| UNIVERSITY OF ALABAMA HEALTH SERVICES FOUNDATION PC | Birmingham | E60 Health Care | $1.2B | 32 |
| The Children's Hospital of Alabama | Birmingham | E240 Health Care | $953.2M | 12 |
| PowerSouth Energy Cooperative | Andalusia | — | $859.7M | 60 |
| SOUTHEASTERN CONFERENCE | Birmingham | N700 Recreation & Sports | $839.7M | 6 |
| NAVIGATE AFFORDABLE HOUSING PARTNERS INC | Birmingham | L20Z Housing & Shelter | $834.3M | 7 |
| MOBILE INFIRMARY ASSOCIATION | Mobile | E220 Health Care | $654.6M | 15 |
| GULF HEALTH HOSPITALS INC DBA THOMAS HOSPITAL NORTH BALDWIN | Mobile | E21Z Health Care | $353.6M | 16 |
| COLLEGIATE HOUSING FOUNDATION | Fairhope | L400 Housing & Shelter | $336.2M | 10 |
| Samford University | Birmingham | B430 Education | $299.7M | 12 |
| THE HEALTH CARE AUTHORITY OF THE CITY OF ANNISTON | Anniston | E220 Health Care | $281M | 11 |
| CORPORATE AMERICA CREDIT UNION | Irondale | — | $230.3M | 13 |
| Tuskegee University | Tuskegee Institute | B430 Education | $224.8M | 22 |
| BALDWIN COUNTY ELECTRIC MEMBERSHIP CORPORATION | Summerdale | — | $223.3M | 16 |
| Joe Wheeler Electric Membership Corporation | Trinity | — | $219.2M | 19 |
| CULLMAN REGIONAL MEDICAL CENTER INC | Cullman | E220 Health Care | $191.1M | 6 |
| St Vincent's Birmingham | Birmingham | E220 Health Care | $182.6M | 0 |
| Cullman Electric Cooperative | Cullman | — | $167.9M | 17 |
| NOLAND HEALTH SERVICES INC | Birmingham | P75 Human Services | $148.5M | 23 |
| AUBURN UNIVERSITY FOUNDATION | Auburn | B110 Education | $139.2M | 16 |
| CENTRAL ALABAMA ELECTRIC COOPERATIVE | Prattville | — | $134.2M | 21 |
| SOUTHERN POVERTY LAW CENTER INC | Montgomery | I83Z Crime & Legal-Related | $129.1M | 18 |
| UNITED WAY OF CENTRAL ALABAMA INC | Birmingham | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $128.2M | 8 |
| MAX CREDIT UNION | Montgomery | — | $123.8M | 14 |
| APCO EMPLOYEES CREDIT UNION | Birmingham | — | $119.3M | 20 |
| CIVIL AIR PATROL INC | Maxwell Afb | M230 Public Safety, Disaster Preparedness & Relief | $108.6M | 9 |
| ALABAMA CREDIT UNION | Tuscaloosa | — | $106.3M | 15 |
| HAPLOTACE FOUNDATION | Birmingham | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $97.9M | 5 |
| GUARDIAN CREDIT UNION | Prattville | — | $93.7M | 12 |
| CORPORATION FOR THE PROMOTION OF RIFLE PRACTICE AND FIREARMS SAFETY INC | Anniston | N70 Recreation & Sports | $92.7M | 15 |
| AVADIAN CREDIT UNION | Birmingham | — | $90M | 14 |
| SAND MOUNTAIN ELECTRIC COOPERATIVE | Rainsville | — | $89.6M | 20 |
| THE COMMUNITY FOUNDATION OF GREATER BIRMINGHAM | Birmingham | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $89.4M | 5 |
| DIXIE ELECTRIC COOPERATIVE | Montgomery | — | $88.3M | 16 |
| INFIRMARY HEALTH SYSTEM INC | Mobile | E210 Health Care | $86.2M | 22 |
| St Vincent's East | Birmingham | E220 Health Care | $84.3M | 0 |
| TALLAPOOSA RIVER ELECTRIC COOP INC | Lafayette | — | $84.2M | 10 |
| FAULKNER UNIVERSITY | Montgomery | B420 Education | $83.6M | 10 |
| CREDIT UNIONS IN THE STATE OF ALABAMA FAMILY SAVINGS CREDIT UNION | Rainbow City | — | $83.4M | 12 |
| CREDIT UNIONS IN THE STATE OF AL LISTERHILL CREDIT UNION | Sheffield | — | $82.1M | 15 |
| FIVE STAR CREDIT UNION | Dothan | — | $80.1M | 15 |
| EQUAL JUSTICE INITIATIVE | Montgomery | R63 Civil Rights, Social Action & Advocacy | $79.6M | 8 |
| ALABAMA ONE CREDIT UNION | Tuscaloosa | — | $79.2M | 12 |
| ETERNAL WORD TELEVISION NETWORK INC | Irondale | X820 Religion-Related | $79M | 8 |
| Alabama Children's Hospital Foundation | Birmingham | E110 Health Care | $76.1M | 3 |
| Feeding the Gulf Coast | Theodore | K31 Food, Agriculture & Nutrition | $74.4M | 1 |
| OAKWOOD UNIVERSITY | Huntsville | — | $71.9M | 10 |
| COVINGTON ELECTRIC COOPERATIVE INC | Andalusia | — | $71.2M | 12 |
| RUSSELL HOSPITAL CORPORATION | Alexander City | E220 Health Care | $71.1M | 12 |
| Tennessee Valley Authority Cherokee Electric Cooperative | Centre | — | $65.1M | 9 |
| UAB MEDICINE ENTERPRISE | Birmingham | E99 Health Care | $64.4M | 14 |
