| Winston Family Foundation | Jacksonville | — | $28.3M | 2 |
| Association of Poinciana Villages | Kissimmee | — | $28.3M | 0 |
| FLORIDA VETERINARY MEDICINE FACULTY | Gainesville | D034 Animal-Related | $28.2M | 13 |
| THE EUNICE JOYCE GARDINER CHARITABLE | Palm Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $28.2M | 2 |
| SOUTHWEST FLORIDA COMMUNITY FOUNDATION | Fort Myers | T31Z Philanthropy, Voluntarism & Grantmaking Foundations | $28.1M | 5 |
| PINES OF SARASOTA HEALTHCARE LLC | Sarasota | P20 Human Services | $28.1M | 10 |
| YOUTH AND FAMILY ALTERNATIVES INC | New Port Richey | P400 Human Services | $28.1M | 2 |
| INTL ASSN AMUSEMENT PARKS & ATTRACTIONS | Orlando | — | $27.7M | 9 |
| AVANT GARDE ACADEMY FOUNDATION | Hollywood | B11 Education | $27.7M | 3 |
| Closehaul Capital Charitable Inc | Miami | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $27.6M | 0 |
| THE HEBREW DAY SCHOOL OF BROWARD COUNTY | Davie | — | $27.5M | 7 |
| THE NATIONAL CENTER FOR | Alachua | J220 Employment | $27.5M | 9 |
| STATE CHARTERED CREDIT UNIONS IN FLORIDA | Tampa | — | $27.4M | 13 |
| ST JOHN'S REHABILITATION HOSPITAL | Lauderdale Lakes | E50 Health Care | $27.3M | 9 |
| Discover Palm Beach Inc | West Palm Beach | — | $27.3M | 5 |
| RIVER GARDEN HEBREW HOME FOR THE AGED INC | Jacksonville | E910 Health Care | $27M | 9 |
| The United Way of Lee County Inc | Fort Myers | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $27M | 4 |
| RUTH & NORMAN RALES JEWISH FAMILY | Boca Raton | F60 Mental Health & Crisis Intervention | $26.8M | 5 |
| SOUTH COUNTY MENTAL HEALTH CENTER | Delray Beach | F320 Mental Health & Crisis Intervention | $26.7M | 6 |
| INNOVATIONS FINANCIAL CREDIT UNION | Panama City | W61 Public & Societal Benefit | $26.7M | 8 |
| THE CHARLES LAFITTE FLORIDA FOUNDATION | Jupiter | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $26.6M | 0 |
| PLANNED PARENTHOOD OF SOUTH FLORIDA | West Palm Beach | E42 Health Care | $26.6M | 9 |
| MAX PLANCK FLORIDA CORPORATION | Jupiter | H05 Medical Research | $26.4M | 11 |
| PLANNED PARENTHOOD OF SOUTHWEST | Sarasota | E420 Health Care | $26.3M | 15 |
| CANTERBURY SCHOOL INC | Fort Myers | B200 Education | $26.3M | 6 |
| ALFRED B MACLAY JR DAY SCHOOL | Tallahassee | — | $26.3M | 3 |
| OXBRIDGE ACADEMY FOUNDATION INC | West Palm Beach | B11 Education | $26.2M | 10 |
| FLORIDA BANKERS HEALTH CONSORTIUM INC | Winter Park | Y34 Mutual & Membership Benefit | $26.2M | 0 |
| St Joseph's Health Care Center Inc | Tampa | E210 Health Care | $26.1M | 9 |
| LAWFARE DEFENSE FUND INC | Venice | — | $26.1M | 0 |
| UNITED WAY OF BROWARD COUNTY INC | Fort Lauderdale | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $26M | 9 |
| MIA RAYS SQUADRON INC | Marco Island | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $26M | 0 |
| JEWISH FEDERATION OF SOUTH PALM | Boca Raton | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $26M | 7 |
| TAMPA PREPARATORY SCHOOL INC | Tampa | B250 Education | $25.9M | 5 |
| CHAPMAN PARTNERSHIP INC | Miami | P850 Human Services | $25.8M | 9 |
| PALM BEACH DAY ACADEMY INC | Palm Beach | B24Z Education | $25.8M | 4 |
| ST MARK VILLAGE INC | Palm Harbor | P750 Human Services | $25.7M | 7 |
| GivenGain Foundation USA | Fort Lauderdale | T50 Philanthropy, Voluntarism & Grantmaking Foundations | $25.7M | 1 |
| THE CONSORTIUM OF FLORIDA EDUCATION | Gainesville | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $25.6M | 1 |
| MIAMI ASSOCIATION OF REALTORS INC | Miami | — | $25.6M | 9 |
| THE PENG ZHAO AND CHERRY CHEN FOUNDATION | Miami | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $25.5M | 0 |
| PROJECT ACCESS FOUNDATION INC | Miami | B90 Education | $25.5M | 8 |
| THE RENWICK FOUNDATION - USA | Longboat Key | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $25.5M | 0 |
| SUNCOAST MANOR RETIREMENT COMMUNITY | Orlando | — | $25.4M | 8 |
| FLORIDA STATE UNIVERSITY CREDIT UNION | Tallahassee | — | $25.4M | 9 |
| Lightshare Behavioral Wellness & Recovery Inc | Sarasota | F220 Mental Health & Crisis Intervention | $25.4M | 5 |
| PLACE OF HOPE FOUNDATION INC | Palm Beach Gardens | P12 Human Services | $25.2M | 1 |
| COVENANT LIVING OF FLORIDA INC | Plantation | P750 Human Services | $25.1M | 18 |
| CARRFOUR SUPPORTIVE HOUSING INC | Miami | L99Z Housing & Shelter | $25M | 5 |
| BROWARD COLLEGE FOUNDATION INC | Fort Lauderdale | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $25M | 4 |
