| SALVADOR DALI MUSEUM INC | St Petersburg | A510 Arts, Culture & Humanities | $19.2M | 4 |
| TAMPA BAY WORKFORCE ALLIANCE INC | Tampa | W20 Public & Societal Benefit | $19.1M | 9 |
| SUNCOAST COMMUNITIES BLOOD BANK INC | Bradenton | E61Z Health Care | $19.1M | 3 |
| BRAVO FAMILY CHARITABLE FOUNDATION | Miami | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $19.1M | 0 |
| East Orlando Health & Rehab | Altamonte Springs | — | $19.1M | 11 |
| EVANGELICAL CHRISTIAN SCHOOL INC | Fort Myers | B24Z Education | $19M | 1 |
| VIERA CHARTER SCHOOLS INC | Viera | B20 Education | $19M | 1 |
| BOYS & GIRLS CLUBS OF CENTRAL FLORIDA INC | Orlando | O230 Youth Development | $19M | 5 |
| UFCW & EMPLOYERS KANSAS & MISSOURI | Pembroke Pines | — | $19M | 2 |
| FLORIDA STATE UNIVERSITY RESEARCH | Tallahassee | B110 Education | $19M | 10 |
| TIMUQUANA COUNTRY CLUB | Jacksonville | — | $18.9M | 7 |
| JUPITER CHRISTIAN SCHOOL INC | Jupiter | — | $18.9M | 2 |
| CENTER FOR FAMILY & CHILD ENRICHMENT INC | Miami Gardens | F30Z Mental Health & Crisis Intervention | $18.9M | 6 |
| JEWISH COMMUNITY CENTERS OF SOUTH | Davie | P280 Human Services | $18.7M | 5 |
| ELMLAND COMMUNITY FOUNDATION INC | Delray Beach | L21 Housing & Shelter | $18.7M | 0 |
| BOYS AND GIRLS CLUBS OF NORTHEAST | Jacksonville | O23Z Youth Development | $18.7M | 6 |
| BELLEVIEW BILTMORE COUNTRY CLUB CORP | Belleair | — | $18.7M | 6 |
| Free & Accepted Masons of Florida Most | Jacksonville | Y40Z Mutual & Membership Benefit | $18.7M | 2 |
| THEODORE R AND VIVIAN M | West Palm Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $18.7M | 10 |
| EG Watkins Family Foundation | Palm Beach Gardens | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $18.7M | 0 |
| AIR FORCE ENLISTED VILLAGE INC | Shalimar | L50 Housing & Shelter | $18.7M | 3 |
| THE SOCIETY OF THE FOUR ARTS INC | Palm Beach | A200 Arts, Culture & Humanities | $18.5M | 8 |
| LINCOLN-MARTI CHARTER SCHOOLS INC | Miami | B29 Education | $18.5M | 6 |
| FLORIDA STUDIO THEATRE INC | Sarasota | A650 Arts, Culture & Humanities | $18.5M | 5 |
| THE STILES-NICHOLSON FOUNDATION | Jupiter | — | $18.5M | 0 |
| INTERNATIONAL STUDIES CHARTER | Miami | B29 Education | $18.5M | 2 |
| WESTMINSTER SERVICES INC | Orlando | L02 Housing & Shelter | $18.4M | 9 |
| THOMAS W SMITH FOUNDATION INC | Boca Raton | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $18.4M | 4 |
| Orangewood Christian School Inc | Maitland | B20 Education | $18.3M | 5 |
| The Colen Family Charitable Trust | Ocala | T23 Philanthropy, Voluntarism & Grantmaking Foundations | $18.3M | 1 |
| Restoration Bridge International Inc | Lake Worth | P20 Human Services | $18.2M | 1 |
| J CHRISTOPHER AND | West Palm Beach | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $18.2M | 0 |
| PORT OF MIAMI CRANE MANAGEMENT INC | Miami | W99 Public & Societal Benefit | $18.2M | 6 |
| CRYSTAL LAKE SUPPORTIVE ENVIRONMENTS | Orlando | E50Z Health Care | $18.2M | 3 |
| THE TWINEAGLES CLUB INC | Naples | N6A Recreation & Sports | $18.2M | 8 |
| FIRST COAST WORKFORCE DEVELOPMENT INC | Fleming Island | J20 Employment | $18.1M | 1 |
| PACE WATER SYSTEM INC | Pace | — | $18.1M | 2 |
| AGAPE NETWORK INC | Miami | I43Z Crime & Legal-Related | $18.1M | 12 |
| BIG CAT RESCUE CORP | Odessa | P30 Human Services | $18.1M | 3 |
| Jessie Ball duPont Religious Charitable | Jacksonville | — | $18.1M | 9 |
| THE EVERGLADES FOUNDATION INC | Palmetto Bay | C012 Environment | $18M | 11 |
| EASTER SEALS SOUTH FLORIDA INC | Miami | E50Z Health Care | $18M | 5 |
| THE FIRST NATION GROUP FOUNDATION INC | Niceville | W30 Public & Societal Benefit | $17.9M | 0 |
| ADMIRAL FARRAGUT ACADEMY INC | St Petersburg | B250 Education | $17.9M | 6 |
| TWELVE STARS FOUNDATION | Palm Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $17.9M | 0 |
| CORNERSTONE CHARTER ACADEMY INC | Miami | B29 Education | $17.9M | 1 |
| North Florida Medical Centers Inc | Tallahassee | E30C Health Care | $17.9M | 8 |
| COPPERHEAD CHARITIES INC | Palm Harbor | T990 Philanthropy, Voluntarism & Grantmaking Foundations | $17.8M | 4 |
| HARBOUR RIDGE YACHT & COUNTRY CLUB | Palm City | — | $17.8M | 8 |
| Health Care Center For The Homeless Inc | Orlando | F99Z Mental Health & Crisis Intervention | $17.8M | 11 |
