| Young America's Foundation | Reston | — | $41.6M | 11 |
| CONQUER CANCER FOUNDATION OF THE AMERICAN SOCIETY OF CLINICAL ONCOLOGY | Alexandria | H113 Medical Research | $41.4M | 3 |
| World Help | Forest | X99Z Religion-Related | $40.8M | 6 |
| NORTHERN VIRGINIA FAMILY SERVICE INC | Falls Church | P46Z Human Services | $40.6M | 10 |
| VALLEYSTAR CREDIT UNION | Martinsville | — | $40.6M | 34 |
| UNIVERSITY OF VIRGINIA FOUNDATION | Charlottesville | B110 Education | $40.4M | 12 |
| National Assn of Chain Drug Stores Inc | Arlington | — | $40.3M | 12 |
| IMAGINE SCHOOLS INC | Vienna | B20 Education | $40.2M | 11 |
| CHILDREN'S HEALTH SYSTEM INC | Norfolk | E110 Health Care | $40.1M | 15 |
| INSTITUTE FOR BUILDING TECHNOLOGY AND SAFETY | Ashburn | W90 Public & Societal Benefit | $40M | 9 |
| NATIONAL INDUSTRIES FOR THE BLIND | Alexandria | — | $40M | 13 |
| CLOSE UP FOUNDATION | Arlington | A30 Arts, Culture & Humanities | $39.9M | 12 |
| ST ANNE'S-BELFIELD INC | Charlottesville | B200 Education | $39.8M | 7 |
| INTERNET SOCIETY FOUNDATION | Reston | Q11 International, Foreign Affairs & National Security | $39.8M | 10 |
| Fort Norfolk Retirement Community Inc DBA Harbors Edge | Norfolk | P75 Human Services | $39.5M | 8 |
| JACK KENT COOKE FOUNDATION | Lansdowne | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $39.3M | 11 |
| Mercatus Center Inc | Arlington | V22Z Social Science | $39.3M | 12 |
| Southern Virginia University | Buena Vista | B41Z Education | $39.1M | 9 |
| COVENANT WOODS | Mechanicsville | E91 Health Care | $39.1M | 8 |
| CHARLOTTESVILLE AREA COMMUNITY FOUNDATION | Charlottesville | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $39M | 6 |
| WESTMINSTER CANTERBURY OF WINCHESTER INC | Winchester | P750 Human Services | $38.7M | 8 |
| WESTMINSTER-CANTERBURY OF LYNCHBURG INC | Lynchburg | P750 Human Services | $38.7M | 7 |
| NAVY SEAL FOUNDATION INC | Virginia Beach | A54 Arts, Culture & Humanities | $38.6M | 7 |
| Barbara Brunckhorst Foundation | Richmond | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $38.6M | 2 |
| PEDIATRIC SPECIALISTS OF VIRGINIA LLC | Fairfax | E30 Health Care | $38.4M | 13 |
| Propane Education & Research Council | Richmond | — | $38.3M | 10 |
| NATIONAL ASSOCIATION OF STATE DEPARTMENTS OF AGRICULTURE | Arlington | — | $38.2M | 7 |
| AIR FORCE RETIRED OFFICERS COMMUNITY - WASHINGTON DC | Potomac Falls | P750 Human Services | $38.2M | 5 |
| Navy Marine Coast Guard Residence Foundation | McLean | P20 Human Services | $38.2M | 9 |
| The Virginia Home | Richmond | E910 Health Care | $38.1M | 13 |
| AHC INC | Arlington | L20Z Housing & Shelter | $37.8M | 13 |
| CHRISTENDOM EDUCATIONAL CORPORATION | Front Royal | B400 Education | $37.6M | 7 |
| INFECTIOUS DISEASES SOCIETY OF AMERICA | Arlington | — | $37.6M | 14 |
| BLUE RIDGE HOSPICE INC | Winchester | P74Z Human Services | $37M | 11 |
| The Madeira School | McLean | B200 Education | $36.8M | 11 |
| JEFFERSON SCHOLARS FOUNDATION | Charlottesville | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $36.6M | 10 |
| THE NORFOLK ACADEMY | Norfolk | B200 Education | $36.5M | 8 |
| INSURANCE INSTITUTE FOR HIGHWAY SAFETY | Arlington | M42Z Public Safety, Disaster Preparedness & Relief | $36.3M | 11 |
| NATIONAL DEFENSE INDUSTRIAL ASSOCIATION | Arlington | Q430 International, Foreign Affairs & National Security | $36M | 16 |
| GREGG TURK FOUNDATION | Reston | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $35.7M | 0 |
| Tax Analysts | Falls Church | — | $35.5M | 13 |
| JOHNSON HEALTH CENTER | Madison Heights | E39Z Health Care | $35.3M | 12 |
| NAMI National | Arlington | F80 Mental Health & Crisis Intervention | $35.3M | 8 |
| US Dairy Export Council | Arlington | — | $35.2M | 0 |
| LONGWOOD UNIVERSITY REAL ESTATE FOUNDATION | Farmville | B90 Education | $35.1M | 2 |
| Thomas Jefferson Foundation Inc | Charlottesville | A540 Arts, Culture & Humanities | $35M | 10 |
| HAMPTON ROADS SHIPPING ASSOCIATION ILA CONTAINER ROYALTY FUND | Norfolk | — | $34.8M | 1 |
| Consumer Brands Association | Arlington | — | $34.7M | 10 |
| Virginia Museum of Fine Arts Foundation | Richmond | A112 Arts, Culture & Humanities | $34.2M | 7 |
| The Supplemental Sickness Benefit Plan Covering Railroad Shop Craft and Signal | Arlington | — | $34.1M | 1 |
