| SEEDING SUCCESS | Memphis | O50 Youth Development | $23.1M | 6 |
| Lorraine Civil Rights Museum Foundation | Memphis | A540 Arts, Culture & Humanities | $23.1M | 8 |
| THE FRIST FOUNDATION | Nashville | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $23.1M | 3 |
| MID-CUMBERLAND COMMUNITY ACTION AGENCY | Gallatin | P20Z Human Services | $23M | 2 |
| Good Shepherd Medication Management | Memphis | Z99 Unknown | $23M | 1 |
| RICHMOND THE AMERICAN INTERNATIONAL UNIVERSITY IN LONDON INC | Nashville | — | $23M | 9 |
| TEN CREDIT UNION | Nashville | W61Z Public & Societal Benefit | $22.9M | 7 |
| DAVA HEALTH INC | Nashville | E02 Health Care | $22.8M | 0 |
| Covenant HomeCare | Knoxville | E92Z Health Care | $22.8M | 10 |
| GOODWILL INDUSTRIES - KNOXVILLE INC | Knoxville | J320 Employment | $22.7M | 4 |
| GOVERNOR'S EARLY LITERACY FOUNDATION | Nashville | B92 Education | $22.7M | 2 |
| MEDIC INC | Knoxville | E610 Health Care | $22.6M | 9 |
| Chattanooga Goodwill Industries Inc | Chattanooga | J32Z Employment | $22.6M | 5 |
| FRATERNAL ORDER OF POLICE LEGAL PLAN INC | Nashville | — | $22.5M | 7 |
| TENNESSEE STATE UNIVERSITY FOUNDATION | Nashville | B11 Education | $22.4M | 0 |
| FAMILY & CHILDREN'S SERVICE | Nashville | P400 Human Services | $22.3M | 6 |
| Thompson Oncology Group | Knoxville | E24Z Health Care | $22.2M | 12 |
| REPUBLIC SCHOOLS NASHVILLE | Nashville | B24 Education | $22.1M | 8 |
| COVENANT LIVING AT SHANNONDALE | Knoxville | P75Z Human Services | $22.1M | 0 |
| KIPP MEMPHIS | Memphis | B29 Education | $22M | 7 |
| NATIONAL COTTON COUNCIL OF AMERICA | Cordova | — | $21.9M | 14 |
| RIDGEVIEW PSYCHIATRIC HOSPITAL & CENTER INC | Oak Ridge | F310 Mental Health & Crisis Intervention | $21.9M | 9 |
| BELLE MEADE COUNTRY CLUB | Nashville | — | $21.8M | 6 |
| LOCAL GOVERNMENT CORPORATION | Columbia | W17B Public & Societal Benefit | $21.7M | 14 |
| US COMMUNITY CREDIT UNION | Nashville | — | $21.4M | 13 |
| PROGRESSIVE DIRECTIONS INC | Clarksville | P820 Human Services | $21.4M | 2 |
| APPALACHIA SERVICE PROJECT INC | Johnson City | L20Z Housing & Shelter | $21.3M | 2 |
| FORMANEK FOUNDATION | Memphis | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $21.3M | 0 |
| AUSTIN PEAY STATE UNIVERSITY FOUNDATION | Clarksville | B43I Education | $21.3M | 0 |
| NORTHWEST TENNESSEE EDC | Dresden | S200 Community Improvement & Capacity Building | $21.3M | 5 |
| POSITIVELY LIVING | Knoxville | P33 Human Services | $21.2M | 1 |
| MACON HOSPITAL INC | Lafayette | E22Z Health Care | $21.1M | 14 |
| MOUNTAIN PEOPLES HEALTH COUNCILS INC | Huntsville | — | $21.1M | 6 |
| ORPHEUM THEATRE GROUP | Memphis | S200 Community Improvement & Capacity Building | $20.9M | 6 |
| MIDWEST CHRISTIAN VILLAGES | Nashville | P81 Human Services | $20.9M | 25 |
| CHEEKWOOD BOTANICAL GARDEN AND MUSEUM OF ART | Nashville | A510 Arts, Culture & Humanities | $20.7M | 7 |
| CHRISTIAN ACADEMY OF KNOXVILLE | Knoxville | B24Z Education | $20.7M | 6 |
| THE WEBB SCHOOL | Bell Buckle | B250 Education | $20.6M | 4 |
| IRON WORKERS DISTRICT COUNCIL OF TN VALLEY & VICINITY WELFARE PLAN | Goodlettsville | — | $20.5M | 0 |
| EMORY VALLEY CENTER INC | Oak Ridge | P82Z Human Services | $20.5M | 2 |
| ASPIRE FOUNDATION | Clinton | N32 Recreation & Sports | $20.4M | 0 |
| LeMoyne-Owen College | Memphis | B42Z Education | $20.4M | 11 |
| SOUTHEAST TENNESSEE HUMAN RESOURCE AGENCY | Dunlap | P200 Human Services | $20.3M | 0 |
| MOF - PRESERVATION OF AFFORDABILITY CORP | Cordova | L20 Housing & Shelter | $20.2M | 1 |
| LAKESHORE ESTATES INC | Nashville | — | $20M | 0 |
| THE NASHVILLE CATALYST FUND | Nashville | L11 Housing & Shelter | $20M | 2 |
| BOYS & GIRLS CLUBS IN TENNESSEE | Knoxville | O192 Youth Development | $19.9M | 5 |
| TENNESSEE WILDLIFE RESOURCES FOUNDATION INC | Nashville | D113 Animal-Related | $19.8M | 2 |
| EAST TENNESSEE STATE UNIVERSITY FOUNDATION | Johnson City | B110 Education | $19.7M | 0 |
| STEM PREPARATORY ACADEMY | Nashville | B29 Education | $19.7M | 5 |
