| RARE | Arlington | C00Z Environment | $33.6M | 14 |
| RESPONSIBLE BUSINESS ALLIANCE | Alexandria | S41 Community Improvement & Capacity Building | $33.4M | 9 |
| JOHNSON HEALTH CENTER | Madison Heights | E39Z Health Care | $33.4M | 11 |
| INTERNATIONAL COPPER ASSOCIATION LTD | McLean | — | $33.4M | 7 |
| VIRGINIA EQUINE ALLIANCE | Warrenton | S40 Community Improvement & Capacity Building | $33.4M | 1 |
| FARMINGTON COUNTRY CLUB | Charlottesville | — | $33.3M | 9 |
| THE HELEN MARIE TAYLOR CHARITABLE | Richmond | A82 Arts, Culture & Humanities | $33.2M | 0 |
| LEGAL SERVICES CORP OF VIRGINIA | Richmond | I80Z Crime & Legal-Related | $32.6M | 2 |
| IMAGINE SCHOOLS INC | Vienna | B20 Education | $32.4M | 11 |
| EAST MOUNTAIN HEALTH PHYSICIANS | Winchester | E32 Health Care | $32M | 0 |
| SPORTS BETTING ALLIANCE | Fairfax | W01 Public & Societal Benefit | $31.6M | 0 |
| AIR & SPACE FORCES ASSOCIATION | Arlington | B034 Education | $31.5M | 12 |
| AMERICAN ACADEMY OF PHYSICIAN ASSOCIATES | Alexandria | — | $31.3M | 12 |
| Beth Sholom Home of Virginia | Richmond | E91Z Health Care | $31.3M | 6 |
| BATH COMMUNITY HOSPITAL | Hot Springs | E22Z Health Care | $31.2M | 8 |
| TOTAL ACTION AGAINST POVERTY IN THE | Roanoke | P200 Human Services | $31.2M | 2 |
| HORATIO ALGER ASSN OF | Alexandria | B82Z Education | $31.1M | 7 |
| Institute for Humane Studies | Arlington | — | $31.1M | 9 |
| LOUISE W EGGLESTON CENTER INC | Norfolk | P800 Human Services | $30.9M | 3 |
| ARMED SERVICES YMCA OF THE USA | Woodbridge | — | $30.8M | 19 |
| CHILD CARE AWARE OF AMERICA | Arlington | P032 Human Services | $30.7M | 11 |
| VIRGINIA LUTHERAN HOMES INC | Roanoke | — | $30.6M | 8 |
| FRIENDSHIP HEALTH AND REHAB CENTER INC | Roanoke | E910 Health Care | $30.6M | 10 |
| LOGISTICS MANAGEMENT INSTITUTE | McLean | U99 Science & Technology | $30.3M | 18 |
| Bridgewater Home Inc | Bridgewater | P75Z Human Services | $30.3M | 8 |
| BLUE INVESTMENTS FOR NATURE INC | Arlington | C30 Environment | $30.3M | 6 |
| AMERICAN CENTER FOR LAW AND JUSTICE INC | Virginia Beach | R600 Civil Rights, Social Action & Advocacy | $30.2M | 11 |
| NAVY-MARINE CORPS RELIEF SOCIETY | Arlington | W300 Public & Societal Benefit | $30.2M | 11 |
| ST MARY'S HOME FOR DISABLED CHILDREN | Norfolk | — | $30.1M | 7 |
| LEON RACHEL CORP | Springfield | — | $30M | 0 |
| CHRISTIAN RELIEF SERVICES | Alexandria | L210 Housing & Shelter | $29.9M | 5 |
| THE AMERICAN PUBLIC POWER ASSOCIATION | Arlington | — | $29.7M | 15 |
| SENIOR LIVING CHOICES INC | Midlothian | P75 Human Services | $29.7M | 4 |
| THE BARRY ROBINSON CENTER | Norfolk | P20 Human Services | $29.7M | 6 |
| LONGWOOD UNIVERSITY | Farmville | B90 Education | $29.6M | 2 |
| AMERICAN REGISTRY FOR INTERNET NUMBERS | Centreville | — | $29.4M | 13 |
| GROUP SKILLSUSA INC | Leesburg | J200 Employment | $29.4M | 0 |
| CHARLOTTESVILLE AREA COMMUNITY | Charlottesville | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $29.2M | 5 |
| HRSA-ILA FRINGE BENEFIT ESCROW FUND | Norfolk | — | $29.2M | 1 |
| AMERICAN SOCIETY OF PENSION | Arlington | — | $29.1M | 9 |
| EO COMPANIES | Abingdon | T70Z Philanthropy, Voluntarism & Grantmaking Foundations | $28.9M | 2 |
| NEWPORT NEWS BAPTIST RETIREMENT | Glen Allen | P750 Human Services | $28.8M | 9 |
| THE ROSSER FOUNDATION A PURE | Newport News | T22Z Philanthropy, Voluntarism & Grantmaking Foundations | $28.8M | 6 |
| Atlas Economic Research Foundation | Arlington | V22Z Social Science | $28.8M | 11 |
| BUCHANAN GENERAL HOSPITAL | Grundy | E220 Health Care | $28.8M | 7 |
| DANVILLE-PITTSYLVANIA COMMUNITY SERVICES | Danville | F80Z Mental Health & Crisis Intervention | $28.7M | 7 |
| COMMUNITY ELECTRIC COOPERATIVE | Windsor | — | $28.7M | 17 |
| SENTARA LIFE CARE CORPORATION | Virginia Beach | P750 Human Services | $28.4M | 15 |
| BREAD AND WATER FOR AFRICA INC | Alexandria | Q330 International, Foreign Affairs & National Security | $28.2M | 5 |
| THE MEDICAL DEVICE INNOVATION CONSORTIUM | Arlington | H99 Medical Research | $28.2M | 14 |
