| Towle Family Foundation | Greenwood Village | T90Z Philanthropy, Voluntarism & Grantmaking Foundations | $46.6M | 0 |
| NATIONAL TRUST FOR LOCAL NEWS | Englewood | A30 Arts, Culture & Humanities | $46.1M | 6 |
| KIPP Colorado Schools | Denver | B29 Education | $45.7M | 9 |
| MORGAN COUNTY RURAL ELECTRIC ASSOCIATION | Fort Morgan | — | $45.4M | 17 |
| COMMUNITY DEVELOPMENT INSTITUTE | Denver | B21 Education | $44.8M | 7 |
| PIPE INDUSTRY HEALTH AND WELFARE | Denver | — | $44.8M | 1 |
| NORTH RANGE BEHAVIORAL HEALTH | Greeley | F31Z Mental Health & Crisis Intervention | $44.6M | 12 |
| THE WESTERN STOCK SHOW ASSOCIATION | Denver | N520 Recreation & Sports | $44.5M | 6 |
| Y W Electric Association Inc | Akron | — | $44.5M | 14 |
| SAN LUIS VALLEY RURAL ELECTRIC COOPERATIVE INC | Monte Vista | — | $44.3M | 19 |
| MOUNTAIN PARKS ELECTRIC INC | Granby | — | $43.7M | 21 |
| DIVERSUS HEALTH INC | Colorado Springs | E210 Health Care | $43.6M | 9 |
| MILE HIGH UNITED WAY INC | Denver | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $43.4M | 7 |
| INTERNATIONAL DEVELOPMENT ENTERPRISES | Denver | Q023 International, Foreign Affairs & National Security | $43.4M | 10 |
| HEALTH SOLUTIONS | Pueblo | F32Z Mental Health & Crisis Intervention | $43.2M | 17 |
| USA VOLLEYBALL | Colorado Springs | N60 Recreation & Sports | $42.8M | 16 |
| CHEYENNE MOUNTAIN ZOOLOGICAL | Colorado Springs | D500 Animal-Related | $42.8M | 9 |
| GLOBAL GREENGRANTS FUND | Boulder | C12 Environment | $42.6M | 8 |
| SOOPER CREDIT UNION | Arvada | — | $42.6M | 25 |
| NURSE-FAMILY PARTNERSHIP | Denver | E80 Health Care | $42.5M | 11 |
| INSTITUTE FOR SHIPBOARD EDUCATION | Fort Collins | B400 Education | $42.5M | 7 |
| Rose Community Foundation | Denver | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $42.2M | 7 |
| HILLTOP HEALTH SERVICE CORPORATION | Grand Junction | E21Z Health Care | $42.2M | 9 |
| ASSOCIATION OF OPERATING ROOM NURSES | Denver | — | $42.1M | 12 |
| DENVER ART MUSEUM | Denver | A510 Arts, Culture & Humanities | $42M | 8 |
| DENVER BOTANIC GARDENS INC | Denver | C410 Environment | $41.6M | 7 |
| UNIVERSITY OF NORTHERN COLORADO | Greeley | B110 Education | $41.6M | 2 |
| CATHOLIC CHARITIES & COMMUNITY SERV | Denver | P200 Human Services | $41.3M | 6 |
| MERCY HOUSING CALIFORNIA | Denver | L20 Housing & Shelter | $41.1M | 14 |
| Air Force Academy Foundation | Usaf Academy | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $40.9M | 9 |
| ADOLPH COORS FOUNDATION | Denver | — | $40.8M | 1 |
| Arkansas Valley Regional Medical Center | La Junta | E220 Health Care | $39.2M | 8 |
| USA Swimming Inc | Colorado Springs | N67 Recreation & Sports | $38.5M | 12 |
| Grand Valley Rural Power Lines Inc | Grand Junction | — | $38.4M | 16 |
| MGMA-ACMPE | Englewood | S41 Community Improvement & Capacity Building | $38.3M | 11 |
| DEVELOPMENTAL DISABILITIES RESOURCE | Lakewood | P82 Human Services | $38.2M | 7 |
| HOSPICE OF METRO DENVER INC | Denver | P74Z Human Services | $38.2M | 11 |
| CLASSICAL ACADEMY | Colorado Springs | B99Z Education | $38.2M | 6 |
| Contractors Health Trust FKA Colorado | Englewood | — | $37.4M | 3 |
| AMERICAN WATER WORKS ASSOCIATION | Denver | W038 Public & Societal Benefit | $37.4M | 11 |
| CHERRY HILLS COUNTRY CLUB | Englewood | — | $37.3M | 8 |
| DEVELOPMENTAL DISABILITIES CENTER | Lafayette | P82Z Human Services | $37.3M | 3 |
| ROCKY MOUNTAIN PREPARATORY SCHOOLS | Denver | — | $37.1M | 9 |
| SOUTHEAST COLORADO POWER ASSOCIATION | La Junta | — | $36.9M | 15 |
| FIRST NATIONS DEVELOPMENT INSTITUTE | Longmont | — | $36.9M | 6 |
| COLORADO HEALTH NETWORK INC | Denver | G81Z Diseases, Disorders & Medical Disciplines | $36.5M | 6 |
| THE WILD ANIMAL SANCTUARY | Keenesburg | D340 Animal-Related | $36.4M | 5 |
| THE MYRON STRATTON HOME | Colorado Springs | — | $36.2M | 6 |
| BSLC II | Colorado Springs | P75 Human Services | $35.8M | 16 |
| COMMUNITY REACH CENTER INC | Westminster | E60 Health Care | $35.7M | 10 |
