| NORTHWEST COLORADO VISITING NURSE ASSOCIATION | Steamboat Springs | E920 Health Care | $37.3M | 8 |
| ROCKY MOUNTAIN PREPARATORY SCHOOLS | Denver | — | $37.1M | 9 |
| Naropa University | Boulder | — | $36.9M | 10 |
| DIVERSUS HEALTH INC | Colorado Springs | E210 Health Care | $36.6M | 9 |
| COLORADO HEALTH NETWORK INC | Denver | G81Z Diseases, Disorders & Medical Disciplines | $36.5M | 6 |
| THE WILD ANIMAL SANCTUARY | Keenesburg | D340 Animal-Related | $36.4M | 5 |
| THE MYRON STRATTON HOME | Colorado Springs | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $36.2M | 6 |
| JUNIOR ACHIEVEMENT USA | Colorado Springs | O53 Youth Development | $35.8M | 15 |
| HABITAT FOR HUMANITY OF METRO DENVER INC | Denver | — | $35.8M | 8 |
| BSLC II | Colorado Springs | P75 Human Services | $35.8M | 16 |
| LUTHERAN SOCIAL SERVICES OF COLORADO | Denver | — | $35.5M | 6 |
| Karuna Foundation | Niwot | D01 Animal-Related | $35.4M | 0 |
| ASSOCIATION OF OPERATING ROOM NURSES INC | Denver | — | $35.3M | 11 |
| THE WELD TRUST | Greeley | E11 Health Care | $35.2M | 6 |
| IMPACT DEVELOPMENT FUND | Loveland | L120 Housing & Shelter | $35.2M | 8 |
| WATER FOR PEOPLE | Greenwood Village | Q30Z International, Foreign Affairs & National Security | $35M | 10 |
| San Miguel Power Association Inc | Nucla | — | $35M | 15 |
| COMMUNITY REACH CENTER INC | Westminster | E60 Health Care | $34.2M | 9 |
| WINDSOR CHARTER ACADEMY | Windsor | B29 Education | $33.7M | 6 |
| EL POMAR FOUNDATION | Colorado Springs | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $33.6M | 16 |
| FLATIRON CONSTRUCTION CORP EMPLOYEE HEALTH BENEFIT TRUST | Broomfield | — | $33.2M | 3 |
| COLORADO OPEN LANDS | Lakewood | C340 Environment | $33.1M | 1 |
| Chavez Huerta K-12 Preparatory Academy | Pueblo | B29 Education | $33.1M | 1 |
| COMMUNITY FOOD SHARE INC | Louisville | K300 Food, Agriculture & Nutrition | $33M | 0 |
| Valley Citizens Foundation for Health Care Inc | Del Norte | E20Z Health Care | $32.7M | 7 |
| COMMUNITY FOUNDATION OF NORTHERN COLORADO | Fort Collins | T31Z Philanthropy, Voluntarism & Grantmaking Foundations | $32.4M | 4 |
| Contractors Health Trust FKA Colorado | Englewood | — | $32.3M | 3 |
| HIGHLANDS RANCH COMMUNITY ASSOCIATION INC | Highlands Ranch | L502 Housing & Shelter | $32.2M | 8 |
| The Food Bank for Larimer County | Loveland | K31Z Food, Agriculture & Nutrition | $32.2M | 2 |
| ASPEN GLOBAL CHANGE INSTITUTE INC | Basalt | B60 Education | $32.1M | 3 |
| BEHAVIOR ANALYST CERTIFICATION BOARD INC | Littleton | J20 Employment | $31.7M | 6 |
| COLORADO WEST REGIONAL MENTAL HEALTH INC | Grand Junction | F32 Mental Health & Crisis Intervention | $31.6M | 0 |
| GATES FAMILY FOUNDATION | Denver | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $31.6M | 2 |
| KC Electric Association Inc | Hugo | — | $31.5M | 14 |
| BROTHERS REDEVELOPMENT INC | Edgewater | L20Z Housing & Shelter | $31.2M | 1 |
| CATENA FOUNDATION | Carbondale | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $31.2M | 1 |
| FUJITSU GROUP HEALTH BENEFIT PLAN TRUST C/O US BANK TTEE | Denver | — | $31.2M | 1 |
| NORTH METRO COMMUNITY SERVICES | Westminster | P700 Human Services | $31M | 9 |
| THE CONSCIOUS ALLIANCE C/O JUSTIN LEVY | Broomfield | K30 Food, Agriculture & Nutrition | $30.8M | 1 |
| The Civic Problem-Solving Endowment Inc | Denver | W99 Public & Societal Benefit | $30.6M | 2 |
| JAZZ AT ASPEN-SNOWMASS | Aspen | A680 Arts, Culture & Humanities | $30.4M | 4 |
| BEAVER CREEK RESORT COMPANY OF COLORADO | Avon | — | $29.5M | 1 |
| ARIEL CLINICAL SERVICES | Grand Junction | P70Z Human Services | $29.5M | 5 |
| ICAST | Lakewood | S32 Community Improvement & Capacity Building | $29.4M | 1 |
| RICHMOND AMERICAN FOUNDATION | Greenwood Village | T11 Philanthropy, Voluntarism & Grantmaking Foundations | $28.8M | 0 |
| INTERNATIONAL ASSOCIATION FOR THE STUDY OF LUNG CANCER | Denver | S41 Community Improvement & Capacity Building | $28.6M | 11 |
| Third Future Schools | Aurora | B29 Education | $28.6M | 8 |
| MENTAL HEALTH CENTER OF BOULDER COUNTY INC | Lafayette | — | $28.5M | 8 |
| The Colorado Trust | Denver | T21 Philanthropy, Voluntarism & Grantmaking Foundations | $28.4M | 14 |
| United States Space Foundation | Colorado Springs | B90 Education | $28.3M | 12 |
