| THE YOUNG MEN'S CHRISTIAN ASSOCIATION OF THE CEDAR RAPIDS METROPOLITAN AREA | Cedar Rapids | P27Z Human Services | $6.9M | 2 |
| Greater Poweshiek Community Foundation | Grinnell | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $6.8M | 2 |
| ELMCREST COUNTRY CLUB | Cedar Rapids | — | $6.8M | 1 |
| AEGON TRANSAMERICA FOUNDATION | Cedar Rapids | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $6.8M | 0 |
| World Food Prize Foundation | Urbandale | T23 Philanthropy, Voluntarism & Grantmaking Foundations | $6.8M | 2 |
| DAVENPORT COUNTRY CLUB | Pleasant Valley | — | $6.8M | 4 |
| BAUM HARMON MERCY HOSPITAL | Sioux City | E220 Health Care | $6.8M | 10 |
| COMMUNITY HOUSING INITIATIVES INC | Spencer | L200 Housing & Shelter | $6.7M | 2 |
| NATIONAL FORENSIC LEAGUE D/B/A NATIONAL SPEECH AND DEBATE ASSOC | West Des Moines | A700 Arts, Culture & Humanities | $6.7M | 5 |
| DUBUQUE COMMUNITY Y | Dubuque | P27 Human Services | $6.7M | 2 |
| UNITED WAY QUAD CITIES | Bettendorf | T70Z Philanthropy, Voluntarism & Grantmaking Foundations | $6.7M | 3 |
| Exceptional Opportunities Inc | Algona | F33Z Mental Health & Crisis Intervention | $6.6M | 1 |
| UNITED COMMUNITY HEALTH CENTER | Storm Lake | E32 Health Care | $6.6M | 3 |
| WORTH COUNTY DEVELOPMENT AUTHORITY | Northwood | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $6.6M | 1 |
| AFFORDABLE HOUSING NETWORK INC | Cedar Rapids | L20 Housing & Shelter | $6.5M | 4 |
| HOYT SHERMAN PLACE FOUNDATION | Des Moines | A80 Arts, Culture & Humanities | $6.5M | 1 |
| BLACK HAWK-GRUNDY MENTAL HEALTH CENTER INC | Waterloo | F32 Mental Health & Crisis Intervention | $6.5M | 5 |
| DUBUQUE SYMPHONY ORCHESTRA | Dubuque | — | $6.5M | 1 |
| CATHOLIC COUNCIL FOR SOCIAL CONCERN INC CATHOLIC CHARITIES | Des Moines | — | $6.5M | 1 |
| ELLIPSIS INC | Johnston | F33Z Mental Health & Crisis Intervention | $6.4M | 3 |
| DES MOINES METRO OPERA INC | Indianola | A6A0 Arts, Culture & Humanities | $6.4M | 1 |
| CONSERVATION FOUNDATION of DICKINSON COUNTY | Okoboji | C30 Environment | $6.4M | 0 |
| MAKE-A-WISH FOUNDATION OF IOWA INC | Urbandale | E86Z Health Care | $6.4M | 1 |
| UNIVERSITY OF IOWA RESEARCH FOUNDATION | Iowa City | — | $6.4M | 11 |
| GENESIS HEALTH SERVICES FOUNDATION | Davenport | E110 Health Care | $6.4M | 5 |
| SERVE CREDIT UNION | Des Moines | — | $6.4M | 0 |
| IOWA CONSTRUCTION CAREER INSTITUTE | West Des Moines | Z99 Unknown | $6.4M | 2 |
| THE BUTLER FAMILY FOUNDATION | Dubuque | T99Z Philanthropy, Voluntarism & Grantmaking Foundations | $6.3M | 0 |
| UNITED WAY OF EAST CENTRAL IOWA | Cedar Rapids | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $6.3M | 2 |
| HOSPICE OF SIOUXLAND | Sioux City | E92 Health Care | $6.3M | 6 |
| PEOPLES CREDIT UNION | Webster City | — | $6.3M | 2 |
| PELLA CHRISTIAN GRADE SCHOOL | Pella | — | $6.3M | 0 |
| MEDIC EMS | Davenport | — | $6.3M | 15 |
| DELTA DENTAL OF IOWA FOUNDATION | Johnston | E70 Health Care | $6.2M | 11 |
| VARIETY - THE CHILDREN'S CHARITY OF IOWA | Des Moines | — | $6.2M | 1 |
| WAKONDA CLUB | Des Moines | — | $6.2M | 4 |
| SIOUXLAND COMMUNITY FOUNDATION | Sioux City | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $6.2M | 1 |
| CALHOUN COUNTY ELECTRIC COOPERATIVE ASSOCIATION | Rockwell City | — | $6.2M | 11 |
| HILLCREST HOME | Sumner | — | $6.1M | 1 |
| COMMUNITY MEMORIAL HEALTH CENTER | Hartley | — | $6.1M | 5 |
| SALEM LUTHERAN HOMES INC | Elk Horn | — | $6.1M | 0 |
| IOWA ASSOCIATION OF ELECTRIC COOPERATIVES | Des Moines | — | $6.1M | 13 |
| AGING RESOURCES OF CENTRAL IOWA | Des Moines | P81Z Human Services | $6.1M | 2 |
| THE BUTLER CHILDREN'S GARDEN FOUNDATION INC | Dubuque | T23 Philanthropy, Voluntarism & Grantmaking Foundations | $6.1M | 0 |
| MAHARISHI FOUNDATION INTERNATIONAL | Fairfield | P20 Human Services | $6.1M | 5 |
| JENSEN CONSTRUCTION COMPANY GROUP TRUST | Des Moines | — | $6.1M | 0 |
| SEED SAVERS EXCHANGE INC | Decorah | K200 Food, Agriculture & Nutrition | $6M | 3 |
| FAITH LUTHERAN HOME | Osage | — | $6M | 1 |
| OCHYLSKI FAMILY FOUNDATION | Des Moines | T22Z Philanthropy, Voluntarism & Grantmaking Foundations | $6M | 0 |
| United Food & Commercial Workers Union Local 222IN | Sioux City | — | $5.9M | 31 |
