| Naples Botanical Garden Inc | Naples | C410 Environment | $27.4M | 8 |
| ST JOHN'S REHABILITATION HOSPITAL AND NURSING CENTER INC | Lauderdale Lakes | E50 Health Care | $27.3M | 9 |
| Restoration Bridge International Inc | West Palm Beach | P20 Human Services | $27.3M | 1 |
| Discover Palm Beach Inc | West Palm Beach | — | $27.3M | 5 |
| RIVER GARDEN HEBREW HOME FOR THE AGED INC | Jacksonville | E910 Health Care | $27M | 9 |
| The United Way of Lee County Inc | Fort Myers | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $27M | 4 |
| RUTH & NORMAN RALES JEWISH FAMILY SERVICES INC | Boca Raton | F60 Mental Health & Crisis Intervention | $26.8M | 5 |
| KINGS POINT RECREATION CORPORATION | Delray Beach | N50 Recreation & Sports | $26.8M | 0 |
| SOUTH COUNTY MENTAL HEALTH CENTER INC | Delray Beach | F320 Mental Health & Crisis Intervention | $26.7M | 6 |
| Habitat for Humanity for Lee and Hendry Counties Inc | Fort Myers | L20Z Housing & Shelter | $26.6M | 3 |
| GLOBAL LEADERSHIP ACADEMY INC | Jacksonville | B29 Education | $26.4M | 2 |
| ALFRED B MACLAY JR DAY SCHOOL | Tallahassee | — | $26.3M | 3 |
| HELPING HANDS MINISTRIES INC | Sarasota | P20 Human Services | $26.2M | 1 |
| JEWISH LEADERSHIP ACADEMY INC | Miami | B28 Education | $26.2M | 9 |
| FLORIDA BANKERS HEALTH CONSORTIUM INC | Winter Park | Y34 Mutual & Membership Benefit | $26.2M | 0 |
| LAWFARE DEFENSE FUND INC | Venice | I12 Crime & Legal-Related | $26.1M | 0 |
| Ahavat Habriah Incorporated | Fort Lauderdale | X30 Religion-Related | $26.1M | 0 |
| LATINOS SALUD INC | Wilton Manors | B99 Education | $26M | 7 |
| JEWISH FEDERATION OF SOUTH PALM BEACH COUNTY INC | Boca Raton | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $26M | 7 |
| UNITED WAY OF NORTHEAST FLORIDA INC | Jacksonville | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $26M | 8 |
| THE WILLIAM B SNYDER CHARITABLE TRUST DATED 08/12/2020 | Clearwater | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $25.9M | 0 |
| TAMPA PREPARATORY SCHOOL INC | Tampa | B250 Education | $25.9M | 5 |
| PALM BEACH DAY ACADEMY INC | Palm Beach | B24Z Education | $25.8M | 4 |
| ST MARK VILLAGE INC | Palm Harbor | P750 Human Services | $25.7M | 7 |
| LAKELAND CHRISTIAN SCHOOL INC | Lakeland | B24Z Education | $25.7M | 3 |
| DONNA KLEIN JEWISH ACADEMY INC | Boca Raton | B20 Education | $25.7M | 6 |
| GivenGain Foundation USA | Fort Lauderdale | T50 Philanthropy, Voluntarism & Grantmaking Foundations | $25.7M | 1 |
| THE CONSORTIUM OF FLORIDA EDUCATION FOUNDATIONS INC | Gainesville | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $25.6M | 1 |
| FEEDING FLORIDA INC | Tallahassee | — | $25.6M | 1 |
| USF FINANCING CORPORATION | Tampa | B900 Education | $25.6M | 6 |
| PROJECT ACCESS FOUNDATION INC | Miami | B90 Education | $25.5M | 8 |
| SUNCOAST MANOR RETIREMENT COMMUNITY INC | Orlando | — | $25.4M | 8 |
| CHILDREN'S FORUM INC | Tallahassee | P330 Human Services | $25.2M | 7 |
| CHILDREN'S DIAGNOSTIC & TREATMENT CENTER INC | Fort Lauderdale | E300 Health Care | $25.1M | 8 |
| COVENANT LIVING OF FLORIDA INC | Plantation | P750 Human Services | $25.1M | 18 |
| Tree of Knowledge Learning Academy Inc | Miami Beach | B999 Education | $25M | 0 |
| C R MOORE LEGACY FOUNDATION INC | Lk Worth Bch | L22Z Housing & Shelter | $25M | 2 |
| CARRFOUR SUPPORTIVE HOUSING INC | Miami | L99Z Housing & Shelter | $25M | 5 |
| UNITED JEWISH COMMUNITY OF BROWARD COUNTY INC | Davie | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $24.9M | 8 |
| THE CRISIS CENTER OF TAMPA BAY INC | Tampa | F400 Mental Health & Crisis Intervention | $24.8M | 6 |
| R'CLUB CHILD CARE INC | St Petersburg | — | $24.8M | 4 |
| GATEWAY COMMUNITY SERVICES INC | Jacksonville | F12 Mental Health & Crisis Intervention | $24.8M | 2 |
| SOUTH FLORIDA FAIR & PALM BEACH COUNTY EXPOSITIONS INC | West Palm Beach | N520 Recreation & Sports | $24.8M | 7 |
| EASTER SEALS FLORIDA INC | Winter Park | E50Z Health Care | $24.8M | 4 |
| Greater Miami Hebrew Academy | Miami Beach | X30Z Religion-Related | $24.7M | 3 |
| FLORIDA ASSOCIATION OF COURT CLERKS | Tallahassee | — | $24.7M | 11 |
| IM SULZBACHER CENTER FOR THE HOMELESS INC | Jacksonville | L20 Housing & Shelter | $24.6M | 5 |
| EARLY LEARNING COALITION OF LAKE COUNTY INC | Leesburg | B012 Education | $24.6M | 1 |
| HANLEY CENTER FOUNDATION INC | West Palm Beach | F20 Mental Health & Crisis Intervention | $24.6M | 11 |
| Easter Seals Southwest Florida Inc | Sarasota | E50 Health Care | $24.6M | 6 |
