| THE UNIVERSITY OF VIRGINIA MCINTIRE | Charlottesville | B51Z Education | $39.6M | 7 |
| JACK KENT COOKE FOUNDATION | Lansdowne | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $39.3M | 11 |
| Mercatus Center Inc | Arlington | V22Z Social Science | $39.3M | 12 |
| DISTRIBUTIVE EDUCATION CLUBS OF AMERICA | Reston | B00Z Education | $39.3M | 49 |
| Southern Virginia University | Buena Vista | B41Z Education | $39.1M | 9 |
| CONQUER CANCER FOUNDATION OF THE | Alexandria | H113 Medical Research | $39.1M | 3 |
| COVENANT WOODS | Mechanicsville | E91 Health Care | $39.1M | 8 |
| NATIONAL ASSOCIATION OF STATE | Arlington | — | $38.9M | 7 |
| VOANS HOLDING COMPANY | Alexandria | — | $38.9M | 9 |
| WESTMINSTER-CANTERBURY OF LYNCHBURG INC | Lynchburg | P750 Human Services | $38.7M | 7 |
| NAVY SEAL FOUNDATION INC | Virginia Beach | A54 Arts, Culture & Humanities | $38.6M | 7 |
| Barbara Brunckhorst Foundation | Richmond | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $38.6M | 2 |
| Propane Education & Research Council | Richmond | — | $38.3M | 10 |
| RED GATES FOUNDATION | Richmond | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $38.2M | 0 |
| AIR FORCE RETIRED OFFICERS COMMUNITY | Potomac Falls | P750 Human Services | $38.2M | 5 |
| Navy Marine Coast Guard Residence Foundation | McLean | P20 Human Services | $38.2M | 9 |
| FOOD MARKETPLACE INC | Arlington | — | $38.1M | 14 |
| The Virginia Home | Richmond | E910 Health Care | $38.1M | 13 |
| AHC INC | Arlington | L20Z Housing & Shelter | $37.8M | 13 |
| THE MADEIRA SCHOOL | McLean | B200 Education | $37.6M | 10 |
| INFECTIOUS DISEASES SOCIETY OF AMERICA | Arlington | — | $37.6M | 14 |
| INSTITUTE FOR BUILDING TECHNOLOGY AND | Ashburn | W90 Public & Societal Benefit | $37.6M | 9 |
| CHRISTENDOM EDUCATIONAL CORPORATION | Front Royal | B400 Education | $37.1M | 7 |
| BLUE RIDGE HOSPICE INC | Winchester | P74Z Human Services | $37M | 11 |
| CHILDREN'S HEALTH SYSTEM INC | Norfolk | E110 Health Care | $36.8M | 12 |
| JEFFERSON SCHOLARS FOUNDATION | Charlottesville | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $36.6M | 10 |
| THE NORFOLK ACADEMY | Norfolk | B200 Education | $36.5M | 8 |
| AVIXA INC | Fairfax | — | $36.5M | 10 |
| INSURANCE INSTITUTE FOR HIGHWAY SAFETY | Arlington | M42Z Public Safety, Disaster Preparedness & Relief | $36.3M | 11 |
| NATIONAL DEFENSE INDUSTRIAL ASSOCIATION | Arlington | Q430 International, Foreign Affairs & National Security | $36M | 16 |
| Fort Norfolk Retirement Community Inc | Norfolk | P75 Human Services | $36M | 6 |
| COMMUNITIES IN SCHOOLS | Alexandria | B110 Education | $36M | 14 |
| Article IV | Arlington | W05 Public & Societal Benefit | $35.8M | 2 |
| PEDIATRIC SPECIALISTS OF VIRGINIA LLC | Fairfax | E30 Health Care | $35.7M | 13 |
| ASSOCIATION OF THE UNITED STATES ARMY | Arlington | W030 Public & Societal Benefit | $35.5M | 13 |
| RAPPAHANNOCK GOODWILL STORES AND | Fredericksburg | S11 Community Improvement & Capacity Building | $35.5M | 0 |
| Tax Analysts | Falls Church | — | $35.5M | 13 |
| NAMI National | Arlington | F80 Mental Health & Crisis Intervention | $35.3M | 8 |
| US Dairy Export Council | Arlington | — | $35.2M | 0 |
| Thomas Jefferson Foundation Inc | Charlottesville | A540 Arts, Culture & Humanities | $35M | 10 |
| HAMPTON ROADS SHIPPING ASSOCIATION ILA | Norfolk | — | $34.8M | 1 |
| VIRGINIA INNOVATION PARTNERSHIP | Herndon | U41Z Science & Technology | $34.8M | 10 |
| Consumer Brands Association | Arlington | — | $34.7M | 10 |
| Young America's Foundation | Reston | — | $34.4M | 15 |
| NRA FOUNDATION INC | Fairfax | I22Z Crime & Legal-Related | $34.2M | 5 |
| Virginia Museum of Fine Arts Foundation | Richmond | A112 Arts, Culture & Humanities | $34.2M | 7 |
| The Supplemental Sickness Benefit Plan | Arlington | — | $34.1M | 1 |
| ST ANNE'S-BELFIELD INC | Charlottesville | B200 Education | $34M | 7 |
| OLD DOMINION UNIVERSITY EDUCATIONAL | Norfolk | B110 Education | $33.8M | 0 |
| WESTMINSTER CANTERBURY OF WINCHESTER INC | Winchester | P750 Human Services | $33.8M | 8 |
