| University of Miami | Coral Gables | B430 Education | $6.5B | 17 |
| Adventist Health SystemSunbelt Inc | Altamonte Springs | E210 Health Care | $6.3B | 25 |
| Orlando Health Inc | Orlando | E210 Health Care | $4.8B | 21 |
| FLORIDA HEALTH SCIENCES CENTER INC | Tampa | E220 Health Care | $3.2B | 18 |
| Baptist Hospital of Miami Inc | Miami | E220 Health Care | $2.6B | 14 |
| Southern Baptist Hospital of Florida Inc | Jacksonville | E220 Health Care | $2.6B | 11 |
| SHANDS TEACHING HOSPITAL AND CLINICS INC | Gainesville | E220 Health Care | $2.3B | 37 |
| THE NEMOURS FOUNDATION | Jacksonville | E240 Health Care | $2.1B | 43 |
| H LEE MOFFITT CANCER CENTER AND | Tampa | E240 Health Care | $2B | 17 |
| St Joseph's Hospital Inc | Tampa | E220 Health Care | $2B | 15 |
| SHRINERS HOSPITALS FOR CHILDREN | Tampa | E240 Health Care | $1.8B | 9 |
| Adventist Health System Sunbelt | Altamonte Springs | E210 Health Care | $1.6B | 49 |
| Baptist Health South Florida Inc | Coral Gables | E210 Health Care | $1.3B | 24 |
| Lakeland Regional Medical Center Inc | Lakeland | E220 Health Care | $1.3B | 22 |
| University Community Hospital Inc | Tampa | E220 Health Care | $1.1B | 37 |
| SUNCOAST CREDIT UNION | Tampa | W61 Public & Societal Benefit | $1.1B | 23 |
| NCF CHARITABLE TRUST | Clearwater | X11 Religion-Related | $1.1B | 0 |
| CAPITAL HEALTH PLAN INC | Tallahassee | — | $1.1B | 29 |
| TALLAHASSEE MEMORIAL HEALTHCARE INC | Tallahassee | E220 Health Care | $1.1B | 11 |
| Orlando Health Medical Group Inc | Orlando | E210 Health Care | $1.1B | 11 |
| MOUNT SINAI MEDICAL CENTER OF | Miami Beach | E220 Health Care | $1.1B | 17 |
| Florida Clinical Practice Association | Gainesville | — | $1.1B | 41 |
| FLORIDA HOSPITAL MEDICAL GROUP INC | Maitland | — | $1B | 18 |
| BayCare Health System Inc | Clearwater | E210 Health Care | $1B | 13 |
| Morton Plant Hospital Association Inc | Clearwater | E220 Health Care | $1B | 14 |
| Variety Children's Hospital | Miami | E240 Health Care | $974.6M | 14 |
| SOUTH MIAMI HOSPITAL INC | Miami | E220 Health Care | $941.6M | 12 |
| Baptist Health Care Inc | Pensacola | E220 Health Care | $939.6M | 29 |
| Nova Southeastern University Inc | Fort Lauderdale | B430 Education | $937.8M | 14 |
| SHANDS JACKSONVILLE MEDICAL CENTER INC | Jacksonville | E220 Health Care | $916.1M | 27 |
| NAPLES COMMUNITY HOSPITAL INC | Naples | E220 Health Care | $880.7M | 25 |
| HOLMES REGIONAL MEDICAL CENTER INC | Rockledge | E220 Health Care | $864.6M | 11 |
| Boca Raton Regional Hospital Inc | Boca Raton | E220 Health Care | $837.2M | 13 |
| DECHOMAI ASSET TRUST | Jacksonville | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $837M | 0 |
| PGA TOUR INC | Ponte Vedra Beach | — | $760.6M | 26 |
| VyStar Credit Union | Jacksonville | — | $759.7M | 19 |
| Baptist Health Medical Group Inc | Coral Gables | E21 Health Care | $756.4M | 11 |
| EMBRY-RIDDLE AERONAUTICAL UNIVERSITY INC | Daytona Beach | B430 Education | $703M | 19 |
| West Kendall Baptist Hospital Inc | Miami | E20 Health Care | $683.7M | 11 |
| MEMORIAL HEALTH SYSTEMS INC | Daytona Beach | E20 Health Care | $665.9M | 33 |
| HOLY CROSS HOSPITAL INC | Fort Lauderdale | E220 Health Care | $632.6M | 18 |
| EVERGLADES COLLEGE INC | Ft Lauderdale | B42 Education | $629.4M | 20 |
| LEE COUNTY ELECTRIC COOPERATIVE INC | North Fort Myers | — | $616.1M | 24 |
| RAYMOND JAMES CHARITABLE ENDOWMENT FUND | St Petersburg | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $580.4M | 5 |
| WITHLACOOCHEE RIVER ELECTRIC | Dade City | — | $574.8M | 17 |
| Trustees of Mease Hospital Inc | Dunedin | E220 Health Care | $572.2M | 12 |
| BayCare Medical Group Inc | Tampa | E32Z Health Care | $559.9M | 20 |
| Florida Hospital Ocala Inc | Ocala | — | $558.9M | 25 |
| St Anthony's Hospital Inc | St Petersburg | E220 Health Care | $557.8M | 11 |
| Homestead Hospital Inc | Homestead | E22 Health Care | $529.9M | 10 |
