| WASHINGTON CITY ORPHAN ASYLUM | Washington | — | $13.8M | 5 |
| Native Americans in Philanthropy | Washington | R99 Civil Rights, Social Action & Advocacy | $13.8M | 3 |
| AMERICAN COUNCIL FOR AN ENERGY-EFFICIENT | Washington | C013 Environment | $13.8M | 8 |
| ASIAN AND PACIFIC ISLANDER AMERICAN | Washington | B82 Education | $13.7M | 7 |
| National Coalition for Asian Pacific | Washington | S21 Community Improvement & Capacity Building | $13.7M | 6 |
| THE PHILLIPS COLLECTION | Washington | A510 Arts, Culture & Humanities | $13.7M | 9 |
| AMERICANS UNITED FOR SEPARATION OF | Washington | R40 Civil Rights, Social Action & Advocacy | $13.7M | 10 |
| THE MANAGEMENT CENTER | Washington | B99 Education | $13.6M | 12 |
| CAPITAL AREA IMMIGRANTS' RIGHTS | Washington | I83 Crime & Legal-Related | $13.6M | 6 |
| THE CENTER FOR ADVANCED DEFENSE STUDIES | Washington | Q40 International, Foreign Affairs & National Security | $13.6M | 12 |
| CHIEFS FOR CHANGE | Washington | B90 Education | $13.6M | 7 |
| DESTINATIONS INTERNATIONAL ASSOCIATION | Washington | — | $13.5M | 15 |
| MONUMENT ACADEMY INC | Washington | B29 Education | $13.5M | 9 |
| THE ARC OF THE UNITED STATES | Washington | P82 Human Services | $13.5M | 8 |
| FRIENDS OF THE OSA | Washington | C30 Environment | $13.4M | 2 |
| BARBARA CHAMBERS CHILDREN CENTER | Washington | P330 Human Services | $13.4M | 0 |
| COMMON CAUSE EDUCATION FUND | Washington | W20 Public & Societal Benefit | $13.4M | 7 |
| LEE MONTESSORI PUBLIC CHARTER SCHOOL | Washington | B29 Education | $13.4M | 6 |
| THE FARM CREDIT COUNCIL | Washington | — | $13.3M | 37 |
| UFCW HEALTH INSURANCE PLAN FOR | Washington | Y43 Mutual & Membership Benefit | $13.3M | 6 |
| THE LEADERSHIP CONFERENCE | Washington | W70Z Public & Societal Benefit | $13.3M | 13 |
| National Governors Association Center for Best Practices | Washington | — | $13.3M | 13 |
| THE LINKS FOUNDATION INCORPORATED | Washington | — | $13.3M | 1 |
| CENTER FOR APPLIED LINGUISTICS | Washington | — | $13.3M | 7 |
| Stoddard Baptist Home | Washington | Z99Z Unknown | $13.2M | 7 |
| COUNCIL ON FOUNDATIONS INC | Washington | T500 Philanthropy, Voluntarism & Grantmaking Foundations | $13.2M | 11 |
| The River School | Washington | B28 Education | $13.2M | 7 |
| KINGSMAN ACADEMY PUBLIC CHARTER SCHOOL | Washington | B20 Education | $13.2M | 6 |
| UNITED STATES ENERGY ASSOCIATION INC | Washington | Q30Z International, Foreign Affairs & National Security | $13.2M | 10 |
| FAMILY AND MEDICAL COUNSELING | Washington | F21Z Mental Health & Crisis Intervention | $13.2M | 9 |
| INTERNATIONAL CENTER FOR NOT-FOR-PROFIT | Washington | Q300 International, Foreign Affairs & National Security | $13.1M | 10 |
| AMERICAN SOCIETY OF LANDSCAPE ARCHITECTS | Washington | — | $13.1M | 9 |
| TANAGER | Washington | Q310 International, Foreign Affairs & National Security | $13.1M | 8 |
| SUSTAINABLE FORESTRY INITIATIVE INC | Washington | C36 Environment | $13.1M | 8 |
| THE HENRY L STIMSON CENTER | Washington | Q054 International, Foreign Affairs & National Security | $13M | 9 |
| NATIONAL HOUSING TRUST | Washington | L20Z Housing & Shelter | $13M | 9 |
| BIOONE | Washington | U40 Science & Technology | $12.9M | 4 |
| BUSINESS GROUP ON HEALTH | Washington | E00F Health Care | $12.9M | 8 |
| THE NEXT STEP PUBLIC CHARTER | Washington | B20 Education | $12.9M | 10 |
| SHERIDAN SCHOOL | Washington | B24Z Education | $12.8M | 8 |
| CONGRESSIONAL HISPANIC CAUCUS | Washington | — | $12.8M | 7 |
| THE INSTITUTE OF WORLD POLITICS | Washington | Q055 International, Foreign Affairs & National Security | $12.8M | 14 |
| INSTITUTE FOR STRATEGIC DIALOGUE-US | Washington | B90 Education | $12.8M | 7 |
| SANT CHARITABLE FOUNDATION INC | Washington | — | $12.8M | 2 |
| READING IS FUNDAMENTAL INC | Washington | B92Z Education | $12.8M | 7 |
| AMERICAN FINANCIAL SERVICES ASSOCIATION | Washington | J40 Employment | $12.8M | 12 |
| AMERICAN EDUCATIONAL RESEARCH | Washington | B00E Education | $12.8M | 7 |
| GIFFORDS | Washington | S80 Community Improvement & Capacity Building | $12.8M | 10 |
| BREAKTHROUGH PRIZE IN LIFE SCIENCES | Washington | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $12.7M | 2 |
| CESAR CHAVEZ PUBLIC CHARTER SCHOOL | Washington | B25Z Education | $12.7M | 4 |
