| TIOSPA ZINA TRIBAL SCHOOL | Agency Village | B20Z Education | $13.4M | 0 |
| BEHAVIOR MANAGEMENT SYSTEMS INC | Rapid City | — | $13.2M | 5 |
| THE ROBERT AND RITA ELMEN FOUNDATION | Sioux Falls | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $13.1M | 4 |
| DOUGLAS COUNTY MEMORIAL HOSPITAL | Armour | E22Z Health Care | $12.8M | 7 |
| Avera Gettysburg | Gettysburg | E22Z Health Care | $12.8M | 12 |
| SIOUX FALLS ZOO & AQUARIUM | Sioux Falls | D500 Animal-Related | $12.8M | 0 |
| BENNETT COUNTY HOSPITAL AND NURSING HOME | Martin | E20 Health Care | $12.8M | 6 |
| NDN COLLECTIVE INC | Rapid City | S50 Community Improvement & Capacity Building | $12.6M | 20 |
| ABBOTT HOUSE INC | Mitchell | P730 Human Services | $12.6M | 2 |
| WHETSTONE VALLEY ELECTRIC COOPERATIVE | Milbank | — | $12.6M | 14 |
| Kingbrook Rural Water System Inc | Arlington | — | $12.5M | 8 |
| RUSHMORE ELECTRIC POWER COOP | Rapid City | — | $12.4M | 14 |
| SANFORD SCHOOL OF MEDICINE OF THE | Sioux Falls | B54Z Education | $12.4M | 3 |
| BON HOMME YANKTON ELECTRIC ASSOCIATION | Tabor | — | $12.4M | 12 |
| ADITI FOUNDATION | Sioux Falls | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $12.1M | 4 |
| FAULKTON AREA MEDICAL CENTER | Faulkton | E22 Health Care | $12M | 8 |
| THE LAKOTA FUND INCORPORATED | Kyle | S430 Community Improvement & Capacity Building | $11.5M | 9 |
| Aspire Inc | Aberdeen | J300 Employment | $11.4M | 2 |
| THE BABINSKI FOUNDATION | Sioux Falls | D20 Animal-Related | $11.1M | 4 |
| NATIVE AMERICAN VETERANS ASSISTANCE | Pine Ridge | P20 Human Services | $11M | 2 |
| CHILDREN'S HOME FOUNDATION | Sioux Falls | P730 Human Services | $10.7M | 2 |
| ABILITY BUILDING SERVICES INC | Yankton | P820 Human Services | $10.7M | 2 |
| COMMUNITY HEALTH CENTER OF THE BLACK | Rapid City | — | $10.6M | 7 |
| CLAY-UNION ELECTRIC CORPORATION | Vermillion | — | $10.6M | 10 |
| BUTTE ELECTRIC COOPERATIVE INC | Newell | — | $10.5M | 11 |
| UNIVERSITY OF SOUTH DAKOTA RESEARCH PARK | Sioux Falls | B90 Education | $10.4M | 2 |
| MCCROSSAN BOYS RANCH | Sioux Falls | F33Z Mental Health & Crisis Intervention | $10.3M | 1 |
| FOUR BANDS COMMUNITY FUND INC | Eagle Butte | S30 Community Improvement & Capacity Building | $10.3M | 1 |
| BOYS & GIRLS CLUBS OF THE SIOUX EMPIRE | Sioux Falls | P36Z Human Services | $10.3M | 4 |
| DAKOTA STATE UNIVERSITY FOUNDATION | Madison | B431 Education | $10.2M | 2 |
| GROW SOUTH DAKOTA | Sisseton | S30 Community Improvement & Capacity Building | $10M | 3 |
| RURAL AMERICA INITIATIVES | Rapid City | S32Z Community Improvement & Capacity Building | $9.8M | 1 |
| ROSEBUD ELECTRIC COOPERATIVE INC | Gregory | — | $9.6M | 12 |
| REGENCY MANAGED PROPERTIES BENEFIT TRUST | Sioux Falls | — | $9.5M | 0 |
| EMBE | Sioux Falls | — | $9.5M | 2 |
| RAPID CITY Y M C A | Rapid City | P270 Human Services | $9.4M | 2 |
| OUR HOME INC | Huron | — | $9.4M | 2 |
| SIOUX FALLS AREA HUMANE SOCIETY | Sioux Falls | D200 Animal-Related | $9.3M | 1 |
| FIRST PEOPLES FUND | Rapid City | A23 Arts, Culture & Humanities | $9.2M | 7 |
| SEEDS OF CHANGE FOUNDATION | Sioux Falls | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $9.1M | 0 |
| BDM RURAL WATER SYSTEM INC | Britton | — | $9.1M | 0 |
| COMMUNITY MEMORIAL HOSPITAL INC | Burke | — | $9M | 5 |
| THE SUMMIT LEAGUE | Sioux Falls | — | $9M | 2 |
| SANFORD HEALTH FOUNDATION NORTH | Sioux Falls | E122 Health Care | $8.8M | 5 |
| SIOUX EMPIRE UNITED WAY INC | Sioux Falls | — | $8.8M | 3 |
| Community Coordinated Transportation Sys | Pierre | W40 Public & Societal Benefit | $8.8M | 3 |
| ADVANCE | Brookings | P8XZ Human Services | $8.7M | 1 |
| Minnehaha Country Club | Sioux Falls | — | $8.7M | 6 |
| JOHN T VUCUREVICH FOUNDATION | Rapid City | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $8.6M | 9 |
| CAM WAL ELECTRIC COOPERATIVE INC | Selby | — | $8.5M | 14 |
