| ORLANDO CREDIT UNION | Orlando | — | $23.1M | 7 |
| JONATHAN'S LANDING GOLF CLUB INC | Jupiter | N50 Recreation & Sports | $23.1M | 7 |
| Episcopal Day Private School of Manatee | Bradenton | B200 Education | $23M | 5 |
| AVE MARIA SCHOOL OF LAW | Naples | B50 Education | $22.9M | 7 |
| FLORIDA STATE UNIVERSITY INTERNATIONAL | Tallahassee | — | $22.9M | 13 |
| CONCORDIA OF FLORIDA INC | Tampa | L22 Housing & Shelter | $22.9M | 14 |
| PHILLIP AND PATRICIA FROST MUSEUM OF | Miami | A570 Arts, Culture & Humanities | $22.8M | 12 |
| SHADOW WOOD COUNTRY CLUB INC | Bonita Springs | N50 Recreation & Sports | $22.7M | 8 |
| OCEAN REEF COMMUNITY FOUNDATION INC | Key Largo | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $22.6M | 2 |
| The Arc of the St Johns Inc | Saint Augustine | P820 Human Services | $22.5M | 1 |
| Early Learning Coalition of Florida's Gateway Inc | Lake City | B012 Education | $22.5M | 2 |
| UNITED STATES SPECIALTY SPORTS | Melbourne | — | $22.5M | 17 |
| EPWORTH VILLAGE WEST INC | Hialeah | L220 Housing & Shelter | $22.5M | 1 |
| Cove Behavioral Health Inc | Tampa | F22O Mental Health & Crisis Intervention | $22.3M | 8 |
| THE ZOOLOGICAL SOCIETY OF THE PALM | West Palm Beach | D500 Animal-Related | $22.3M | 5 |
| EPISCOPAL-CATHOLIC APARTMENTS INC | Winter Haven | — | $22.1M | 0 |
| RENAISSANCE ARTS AND EDUCATION INC | Palmetto | B24Z Education | $22.1M | 3 |
| UNITED CEREBRAL PALSY ASSOCIATION | Hialeah | G20 Diseases, Disorders & Medical Disciplines | $22M | 3 |
| THE CLUB AT MEDITERRA INC | Naples | N6A Recreation & Sports | $22M | 5 |
| Michael-Ann Russell Jewish Community | North Miami Beach | P200 Human Services | $21.9M | 7 |
| VERO BEACH MUSEUM OF ART INC | Vero Beach | A25Z Arts, Culture & Humanities | $21.8M | 3 |
| CENTRAL MANOR APARTMENTS | Ormond Beach | — | $21.8M | 0 |
| HEART OF FLORIDA UNITED WAY INC | Orlando | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $21.8M | 7 |
| The Master's Academy | Oviedo | — | $21.8M | 7 |
| THE FOUNDATION FOR OSCEOLA | Kissimmee | B29 Education | $21.8M | 1 |
| THE ICAHN MEDICAL RESEARCH FOUNDATION | Sunny Isles Beach | E05 Health Care | $21.7M | 1 |
| PINELLAS COMMUNITY FOUNDATION | Clearwater | T31Z Philanthropy, Voluntarism & Grantmaking Foundations | $21.7M | 1 |
| YOUNG MENS CHRISTIAN ASSOCIATION OF | St Petersburg | P270 Human Services | $21.6M | 3 |
| Step Up Suncoast Inc | Sarasota | S30Z Community Improvement & Capacity Building | $21.6M | 7 |
| CHARLOTTE BEHAVIORAL HEALTH CARE | Punta Gorda | F32Z Mental Health & Crisis Intervention | $21.6M | 5 |
| GLOBAL LEADERSHIP ACADEMY INC | Jacksonville | B29 Education | $21.5M | 4 |
| UNIVERSITY OF CENTRAL FLORIDA RESEARCH | Orlando | T22Z Philanthropy, Voluntarism & Grantmaking Foundations | $21.5M | 6 |
| DIRECTIONS FOR MENTAL HEALTH INC | Clearwater | — | $21.5M | 6 |
| WONDERFUL LIFE FOUNDATION | West Palm Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $21.5M | 0 |
| HOSPICE OF HEALTH FIRST INC | Rockledge | P740 Human Services | $21.4M | 13 |
| HABITAT FOR HUMANITY OF BROWARD INC | Fort Lauderdale | — | $21.4M | 4 |
| ARISON ARTS FOUNDATION | Coral Gables | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $21.3M | 2 |
| SARASOTA-MANATEE JEWISH HOUSING COUNCIL | Sarasota | P750 Human Services | $21.3M | 6 |
| UNITED JEWISH COMMUNITY OF BROWARD | Davie | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $21.3M | 8 |
| SAINT EDWARD'S SCHOOL INC | Vero Beach | B200 Education | $21.3M | 6 |
| DONNA KLEIN JEWISH ACADEMY INC | Boca Raton | B20 Education | $21.3M | 3 |
| Harvesters Credit Union | Cantonment | — | $21.2M | 15 |
| LE JARDIN COMMUNITY CENTER INC | Homestead | B210 Education | $21.2M | 2 |
| AIDS SERVICE ASSOCIATION OF PINELLAS INC | Lakewood Ranch | — | $21.1M | 5 |
| PANAMA CITY BEACH CONVENTION | Panama City Beach | S41 Community Improvement & Capacity Building | $21.1M | 1 |
| FEEDING FLORIDA INC | Tallahassee | — | $21.1M | 1 |
| NATIONAL GIFT ANNUITY FOUNDATION | Jacksonville | T21Z Philanthropy, Voluntarism & Grantmaking Foundations | $21M | 0 |
| TRI-COUNTY HUMAN SERVICES INC | Lakeland | F210 Mental Health & Crisis Intervention | $20.9M | 8 |
| DOCTORS' MEMORIAL HOSPITAL INC | Perry | E22 Health Care | $20.9M | 7 |
| THE COMMUNITY FOUNDATION - | Stuart | P20 Human Services | $20.9M | 1 |
