| THE VOTER PARTICIPATION CENTER | Washington | R40 Civil Rights, Social Action & Advocacy | $22.7M | 8 |
| Hudson Institute Inc | Washington | — | $22.7M | 13 |
| THE FEDERALIST SOCIETY FOR LAW AND | Washington | — | $22.5M | 11 |
| iMMAP Inc | Washington | Q30 International, Foreign Affairs & National Security | $22.4M | 4 |
| THE PERSONAL CARE PRODUCTS COUNCIL INC | Washington | — | $22.4M | 14 |
| UNITED WE DREAM NETWORK INC | Washington | R20 Civil Rights, Social Action & Advocacy | $22.4M | 7 |
| HOUSE MAJORITY FORWARD | Washington | S01 Community Improvement & Capacity Building | $22.3M | 7 |
| FOOD & WATER WATCH | Washington | K05 Food, Agriculture & Nutrition | $22.3M | 6 |
| ASSOCIATION FOR ACCESSIBLE MEDICINES | Washington | S41 Community Improvement & Capacity Building | $22.2M | 9 |
| INFORMATION TECHNOLOGY INDUSTRY COUNCIL | Washington | — | $22.2M | 12 |
| WOODROW WILSON INTERNATIONAL CENTER | Washington | V350 Social Science | $22.1M | 10 |
| Girl Scout Council of the Nation's | Washington | O42Z Youth Development | $22.1M | 5 |
| Family Research Council | Washington | P01 Human Services | $22M | 9 |
| APPLETREE INSTITUTE FOR EDUCATION | Washington | B99Z Education | $21.9M | 12 |
| Physicians Committee for Responsible Med | Washington | E85Z Health Care | $21.9M | 6 |
| MEDIA MATTERS FOR AMERICA | Washington | B99 Education | $21.9M | 14 |
| AMERICAN RIVERS INC | Washington | C013 Environment | $21.8M | 11 |
| EUGENE & AGNES E MEYER FOUNDATION | Washington | — | $21.8M | 2 |
| UFCW HEALTH INSURANCE PLAN FOR RETIREES | Washington | Y43 Mutual & Membership Benefit | $21.8M | 6 |
| SMART GROUP VSTD PLAN AND TRUST | Washington | Y43 Mutual & Membership Benefit | $21.8M | 4 |
| ENVIRONMENTAL WORKING GROUP | Washington | C35 Environment | $21.8M | 9 |
| SCORE Association | Washington | — | $21.7M | 11 |
| THE GLOBAL DEVELOPMENT INCUBATOR INC | Washington | Q32 International, Foreign Affairs & National Security | $21.5M | 8 |
| ATLANTIC 10 CONFERENCE | Washington | N400 Recreation & Sports | $21.5M | 6 |
| MERIDIAN PUBLIC CHARTER SCHOOL | Washington | B20 Education | $21.5M | 6 |
| The National Restaurant Association | Washington | K99E Food, Agriculture & Nutrition | $21.4M | 12 |
| HUMAN RIGHTS CAMPAIGN FOUNDATION | Washington | R260 Civil Rights, Social Action & Advocacy | $21.4M | 0 |
| Advanced Energy Institute | Washington | C20 Environment | $21.3M | 8 |
| BANCROFT GLOBAL DEVELOPMENT | Washington | Q41 International, Foreign Affairs & National Security | $21.3M | 0 |
| NORTH AMERICAN REGION OF THE AIRPORTS | Washington | — | $21.3M | 9 |
| NATIONAL LEAGUE OF CITIES INSTITUTE INC | Washington | B11 Education | $21.3M | 7 |
| THE FUND FOR AMERICAN STUDIES | Washington | — | $21.2M | 9 |
| NATIONAL EDUCATION ASSOCIATION AND | Washington | Y43 Mutual & Membership Benefit | $21.1M | 3 |
| THE WASHINGTON CENTER FOR INTERNSHIPS & | Washington | — | $21.1M | 8 |
| FUTURE NOW ACTION | Washington | W05 Public & Societal Benefit | $21M | 6 |
| UAW RETIREES OF THE BUDD COMPANY HEALTH | Washington | Y43 Mutual & Membership Benefit | $20.9M | 5 |
| MILTON GOTTESMAN JEWISH DAY SCHOOL OF | Washington | B20 Education | $20.9M | 7 |
| NATIONAL ASSOCIATION FOR THE EDUCATION | Washington | B030 Education | $20.8M | 10 |
| HEART RHYTHM SOCIETY INC | Washington | — | $20.8M | 14 |
| HILLWOOD ESTATE MUSEUM & GARDENS | Washington | A80 Arts, Culture & Humanities | $20.8M | 8 |
| Friendship Place | Washington | L41 Housing & Shelter | $20.7M | 7 |
| COLORECTAL CANCER ALLIANCE | Washington | E60 Health Care | $20.7M | 13 |
| CENTRAL UNION MISSION | Washington | L410 Housing & Shelter | $20.7M | 3 |
| PROGRESSIVE LIFE CENTER INC | Washington | — | $20.7M | 0 |
| PLANNED PARENTHOOD OF METROPOLITAN | Washington | E420 Health Care | $20.7M | 13 |
| THE FIELD SCHOOL INC | Washington | — | $20.6M | 8 |
| AMERICAN ASSOCIATION OF COLLEGES OF | Washington | B030 Education | $20.6M | 8 |
| PBM ACCOUNTABILITY PROJECT | Washington | — | $20.4M | 0 |
| JACK & JILL OF AMERICA INC | Washington | — | $20.4M | 0 |
| PROSPERITY NOW | Washington | S31Z Community Improvement & Capacity Building | $20.4M | 9 |
