| AARP | Washington | R25 Civil Rights, Social Action & Advocacy | $11B | 20 |
| AMERICAN NATIONAL RED CROSS & ITS CONSTITUENT CHAPTERS AND BRANCHES | Washington | P210 Human Services | $3.8B | 16 |
| GEORGETOWN UNIVERSITY | Washington | B430 Education | $2.1B | 12 |
| NATIONAL ASSOCIATION OF LETTER CARRIERS | Washington | J40 Employment | $2B | 69 |
| SMITHSONIAN INSTITUTION | Washington | A50Z Arts, Culture & Humanities | $2B | 27 |
| CHILDREN'S HOSPITAL | Washington | E240 Health Care | $1.7B | 23 |
| WASHINGTON HOSPITAL CENTER CORPORATION | Washington | E210 Health Care | $1.7B | 13 |
| THE MEDSTAR-GEORGETOWN MEDICAL CENTER INC | Washington | E220 Health Care | $1.7B | 16 |
| THE HOWARD UNIVERSITY | Washington | B430 Education | $1.4B | 21 |
| AMERICAN UNIVERSITY | Washington | B43 Education | $937.1M | 20 |
| AMERICAN CHEMICAL SOCIETY | Washington | U330 Science & Technology | $819.1M | 12 |
| PAYPAL CHARITABLE GIVING FUND | Washington | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $813.4M | 2 |
| AMERICAN FOREIGN SERVICE PROTECTIVE ASSOCIATION | Washington | — | $687.3M | 9 |
| NEW VENTURE FUND | Washington | C05 Environment | $662.4M | 7 |
| LUCY WEBB HAYES NATIONAL TRAINING SCHOOL FOR DEACONESSES & MISSIONARIES | Washington | E220 Health Care | $653.7M | 16 |
| CORPORATION FOR PUBLIC BROADCASTING | Washington | A300 Arts, Culture & Humanities | $640.1M | 21 |
| SECURITIES INVESTOR PROTECTION CORPORATION | Washington | — | $625.3M | 14 |
| Fiat Lux Risk and Insurance Company | Washington | Y20 Mutual & Membership Benefit | $621.3M | 7 |
| FUTURE FORWARD USA ACTION | Washington | W05 Public & Societal Benefit | $615.3M | 10 |
| LEGAL SERVICES CORPORATION | Washington | I800 Crime & Legal-Related | $577.1M | 21 |
| United Mine Workers of America 1993 Benefit Plan | Washington | — | $541.6M | 4 |
| PHARMACEUTICAL RESEARCH AND MANUFACTURERS OF AMERICA | Washington | — | $521.3M | 15 |
| NATIONAL FISH AND WILDLIFE FOUNDATION | Washington | D300 Animal-Related | $513.2M | 11 |
| ABAX VENTURES INC | Washington | W99 Public & Societal Benefit | $482.1M | 0 |
| ASSOCIATION OF UNIVERSITIES FOR RESEARCH IN ASTRONOMY INC | Washington | U31C Science & Technology | $450.4M | 23 |
| THE ASPEN INSTITUTE INC | Washington | V200 Social Science | $443.8M | 12 |
| The Pew Charitable Trusts | Washington | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $408.9M | 11 |
| Catholic University of America | Washington | B430 Education | $408.7M | 15 |
| NATIONAL EDUCATION ASSOCIATION OF THE UNITED STATES | Washington | — | $402.8M | 81 |
| MEDICAL FACULTY ASSOCIATES INC | Washington | E300 Health Care | $389.5M | 21 |
| WORLD WILDLIFE FUND INC | Washington | C30 Environment | $374.8M | 13 |
| THE LEUKEMIA & LYMPHOMA SOCIETYINC | Washington | G300 Diseases, Disorders & Medical Disciplines | $368.2M | 11 |
| LABORERS-EMPLOYERS BENEFIT PLAN COLLECTION TRUST | Washington | — | $364.8M | 1 |
| NATIONAL ENDOWMENT FOR DEMOCRACY | Washington | W010 Public & Societal Benefit | $356.2M | 8 |
| WORLD CENTRAL KITCHEN INC | Washington | Q33 International, Foreign Affairs & National Security | $327.8M | 13 |
| NATIONAL PUBLIC RADIO INC | Washington | A340 Arts, Culture & Humanities | $325.2M | 27 |
| NATIONAL ACADEMY OF SCIENCES | Washington | U03 Science & Technology | $324.6M | 17 |
| WINDWARD FUND | Washington | C30 Environment | $308.2M | 6 |
| JOHN F KENNEDY CENTER FOR THE PERFORMING ARTS | Washington | A610 Arts, Culture & Humanities | $306.9M | 18 |
| POPULATION SERVICES INTERNATIONAL | Washington | — | $306.3M | 14 |
| ASSOCIATION OF AMERICAN MEDICAL COLLEGES | Washington | B030 Education | $303.7M | 20 |
| SERVICE EMPLOYEES INTERNATIONAL UNION | Washington | — | $280.8M | 18 |
| NATIONAL PARK FOUNDATION | Washington | — | $275.1M | 13 |
| Amalgamated Charitable Foundation Inc | Washington | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $273.8M | 0 |
| INTERNATIONAL CITY MANAGEMENT ASSOCIATION RETIREMENT CORPORATION | Washington | Y30 Mutual & Membership Benefit | $269M | 15 |
| WORLD RESOURCES INSTITUTE | Washington | Q055 International, Foreign Affairs & National Security | $267.4M | 17 |
| AMERICAN PETROLEUM INSTITUTE | Washington | — | $252.3M | 14 |
| UNITED NEGRO COLLEGE FUND INC | Washington | B820 Education | $250.9M | 15 |
| INTERNATIONAL BACCALAUREATE ORGANIZATION | Washington | B90 Education | $250.3M | 28 |
| UNITED FOOD AND COMMERCIAL WORKERS INTERNATIONAL UNION | Washington | J40 Employment | $244.2M | 65 |
