| THE INTERNATIONAL COUNCIL ON CLEAN TRANSPORTATION INC | Washington | C052 Environment | $34.9M | 10 |
| CHIEF EXECUTIVES ORGANIZATION INC | Washington | S41 Community Improvement & Capacity Building | $34.9M | 11 |
| NATIONAL SOCIETY DAUGHTERS OF THE AMERICAN REVOLUTION | Washington | A800 Arts, Culture & Humanities | $34.8M | 8 |
| CRUISE LINES INTERNATIONAL ASSOCIATION INC | Washington | — | $34.7M | 11 |
| OCEANA INC | Washington | C013 Environment | $34.7M | 11 |
| SEARCH FOR COMMON GROUND | Washington | Q400 International, Foreign Affairs & National Security | $34.6M | 10 |
| RESOLVE UGANDA INCORPORATED | Washington | Q33 International, Foreign Affairs & National Security | $34.5M | 0 |
| TRANSPORT WORKERS UNION OF AMERICA | Washington | — | $34.4M | 25 |
| CAMPAIGN FOR TOBACCO-FREE KIDS | Washington | F520 Mental Health & Crisis Intervention | $34.3M | 14 |
| INSTITUTE OF INTERNATIONAL FINANCE | Washington | — | $34.2M | 14 |
| USAGING | Washington | — | $33.7M | 10 |
| Recording Industry Association Of America Inc | Washington | — | $33.6M | 11 |
| SOLAR ENERGY INDUSTRIES ASSOCIATION | Washington | — | $33.6M | 14 |
| COMMUNITY CONNECTIONS INC | Washington | F32Z Mental Health & Crisis Intervention | $33.6M | 9 |
| THE FAIRNESS PROJECT | Washington | L50 Housing & Shelter | $33.3M | 6 |
| CARLOS ROSARIO INTERNATIONAL PUBLIC CHARTER SCHOOL INC | Washington | B60 Education | $33.1M | 6 |
| PROTECT DEMOCRACY PROJECT | Washington | R05 Civil Rights, Social Action & Advocacy | $33.1M | 8 |
| Foundation for the Defense of Democracies Inc | Washington | Q01 International, Foreign Affairs & National Security | $32.5M | 11 |
| UNRWA USA NATIONAL COMMITTEE INC | Washington | Q33 International, Foreign Affairs & National Security | $32.4M | 4 |
| HOUSING COUNSELING SERVICES INC | Washington | L80Z Housing & Shelter | $32.4M | 7 |
| RESULTS FOR AMERICA | Washington | B80 Education | $32.3M | 8 |
| THE HOSPITAL FOR SICK CHILDREN | Washington | E240 Health Care | $32.1M | 11 |
| NATIONAL SPECTRUM CONSORTIUM INC | Washington | U01 Science & Technology | $31.9M | 1 |
| LABORERS' NATIONAL HEALTH & WELFARE FUND | Washington | — | $31.8M | 7 |
| JOURNALISM DEVELOPMENT NETWORK INC | Washington | B60 Education | $31.7M | 8 |
| MANAGED FUNDS ASSOCIATION | Washington | — | $31.7M | 11 |
| AMERICAN DENTAL EDUCATION ASSOCIATION | Washington | B50 Education | $31.7M | 14 |
| AMERICAN ASSOCIATION FOR JUSTICE | Washington | — | $31.7M | 10 |
| THE GERMAN MARSHALL FUND OF THE UNITED STATES | Washington | Q050 International, Foreign Affairs & National Security | $31.5M | 9 |
| NATIONAL ASS FOR EQUAL OPPORTUNITY IN HIGHER EDUCATION | Washington | — | $31.4M | 1 |
| CONGRESSIONAL BLACK CAUCUS FOUNDATION INC | Washington | R22Z Civil Rights, Social Action & Advocacy | $31.3M | 6 |
| URAC | Washington | S40Z Community Improvement & Capacity Building | $31.3M | 10 |
| American Battlefield Trust | Washington | A85Z Arts, Culture & Humanities | $31.2M | 9 |
| BIPARTISAN POLICY CENTER INC | Washington | W052 Public & Societal Benefit | $31.2M | 15 |
| THE WASHINGTON HOUSING CONSERVANCY INC | Washington | L20 Housing & Shelter | $30.8M | 0 |
| NATIONAL CHILDREN'S ALLIANCE INC | Washington | — | $30.5M | 8 |
| WOMEN FOR WOMEN INTERNATIONAL | Washington | Q300 International, Foreign Affairs & National Security | $30.5M | 12 |
| The Education Trust Inc | Washington | B90 Education | $30.4M | 15 |
| OPPORTUNITYWORK INC | Washington | J20 Employment | $30.4M | 14 |
| EARTHSCOPE CONSORTIUM | Washington | M05 Public Safety, Disaster Preparedness & Relief | $30.4M | 12 |
| ALLIANCE FOR AUTOMOTIVE INNOVATION INC | Washington | S41 Community Improvement & Capacity Building | $30.4M | 13 |
| IMPROVING ECONOMIES FOR STRONGER COMMUNITIES | Washington | — | $30.2M | 13 |
| NATIONAL AIR TRAFFIC CONTROLLERS ASSOCIATION AFL-CIO | Washington | — | $30M | 18 |
| Cotton Council International | Washington | — | $29.9M | 9 |
| RURAL COMMUNITY ASSISTANCE PARTNERSHIP INCORPORATED | Washington | — | $29.8M | 6 |
| WASHINGTON DRAMA SOCIETY INC | Washington | A650 Arts, Culture & Humanities | $29.8M | 9 |
| MUNDO VERDE BILINGUAL PUBLIC CHARTER SCHOOL | Washington | B29 Education | $29.7M | 6 |
| Public Welfare Foundation Inc | Washington | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $29.6M | 10 |
| NATIONAL CHILDREN'S CENTER INC | Washington | P8XZ Human Services | $29.6M | 3 |
| American College of Cardiology | Washington | E03 Health Care | $29.5M | 28 |
