| HEALTH CHOICE NETWORK OF FLORIDA INC | Miami | E32 Health Care | $56M | 14 |
| CIRCLES OF CARE INC | Melbourne | F300 Mental Health & Crisis Intervention | $55.7M | 13 |
| COMMUNITY PARTNERSHIP FOR CHILDREN INC | Daytona Beach | P20 Human Services | $55.6M | 3 |
| RIVER CITY EDUCATION SERVICES INC | Jacksonville | B29 Education | $55.5M | 1 |
| GULFSTREAM GOODWILL INDUSTRIES INC | West Palm Beach | J320 Employment | $55.5M | 9 |
| ARBITRATION FORUMS INC | Tampa | I51Z Crime & Legal-Related | $55.4M | 13 |
| ALL FAITHS FOOD BANK INC | Sarasota | K310 Food, Agriculture & Nutrition | $55.4M | 8 |
| ACADEMIR CHARTER SCHOOL INC | Miami | B29 Education | $55M | 0 |
| GULF COAST COMMUNITY FOUNDATION INC | Venice | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $54.4M | 8 |
| FLORIDA ASSOCIATION OF REHABILITATION | Tallahassee | — | $54.4M | 3 |
| GATOR BOOSTERS INC | Gainesville | — | $54.4M | 3 |
| AMIKIDS INC | Tampa | — | $54.4M | 9 |
| TAMPA METROPOLITAN AREA YOUNG MEN'S | Tampa | P270 Human Services | $54.3M | 9 |
| NORTH CENTRAL FLORIDA HOSPICE INC | Gainesville | P740 Human Services | $54.2M | 2 |
| BONITA SPRINGS UTILITIES INC | Bonita Springs | — | $53.9M | 14 |
| SPECIAL OPERATIONS WARRIOR FOUNDATION | Tampa | B82 Education | $53.9M | 6 |
| PARTNERSHIP FOR STRONG FAMILIES INC | Gainesville | E60 Health Care | $53.8M | 4 |
| MIAMI DADE COLLEGE FOUNDATION INC | Miami | B110 Education | $53.8M | 6 |
| CATHOLIC CHARITIES OF THE | Wilton Manors | P200 Human Services | $53.7M | 8 |
| Hope for Haiti Inc | Naples | Q123 International, Foreign Affairs & National Security | $53.6M | 1 |
| TAMPA BAY PERFORMING ARTS CENTER INC | Tampa | — | $53M | 10 |
| GULF COAST JEWISH FAMILY AND COMMUNITY | Clearwater | — | $52.9M | 8 |
| LITTLE HAVANA ACTIVITIES AND NUTRITION | Miami | K00Z Food, Agriculture & Nutrition | $52.7M | 5 |
| EARLY LEARNING COALITION OF NORTH | St Augustine | B21 Education | $52.7M | 1 |
| Orlando Neighborhood Improvement Foundation Inc | Orlando | — | $52.6M | 3 |
| MENTAL HEALTH CARE INC | Tampa | F31Z Mental Health & Crisis Intervention | $52.2M | 8 |
| SOCIETY OF ST VINCENT DE PAUL | St Petersburg | P20 Human Services | $52.1M | 8 |
| THE BATCHELOR FOUNDATION INC | Miami Beach | — | $52M | 4 |
| GREATER MIAMI CONVENTION & VISITORS | Miami | — | $51.8M | 9 |
| HENDERSON BEHAVIORAL HEALTH INC | Fort Lauderdale | F320 Mental Health & Crisis Intervention | $51.7M | 13 |
| JAEB CENTER FOR HEALTH RESEARCH | Tampa | Z99 Unknown | $51.6M | 9 |
| FLORIDA ATLANTIC UNIVERSITY FOUNDATION | Boca Raton | B110 Education | $51.5M | 12 |
| EMPATH-STRATUM INC | Lakewood Ranch | P74 Human Services | $51.1M | 10 |
| ADRIENNE ARSHT CENTER TRUST INC | Miami | A111 Arts, Culture & Humanities | $51.1M | 7 |
| ELISABETH C DELUCA FOUNDATION INC | Pompano Beach | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $51.1M | 0 |
| HEALTH CHOICE NETWORK INC | Miami | E60 Health Care | $51M | 12 |
| ADVANTAGE ACADEMY OF MIAMI INC | Coral Springs | B21 Education | $51M | 5 |
| UNIVERSITY OF FLORIDA | Gainesville | B110 Education | $50.9M | 20 |
| JOHN'S ISLAND CLUB INC | Indian River Shores | — | $50.7M | 9 |
| ST MATTHEWS HOUSE INC | Naples | L41 Housing & Shelter | $50.5M | 9 |
| THE BROKEN SOUND CLUB INC | Boca Raton | N50 Recreation & Sports | $50.5M | 7 |
| FEDERATION CCRC OPERATIONS CORP | Boca Raton | L22 Housing & Shelter | $50.5M | 3 |
| FLORIDA MEMORIAL UNIVERSITY INC | Miami Gardens | B420 Education | $50.4M | 11 |
| GULFSIDE HOSPICE INC | Land Olakes | — | $50.4M | 3 |
| EARLY LEARNING COALITION OF BREVARD | Rockledge | B012 Education | $50.1M | 2 |
| PLYMOUTH HARBOR INC | Sarasota | E91Z Health Care | $50.1M | 12 |
| Lincoln-Marti Community Agency Inc | Miami | B95Z Education | $50M | 3 |
| THE KING'S ACADEMY INC | West Palm Beach | B24Z Education | $50M | 9 |
| RADIANT CREDIT UNION | Gainesville | W61 Public & Societal Benefit | $49.8M | 20 |
| WE FLORIDA FINANCIAL | Pembroke Pines | — | $49.8M | 10 |
