| BATTELLE MEMORIAL INSTITUTE | Columbus | OH | $13.3B | 32 |
| ADVANCED TECHNOLOGY INTERNATIONAL | Summerville | SC | $4.8B | 25 |
| THE MITRE CORPORATION | McLean | VA | $2.4B | 48 |
| Research Triangle Institute | Research Triangle Park | NC | $1.4B | 34 |
| The Aerospace Corporation | Chantilly | VA | $1.4B | 42 |
| NAVIGATION CHARITABLE FUND | Berkeley | CA | $1.3B | 1 |
| Southwest Research Institute | San Antonio | TX | $926.4M | 36 |
| CHARLES STARK DRAPER LABORATORY INC | Cambridge | MA | $871.1M | 24 |
| AMERICAN CHEMICAL SOCIETY | Washington | DC | $819.1M | 12 |
| ALLIANCE FOR SUSTAINABLE ENERGY LLC | Golden | CO | $786.3M | 10 |
| Fermi Research Alliance LLC | Batavia | IL | $715.5M | 31 |
| NOBLIS INC | Reston | VA | $621.3M | 27 |
| BLUEFORGE ALLIANCE | Bryan | TX | $480.9M | 12 |
| ASSOCIATION OF UNIVERSITIES FOR RESEARCH | Washington | DC | $450.4M | 23 |
| SRI INTERNATIONAL | Menlo Park | CA | $437.9M | 28 |
| CLIMATEWORKS FOUNDATION | San Francisco | CA | $350.6M | 15 |
| INSTITUTE FOR DEFENSE ANALYSES | Alexandria | VA | $350.3M | 25 |
| WOODS HOLE OCEANOGRAPHIC INSTITUTION | Woods Hole | MA | $345.9M | 11 |
| NATIONAL ACADEMY OF SCIENCES | Washington | DC | $324.6M | 17 |
| UNIVERSITY CORPORATION FOR ATMOSPHERIC | Boulder | CO | $311M | 21 |
| COLD SPRING HARBOR LABORATORY | Cold Spring Harbor | NY | $258M | 12 |
| SOUTHEASTERN UNIVERSITIES RESEARCH | Washington | DC | $242.1M | 19 |
| MEDICAL TECHNOLOGY ENTERPRISE CONSORTIUM | Summerville | SC | $234.5M | 3 |
| SETI INSTITUTE | Mountain View | CA | $209.9M | 9 |
| AMERICAN TYPE CULTURE COLLECTION | Manassas | VA | $198M | 23 |
| GMTO CORPORATION | Pasadena | CA | $194.7M | 11 |
| PREPASS SAFETY ALLIANCE | Phoenix | AZ | $193.2M | 7 |
| University of Kansas Center for Research Inc | Lawrence | KS | $191.7M | 29 |
| The Hawaii Pacific Foundation Inc | Honolulu | HI | $187.9M | 3 |
| NATIONAL ADVANCED MOBILITY CONSORTIUM | Ann Arbor | MI | $187.6M | 6 |
| Internet Corporation for Assigned Names and Numbers | Los Angeles | CA | $184.8M | 37 |
| LIGHTCONE FOUNDATION | Sparks | NV | $163.3M | 0 |
| The Salk Institute for Biological Studies | La Jolla | CA | $161.2M | 12 |
| THE AMERICAN SOCIETY OF MECHANICAL | New York | NY | $160.5M | 10 |
| High Q Foundation Inc | Roseland | NJ | $152.2M | 0 |
| ASSOCIATED UNIVERSITIES INC | Vienna | VA | $143.5M | 13 |
| ANALYTIC SERVICES INC | Arlington | VA | $138.6M | 24 |
| CARNEGIE INSTITUTION OF WASHINGTON | Washington | DC | $129.1M | 16 |
| AMERICAN ASSOCIATION FOR THE ADVANCEMENT | Washington | DC | $123.2M | 15 |
| GEORGIA SOUTHERN UNIVERSITY | Statesboro | GA | $121.7M | 5 |
| American Physical Society | College Park | MD | $118.8M | 13 |
| Institute of Gas Technology | Des Plaines | IL | $118M | 17 |
| MRIGlobal | Kansas City | MO | $118M | 35 |
| DEFENSEWERX INC | Niceville | FL | $112.5M | 1 |
| NATIONAL CENTER FOR DEFENSE MANUFACTURING AND MACHINING | Johnstown | PA | $112.2M | 21 |
| THE ALLEN INSTITUTE FOR ARTIFICIAL | Seattle | WA | $110.9M | 7 |
| AMERICAN SOCIETY FOR TESTING | West Conshohocken | PA | $106.9M | 27 |
| Public Interest Registry | Reston | VA | $105.1M | 21 |
| CONCURRENT TECHNOLOGIES CORPORATION | Johnstown | PA | $103M | 21 |
| American Institute of Physics Inc | College Park | MD | $100M | 19 |
