | 0 |
| MEDIC EMS | Davenport | — | $6.3M | 15 |
| DELTA DENTAL OF IOWA FOUNDATION | Johnston | E70 Health Care | $6.2M | 11 |
| VARIETY - THE CHILDREN'S CHARITY OF IOWA | Des Moines | — | $6.2M | 1 |
| WAKONDA CLUB | Des Moines | — | $6.2M | 4 |
| SIOUXLAND COMMUNITY FOUNDATION | Sioux City | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $6.2M | 1 |
| CALHOUN COUNTY ELECTRIC COOPERATIVE | Rockwell City | — | $6.2M | 11 |
| COMMUNITY MEMORIAL HEALTH CENTER | Hartley | — | $6.1M | 5 |
| SHELBY COUNTY MEDICAL CORPORATION | Harlan | E11 Health Care | $6.1M | 7 |
| AGING RESOURCES OF CENTRAL IOWA | Des Moines | P81Z Human Services | $6.1M | 2 |
| SALEM LUTHERAN HOMES INC | Elk Horn | — | $6.1M | 0 |
| IOWA ASSOCIATION OF ELECTRIC | Des Moines | — | $6.1M | 13 |
| ON WITH LIFE EXTENDED SERVICES LTD | Ankeny | E91 Health Care | $6.1M | 6 |
| MAHARISHI FOUNDATION INTERNATIONAL | Fairfield | P20 Human Services | $6.1M | 5 |
| JENSEN CONSTRUCTION COMPANY GROUP TRUST | Des Moines | — | $6.1M | 0 |
| IN CONCERTCARE INC | Des Moines | E82B Health Care | $6M | 7 |
| FAITH LUTHERAN HOME | Osage | — | $6M | 1 |
| SEED SAVERS EXCHANGE INC | Decorah | K200 Food, Agriculture & Nutrition | $6M | 3 |
| OCHYLSKI FAMILY FOUNDATION | Des Moines | T22Z Philanthropy, Voluntarism & Grantmaking Foundations | $6M | 0 |
| United Food & Commercial Workers Union Local 222IN | Sioux City | — | $5.9M | 31 |
| PEO Program for Continuing Education | Des Moines | — | $5.9M | 0 |
| MIDLAND CREDIT UNION | Urbandale | — | $5.9M | 2 |
| GIRL SCOUTS OF EASTERN IOWA AND WESTERN | Bettendorf | — | $5.9M | 3 |
| STOEL RIVES VEBA TRUST | Des Moines | Y43 Mutual & Membership Benefit | $5.9M | 1 |
| EMPLOYEE & FAMILY RESOURCES INC | Des Moines | — | $5.9M | 3 |
| Elderbridge Agency on Aging | Mason City | P81Z Human Services | $5.9M | 2 |
| RIVER BEND TRANSIT | Davenport | W40Z Public & Societal Benefit | $5.8M | 5 |
| CORNERSTONE SENIOR COMMUNITIES | Sutherland | P75 Human Services | $5.8M | 1 |
| THE HALL-PERRINE FOUNDATION | Cedar Rapids | — | $5.8M | 2 |
| PEO Educational Loan Fund | Des Moines | B82 Education | $5.8M | 0 |
| CLARENCE NURSING HOME INC | Clarence | E91Z Health Care | $5.8M | 1 |
| PRAIRIE VIEW MANAGEMENT INC | Hawkeye | F33 Mental Health & Crisis Intervention | $5.8M | 1 |
