| EAGLE VALLEY MENTAL HEALTH | Vail | E60 Health Care | $28.2M | 4 |
| TOASTMASTERS INTERNATIONAL | Englewood | N500 Recreation & Sports | $27.9M | 13 |
| INTERVENTION INC | Lakewood | I41Z Crime & Legal-Related | $27.9M | 7 |
| GLENMOOR COUNTRY CLUB | Cherry Hills Village | — | $27.9M | 8 |
| SMITH AGENCY | Aurora | P80B Human Services | $27.8M | 1 |
| Medical Education Resources Inc | Englewood | — | $27.8M | 3 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF THE PIKES PEAK REGION | Colorado Springs | P270 Human Services | $27.6M | 7 |
| ACADEMY OF CHARTER SCHOOLS INC | Westminster | B20 Education | $27.5M | 3 |
| THE FORT LEWIS COLLEGE FOUNDATION | Durango | B42I Education | $27.4M | 0 |
| GRALAND COUNTRY DAY SCHOOL | Denver | B200 Education | $27.4M | 7 |
| PEAK TO PEAK CHARTER SCHOOLS INC | Lafayette | B20 Education | $27.4M | 8 |
| ISAIAH 5812 | Parker | X01 Religion-Related | $27M | 0 |
| PUBLIC BROADCASTING OF COLORADO INC | Centennial | A340 Arts, Culture & Humanities | $27M | 11 |
| YAMPA VALLEY COMMUNITY FOUNDATION | Steamboat Springs | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $26.9M | 2 |
| James Irwin Charter Schools | Colorado Springs | B29 Education | $26.8M | 2 |
| PROSPECT RIDGE ACADEMY | Broomfield | B29 Education | $26.6M | 5 |
| Via Mobility Services | Boulder | P520 Human Services | $26.1M | 2 |
| Sangre de Cristo Electric Association Inc | Buena Vista | — | $26.1M | 17 |
| PARTNER COLORADO CREDIT UNION | Arvada | — | $25.9M | 22 |
| EDUCAUSE | Boulder | B600 Education | $25.7M | 11 |
| COLORADO CREDIT UNION | Littleton | — | $25.7M | 24 |
| COVENANT LIVING OF COLORADO INC | Westminster | P750 Human Services | $25.4M | 18 |
| David Merage Foundation for Confronting Antisemitism | Englewood | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $25.4M | 0 |
| WESTERN OPPORTUNITY FUND | Denver | B90 Education | $25.1M | 0 |
| Community Partnership for Child Development | Colorado Springs | B012 Education | $25.1M | 4 |
| UNITED STATES CENTER FOR SAFESPORT | Denver | N03 Recreation & Sports | $25M | 15 |
| COLORADO LEGAL SERVICES | Denver | I80 Crime & Legal-Related | $25M | 11 |
| UNITED STATES FIGURE SKATING ASSOCIATION | Colorado Spring | — | $24.9M | 8 |
| LARADON HALL SOCIETY FOR EXCEPTIONAL CHILDREN AND ADULTS | Denver | P700 Human Services | $24.9M | 6 |
| NATIVE AMERICAN RIGHTS FUND INC | Boulder | R220 Civil Rights, Social Action & Advocacy | $24.6M | 10 |
| PIKES PEAK HOSPICE AND PALLIATIVE CARE INC | Denver | — | $24.5M | 9 |
| WEDONTWASTE INC | Denver | K30 Food, Agriculture & Nutrition | $24.5M | 3 |
| IMPACT CHARITABLE | Denver | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $24.5M | 6 |
| NATIONAL STRENGTH AND CONDITIONING ASSOC | Colorado Springs | N40 Recreation & Sports | $24.4M | 11 |
| MOUNTAIN FAMILY HEALTH CENTERS | Glenwood Springs | E32Z Health Care | $24.4M | 11 |
| UNITED STATES OF AMERICA WRESTLING | Colorado Springs | N6XZ Recreation & Sports | $24.3M | 17 |
| WELD FOOD BANK | Greeley | K31Z Food, Agriculture & Nutrition | $24.3M | 2 |
| Atlas Preparatory School Inc | Colorado Springs | B29 Education | $24.3M | 4 |
| THE WATER RESEARCH FOUNDATION | Denver | C32 Environment | $24.1M | 9 |
| ROCKY MOUNTAIN MUTUAL HOUSING ASSOCIATION INC | Denver | L210 Housing & Shelter | $24.1M | 2 |
| COLORADO ENTERPRISE FUND | Denver | S31Z Community Improvement & Capacity Building | $24M | 9 |
| BOETTCHER FOUNDATION | Denver | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $24M | 11 |
| CREDIT UNIONS CHARTERED IN STATE OF CO 85 ON TAP CREDIT UNION | Golden | — | $23.9M | 19 |
| PATHWAYS HOSPICE | Denver | P74Z Human Services | $23.9M | 10 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF METROPOLITAN DENVER | Denver | P270 Human Services | $23.8M | 6 |
| ROCKY MOUNTAIN PUBLIC MEDIA INC | Denver | A320 Arts, Culture & Humanities | $23.5M | 7 |
| BREWERS ASSOCIATION INC | Boulder | — | $23.4M | 9 |
| United States Anti-Doping Agency | Colorado Springs | N71 Recreation & Sports | $23.3M | 21 |
| Find Us Faithful Foundation | Parker | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $23.3M | 3 |
| GUNNISON COUNTY ELECTRIC ASSOCIATION | Gunnison | — | $23.3M | 16 |
