| JOHNS HOPKINS UNIVERSITY | Baltimore | B430 Education | $10.2B | 31 |
| THE JOHNS HOPKINS HOSPITAL | Baltimore | E220 Health Care | $3.2B | 20 |
| A D F COMMUNITY OUTREACH FOUNDATION INC | Mechanicsville | T50 Philanthropy, Voluntarism & Grantmaking Foundations | $2.7B | 0 |
| University of Maryland Medical System Corporation | Baltimore | E220 Health Care | $2.2B | 19 |
| HH MEDSTAR HEALTH INC | Columbia | E210 Health Care | $1.9B | 12 |
| Howard Hughes Medical Institute | Chevy Chase | H920 Medical Research | $1.8B | 33 |
| Financial Industry Regulatory Authority Inc | Rockville | — | $1.6B | 36 |
| FINRA REGULATION INC | Rockville | — | $1.4B | 8 |
| AMERICAN POSTAL WORKERS UNION AFL CIO HEALTH | Elkridge | — | $1.3B | 24 |
| SINAI HOSPITAL OF BALTIMORE INC | Baltimore | E220 Health Care | $1B | 16 |
| ADVENTIST HEALTHCARE INC | Gaithersburg | — | $910.2M | 21 |
| IMPACTASSETSINC | Bethesda | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $866.5M | 7 |
| JOHNS HOPKINS BAYVIEW | Baltimore | E220 Health Care | $767.2M | 11 |
| HEALTHWELL FOUNDATION | Germantown | E99 Health Care | $742.4M | 19 |
| JOHNS HOPKINS HEALTH SYSTEM CORPORATION | Baltimore | E210 Health Care | $732.7M | 47 |
| NECA IBEW FAMILY MEDICAL CARE TRUST FUND | Rockville | Y43 Mutual & Membership Benefit | $712.9M | 2 |
| JOHNS HOPKINS ALL CHILDREN'S HOSPITAL | Baltimore | E240 Health Care | $702.2M | 24 |
| JACK AND NANCY DWYER WORKFORCE | Baltimore | E91 Health Care | $696.1M | 6 |
| BOARD OF EDUCATION OF MONTGOMERY COUNTY EMPLOYEE BENEFIT TRUST FUND | Rockville | — | $690.1M | 0 |
| HOLY CROSS HEALTH INC | Silver Spring | — | $681.6M | 14 |
| LUMINIS HEALTH ANNE ARUNDEL MEDICAL | Annapolis | E220 Health Care | $652.5M | 16 |
| Mercy Medical Center | Baltimore | E220 Health Care | $633.9M | 15 |
| HENRY M JACKSON FOUNDATION FOR THE ADVANCEMENT OF MILITARY MEDICINE | Bethesda | H120 Medical Research | $628.9M | 13 |
| GREATER BALTIMORE MEDICAL CENTER INC | Baltimore | E220 Health Care | $624.7M | 18 |
| FRANKLIN SQUARE HOSPITAL CENTER INC | Baltimore | E220 Health Care | $564.1M | 13 |
| MERITUS MEDICAL CENTER INC | Hagerstown | E220 Health Care | $563.4M | 14 |
| SOUTHERN MARYLAND | Hughesville | W80Z Public & Societal Benefit | $542.5M | 28 |
| UMSJ Health System LLC | Towson | E11 Health Care | $537.5M | 15 |
| TIDALHEALTH PENINSULA REGIONAL INC | Salisbury | E220 Health Care | $527.7M | 14 |
| JOHNS HOPKINS MEDICAL | Baltimore | E220 Health Care | $476.5M | 4 |
| Baltimore Washington Medical Center Inc | Glen Burnie | E220 Health Care | $465.1M | 14 |
| THE UNION MEMORIAL HOSPITAL | Baltimore | E220 Health Care | $439.4M | 20 |
| Dimensions Health Corporation | Upper Marlboro | E220 Health Care | $436.1M | 17 |
| JHPIEGO CORPORATION | Baltimore | — | $421.3M | 17 |
| FREDERICK HEALTH HOSPITAL INC | Frederick | E220 Health Care | $408.2M | 26 |
| UPMC WESTERN MARYLAND CORPORATION | Cumberland | E20 Health Care | $398.9M | 11 |
| NATIONAL AUTOMATIC SPRINKLER | Landover | — | $385.6M | 13 |
| LOYOLA UNIVERSITY MARYLAND INC | Baltimore | — | $368.2M | 16 |
| MEDSTAR HEALTH INC | Columbia | E210 Health Care | $366.7M | 15 |
| SUBURBAN HOSPITAL INC | Bethesda | E220 Health Care | $366.4M | 13 |
| UNITED STATES PHARMACOPEIAL CONVENTION | Rockville | E050 Health Care | $353.7M | 14 |
| Upper Chesapeake Medical Center Inc | Bel Air | E220 Health Care | $333.5M | 12 |
| NFL PLAYER DISABILITY & SURVIVOR BENEFIT | Baltimore | — | $324M | 0 |
| HOWARD COUNTY GENERAL HOSPITAL INC | Baltimore | E220 Health Care | $319.9M | 10 |
| LIFEBRIDGE HEALTH INC | Baltimore | E210 Health Care | $317.4M | 17 |
| National Gallery of Art | Landover | A510 Arts, Culture & Humanities | $315.5M | 18 |
| JOHNS HOPKINS COMMUNITY PHYSICIANS INC | Baltimore | — | $309.4M | 10 |
| NORTHWEST HOSPITAL CENTER INC | Randallstown | E22 Health Care | $308.7M | 16 |
| AMERICAN KIDNEY FUND INC | Rockville | — | $305M | 7 |
| LUTHERAN IMMIGRATION & REFUGEE SERVICE | Baltimore | — | $304.3M | 0 |
