| JOHN T VUCUREVICH FOUNDATION | Rapid City | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $8.6M | 9 |
| RAPID CITY Y M C A | Rapid City | P270 Human Services | $8.6M | 3 |
| CAM WAL ELECTRIC COOPERATIVE INC | Selby | — | $8.5M | 14 |
| DAKOTA COUNSELING INSTITUTE INC | Mitchell | F30Z Mental Health & Crisis Intervention | $8.2M | 1 |
| LONEMAN SCHOOL CORPORATION | Oglala | B24I Education | $8.2M | 4 |
| MINNEHAHA COMMUNITY WATER CORP | Dell Rapids | — | $8M | 8 |
| DAVID H BILLION FOUNDATION | Sioux Falls | I12 Crime & Legal-Related | $7.9M | 0 |
| MARTY INDIAN SCHOOL BOARD INC | Marty | B20Z Education | $7.8M | 0 |
| MOREAU GRAND ELECTRIC COOPERATIVE | Timber Lake | — | $7.7M | 14 |
| South Lincoln Rural Water System Inc | Beresford | — | $7.7M | 8 |
| Sesdac Inc | Vermillion | F330 Mental Health & Crisis Intervention | $7.6M | 0 |
| CAPITAL AREA COUNSELING SERVICEINC | Pierre | F32Z Mental Health & Crisis Intervention | $7.6M | 1 |
| NORTHERN HILLS TRAINING CENTER INC | Spearfish | J30Z Employment | $7.5M | 1 |
| BOYS & GIRLS CLUB OF THE NORTHERN PLAINS INC | Brookings | O23 Youth Development | $7.4M | 5 |
| HARVEY W PETERS RESEARCH FOUNDATION C /O FIRST PREMIER BANK TRUST DEPT | Sioux Falls | H99 Medical Research | $7.3M | 0 |
| United Retirement Center | Brookings | P700 Human Services | $7.2M | 1 |
| SUNSET MANOR INC | Irene | E91Z Health Care | $7.2M | 2 |
| ELEVATE RAPID CITY | Rapid City | — | $7.2M | 1 |
| TIESZEN MEMORIAL HOME | Marion | Z99Z Unknown | $7.2M | 9 |
| WAITT INSTITUTE | North Sioux City | A05 Arts, Culture & Humanities | $7.2M | 1 |
| SOUTH DAKOTA LIONS EYE AND TISSUE BANK INC | Sioux Falls | E99Z Health Care | $7M | 1 |
| INDIAN BOARD OF EDUCATION FOR THE PIERRE INDIAN SCHOOL | Pierre | P620 Human Services | $7M | 15 |
| SOUTH DAKOTA URBAN INDIAN HEALTH INC | Sioux Falls | — | $7M | 2 |
| Enemy Swim Day School | Waubay | — | $6.9M | 7 |
| LANDMANN-JUNGMAN MEMORIAL HOSPITAL CORPORATION | Scotland | E22 Health Care | $6.9M | 5 |
| YELLOW JACKET FOUNDATION | Spearfish | — | $6.9M | 1 |
| LAKE AREA IMPROVEMENT CORPORATION | Madison | S30Z Community Improvement & Capacity Building | $6.9M | 1 |
| THE VILLAGE OF HARMONY HILL INC | Watertown | L22 Housing & Shelter | $6.7M | 0 |
| BETHEL LUTHERAN HOME | Madison | P750 Human Services | $6.7M | 1 |
| Northeastern Mental Health Center | Aberdeen | F32Z Mental Health & Crisis Intervention | $6.6M | 2 |
| The Carroll Institute | Sioux Falls | F34Z Mental Health & Crisis Intervention | $6.5M | 2 |
| Aberdeen Family YMCA | Aberdeen | — | $6.4M | 2 |
| WEST RIVER LYMAN JONES RURAL WATER SYSTEMS | Murdo | — | $6.3M | 12 |
| SOUTH DAKOTA LIFE AND HEALTH INSURANCE GUARANTY ASSOCIATION | Sioux Falls | — | $6.1M | 0 |
| CORNERSTONE RESCUE MISSION | Rapid City | — | $6.1M | 1 |
| THE EVANGELICAL LUTHERAN GOOD SAMARITAN FOUNDATION | Sioux Falls | X20 Religion-Related | $6M | 5 |
| COMMUNITY COUNSELING SERVICES | Huron | — | $6M | 4 |
| HELPLINE CENTER INC | Sioux Falls | — | $5.9M | 1 |
| THE LUKE SOCIETY INC | Sioux Falls | — | $5.9M | 3 |
| Brookings Deuel Rural Water System | Toronto | — | $5.8M | 8 |
| EUREKA COMMUNITY BENEVOLENT HOSPITAL ASSOCIATION | Eureka | E22 Health Care | $5.8M | 4 |
| VALIANT LIVING INCORPORATED | Madison | J330 Employment | $5.7M | 2 |
| ADITI FOUNDATION C/O SOUTH DAKOTA TRUST COMPANY LLC TTE | Sioux Falls | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $5.6M | 4 |
| BETHESDA OF BERESFORD | Beresford | — | $5.6M | 1 |
| Grant-Roberts Rural Water System Inc | Milbank | — | $5.5M | 8 |
| BLACK HILLS EDUCATIONAL INSTITUTE INC | Rapid City | B64Z Education | $5.5M | 0 |
| BETHESDA HOME INC | Webster | — | $5.4M | 0 |
| BRANCHES FOUNDATION | Sioux Falls | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $5.4M | 0 |
| ESTHER AND HAROLD MERTZ FOUNDATION C/O DIANNE R STADTFELD SDTC LLC | Sioux Falls | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $5.4M | 2 |
| DAKOTA BIOWORX | Brookings | S30 Community Improvement & Capacity Building | $5.4M | 1 |
