| INOVA HEALTH CARE SERVICES | Fairfax | E200 Health Care | $5.7B | 19 |
| SENTARA HEALTH PLANS | Virginia Beach | E310 Health Care | $5.2B | 11 |
| SENTARA HOSPITALS | Virginia Beach | E200 Health Care | $4B | 25 |
| GOOD360 | Alexandria | Q193 International, Foreign Affairs & National Security | $3.3B | 20 |
| THE MITRE CORPORATION | McLean | U400 Science & Technology | $2.4B | 48 |
| Riverside Healthcare Association Inc Group | Newport News | — | $2.3B | 88 |
| NATIONAL RURAL UTILITIES COOPERATIVE FINANCE CORPORATION | Dulles | — | $2.1B | 46 |
| Carilion Medical Center | Roanoke | E20 Health Care | $2B | 22 |
| THE GEORGE WASHINGTON UNIVERSITY | Ashburn | B430 Education | $1.9B | 20 |
| LIBERTY UNIVERSITY INC | Lynchburg | B430 Education | $1.8B | 45 |
| The Railroad Employees National Health & Welfare Plan | Arlington | — | $1.8B | 0 |
| THE NATURE CONSERVANCY | Arlington | — | $1.5B | 17 |
| VIRGINIA PREMIER HEALTH PLAN INC | Virginia Beach | — | $1.5B | 17 |
| VALLEY HEALTH SYSTEM GROUP RETURN | Winchester | — | $1.4B | 42 |
| The Aerospace Corporation | Chantilly | U300 Science & Technology | $1.4B | 42 |
| SENTARA HEALTH | Virginia Beach | E210 Health Care | $1.3B | 31 |
| OLD DOMINION ELECTRIC COOPERATIVE | Glen Allen | — | $1.1B | 25 |
| CENTRA HEALTH INC | Lynchburg | E220 Health Care | $1.1B | 17 |
| MARY WASHINGTON HEALTHCARE GROUP RETURN | Fredericksburg | E22 Health Care | $1.1B | 31 |
| CHARITIES AID FOUNDATION AMERICA | Alexandria | T30Z Philanthropy, Voluntarism & Grantmaking Foundations | $951.9M | 9 |
| UNIVERSITY OF VIRGINIA INVESTMENT MANAGEMENT COMPANY | Charlottesville | B11 Education | $912.3M | 16 |
| Virginia Hospital Center Arlington Health System | Arlington | E210 Health Care | $896.4M | 20 |
| INOVA HEALTH SYSTEM FOUNDATION | Fairfax | E110 Health Care | $885.9M | 14 |
| NORTHERN VIRGINIA ELECTRIC CO-OP | Manassas | — | $875.4M | 27 |
| NRECA GROUP BENEFITS TRUST | Arlington | — | $838.3M | 7 |
| DELTA DENTAL OF VIRGINIA | Roanoke | E31 Health Care | $807.5M | 17 |
| SMART - TD HEALTH AND WELFARE PLAN | Arlington | Y43 Mutual & Membership Benefit | $792.4M | 0 |
| AVMED INC | Virginia Beach | — | $698.7M | 16 |
| CHILDREN'S HOSPITAL OF THE KING'S DAUGHTERS | Norfolk | E240 Health Care | $665.8M | 18 |
| University of Virginia Physicians Group | Charlottesville | E112 Health Care | $656.3M | 25 |
| MCV Associated Physicians | Richmond | E30 Health Care | $632.3M | 17 |
| NOBLIS INC | Reston | U200 Science & Technology | $621.3M | 27 |
| RAPPAHANNOCK ELECTRIC COOPERATIVE | Fredericksburg | — | $605.7M | 26 |
| LIFENET HEALTH | Virginia Beach | E65 Health Care | $598.6M | 20 |
| SENTARA RMH MEDICAL CENTER | Virginia Beach | E220 Health Care | $575.2M | 18 |
| AUGUSTA HEALTH CARE INC | Fishersville | E220 Health Care | $559.1M | 23 |
| PUBLIC BROADCASTING SERVICE | Arlington | A320 Arts, Culture & Humanities | $558.2M | 15 |
| BAE SYSTEMS WELFARE BENEFIT TRUST | Falls Church | Y43 Mutual & Membership Benefit | $534.2M | 1 |
| MARINE TOYS FOR TOTS FOUNDATION | Triangle | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $506.1M | 8 |
| UNIVERSITY OF RICHMOND | Richmond | B430 Education | $498.3M | 16 |
| American Foundation for Donation and | Richmond | E65Z Health Care | $479.5M | 2 |
| CFA INSTITUTE | Charlottesville | — | $462.9M | 19 |
| Stand Together C4 Fund | Arlington | W01 Public & Societal Benefit | $452.1M | 3 |
| AMERICAN INSTITUTES FOR RESEARCH IN THE BEHAVIORAL SCIENCES | Arlington | V230 Social Science | $437.2M | 31 |
| STAND TOGETHER CHAMBER OF COMMERCE INC | Arlington | — | $432.6M | 12 |
| Carilion Services Inc | Roanoke | E210 Health Care | $427.1M | 17 |
| NRLCA VEBA TRUST | Alexandria | P43 Human Services | $424.6M | 2 |
| PARTNERSHIP FOR SUPPLY CHAIN MANAGEMENT | Arlington | P20 Human Services | $415.7M | 11 |
| SENTARA PRINCESS ANNE HOSPITAL | Virginia Beach | E20 Health Care | $409.1M | 15 |
| MARTHA JEFFERSON HOSPITAL | Virginia Beach | E220 Health Care | $406.6M | 18 |
