| LUTHERAN SOCIAL SERVICES OF COLORADO | Denver | — | $35.5M | 6 |
| THE WELD TRUST | Greeley | E11 Health Care | $35.2M | 6 |
| IMPACT DEVELOPMENT FUND | Loveland | L120 Housing & Shelter | $35.2M | 8 |
| WATER FOR PEOPLE | Greenwood Village | Q30Z International, Foreign Affairs & National Security | $35M | 10 |
| San Miguel Power Association Inc | Nucla | — | $35M | 15 |
| Karuna Foundation | Niwot | D01 Animal-Related | $34.5M | 0 |
| WINDSOR CHARTER ACADEMY | Windsor | B29 Education | $33.7M | 6 |
| PROLOGIS FOUNDATION | Denver | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $33.6M | 0 |
| EL POMAR FOUNDATION | Colorado Springs | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $33.6M | 16 |
| COLORADO OPEN LANDS | Lakewood | C340 Environment | $33.1M | 1 |
| MERIDIAN INSTITUTE | Dillon | T20I Philanthropy, Voluntarism & Grantmaking Foundations | $33.1M | 11 |
| COMMUNITY FOOD SHARE INC | Louisville | K300 Food, Agriculture & Nutrition | $33M | 0 |
| Valley Citizens Foundation for Health | Del Norte | E20Z Health Care | $32.7M | 7 |
| Augustine Institute Inc | Greenwood Village | B50 Education | $32.5M | 12 |
| KENT DENVER COUNTRY DAY SCHOOL | Englewood | B250 Education | $32.3M | 8 |
| The Food Bank for Larimer County | Loveland | K31Z Food, Agriculture & Nutrition | $32.2M | 2 |
| ASPEN GLOBAL CHANGE INSTITUTE INC | Basalt | B60 Education | $32.1M | 3 |
| COLORADO AFFORDABLE CATHOLIC | Denver | L120 Housing & Shelter | $32M | 4 |
| Naropa University | Boulder | — | $31.9M | 7 |
| BEHAVIOR ANALYST CERTIFICATION BOARD | Littleton | J20 Employment | $31.7M | 6 |
| COLORADO WEST REGIONAL MENTAL HEALTH | Grand Junction | F32 Mental Health & Crisis Intervention | $31.6M | 0 |
| GATES FAMILY FOUNDATION | Denver | — | $31.6M | 2 |
| LAS ANIMAS COUNTY REHABILITATION | Trinidad | P82 Human Services | $31.6M | 2 |
| KC Electric Association Inc | Hugo | — | $31.5M | 14 |
| HIGHLANDS RANCH COMMUNITY ASSOCIATION | Highlands Ranch | L502 Housing & Shelter | $31.3M | 8 |
| BROTHERS REDEVELOPMENT INC | Edgewater | L20Z Housing & Shelter | $31.2M | 1 |
| FUJITSU GROUP HEALTH BENEFIT PLAN TRUST | Denver | — | $31.2M | 1 |
| United States Anti-Doping Agency | Colorado Springs | N71 Recreation & Sports | $30.8M | 23 |
| THE CONSCIOUS ALLIANCE | Broomfield | K30 Food, Agriculture & Nutrition | $30.8M | 1 |
| The Civic Problem-Solving Endowment Inc | Denver | W99 Public & Societal Benefit | $30.6M | 2 |
| JAZZ AT ASPEN-SNOWMASS | Aspen | A680 Arts, Culture & Humanities | $30.4M | 4 |
| BEAVER CREEK RESORT COMPANY OF COLORADO | Avon | — | $29.5M | 1 |
| ARIEL CLINICAL SERVICES | Grand Junction | P70Z Human Services | $29.5M | 5 |
| ICAST | Lakewood | S32 Community Improvement & Capacity Building | $29.4M | 1 |
| Chavez Huerta K-12 Preparatory Academy | Pueblo | B29 Education | $29M | 2 |
| NORTH METRO COMMUNITY SERVICES | Westminster | P700 Human Services | $28.9M | 7 |
| INTERNATIONAL ASSOCIATION FOR THE | Denver | S41 Community Improvement & Capacity Building | $28.6M | 11 |
| Third Future Schools | Aurora | B29 Education | $28.6M | 8 |
| MENTAL HEALTH CENTER OF BOULDER COUNTY | Lafayette | — | $28.5M | 8 |
| The Colorado Trust | Denver | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $28.4M | 14 |
| THE COLORADO BOARDS OF EDUCATION | Greenwood Village | Y44 Mutual & Membership Benefit | $28.3M | 2 |
| EAGLE VALLEY MENTAL HEALTH | Vail | E60 Health Care | $28.2M | 4 |
| TOASTMASTERS INTERNATIONAL | Englewood | N500 Recreation & Sports | $27.9M | 13 |
| INTERVENTION INC | Lakewood | I41Z Crime & Legal-Related | $27.9M | 7 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION | Colorado Springs | P270 Human Services | $27.6M | 7 |
| ACADEMY OF CHARTER SCHOOLS INC | Westminster | B20 Education | $27.5M | 3 |
| THE FORT LEWIS COLLEGE FOUNDATION | Durango | B42I Education | $27.4M | 0 |
| MUSIC ASSOCIATES OF ASPEN INC | Aspen | A6E0 Arts, Culture & Humanities | $27.3M | 17 |
| ISAIAH 5812 | Parker | X01 Religion-Related | $27M | 0 |
| ROCKY MOUNTAIN PUBLIC MEDIA INC | Denver | A320 Arts, Culture & Humanities | $27M | 7 |
