| TREZEVANT EPISCOPAL HOME | Memphis | P750 Human Services | $38M | 7 |
| MARTHA O'BRYAN CENTER INC | Nashville | X20 Religion-Related | $37.8M | 4 |
| UNIVERSITY SCHOOL OF NASHVILLE | Nashville | B430 Education | $37.4M | 9 |
| THE HARPETH HALL SCHOOL | Nashville | B200 Education | $37.2M | 8 |
| FEDERAL EXPRESS CORPORATION WELFARE BENEFITS TRUST | Collierville | — | $37.1M | 1 |
| Credit Unions chartered in the State of Tennessee | Nashville | — | $36.7M | 12 |
| VALOR COLLEGIATE ACADEMIES | Nashville | B29 Education | $36.6M | 7 |
| Claiborne Medical Center | Knoxville | E20 Health Care | $36.6M | 10 |
| BEN LOMAND RURAL TELEPHONE COOPERATIVE | McMinnville | — | $36.6M | 18 |
| MEMPHIS UNIVERSITY SCHOOL | Memphis | B250 Education | $36.4M | 8 |
| NASHVILLE ZOO INC | Nashville | D500 Animal-Related | $35.9M | 11 |
| DETTWILLER FOUNDATION | Nashville | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $35.9M | 3 |
| Southern College of Optometry | Memphis | B500 Education | $35.6M | 7 |
| BRENTWOOD ACADEMY | Brentwood | B24Z Education | $35.5M | 7 |
| UNITED FOOD & COMMERICAL WORKERS EMPL HEALTH & WELFARE | Cordova | — | $35.5M | 0 |
| FREEDOM PREPARATORY ACADEMY INC | Memphis | B24 Education | $35.4M | 7 |
| BAPTIST MEMORIAL HOSPITAL- HUNTINGDON INC | Huntingdon | E220 Health Care | $34.7M | 11 |
| SOUTHEASTERN IRON WORKERS WELFARE PLAN | Goodlettsville | — | $34.3M | 0 |
| OPERATION SHARING TENNESSEE | Oneida | P122 Human Services | $33.9M | 0 |
| WEBB SCHOOL OF KNOXVILLE | Knoxville | B200 Education | $33.7M | 12 |
| BRIARCREST CHRISTIAN SCHOOL SYSTEM INC | Eads | B200 Education | $33.4M | 7 |
| Fort Sanders Perinatal Center | Knoxville | — | $33.3M | 9 |
| ONE GENERATION AWAY | Franklin | R12 Civil Rights, Social Action & Advocacy | $32.5M | 1 |
| SEBRING SENIOR LIVING INC | Cleveland | — | $32.4M | 2 |
| Nashville Symphony Association | Nashville | A690 Arts, Culture & Humanities | $32M | 12 |
| GIRLS PREPARATORY SCHOOL INC | Chattanooga | B200 Education | $31.7M | 6 |
| SOUTHEAST FINANCIAL CREDIT UNION C/O ADAM TOUNGETTE | Franklin | — | $31.6M | 10 |
| Tennessee Aquarium | Chattanooga | D500 Animal-Related | $31.5M | 10 |
| JR HYDE III FAMILY FOUNDATION | Memphis | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $31.5M | 3 |
| Le Bonheur Children's Hospital Foundation | Memphis | E112 Health Care | $31.4M | 12 |
| Porter-Leath | Memphis | — | $31.3M | 7 |
| Shelby Residential & Vocational Services Inc | Memphis | P820 Human Services | $31.3M | 8 |
| Abahac Inc | Brentwood | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $31.1M | 0 |
| CURREY INGRAM ACADEMY | Brentwood | B28Z Education | $31M | 6 |
| SOUTHEAST LABORERS HEALTH FUND | Goodlettsville | — | $30.9M | 0 |
| LAUSANNE COLLEGIATE SCHOOL | Memphis | B21Z Education | $30.8M | 7 |
| PHILANTHROPIC IMPACT FOUNDATION | Franklin | — | $30.5M | 0 |
| BAPTIST MEMORIAL COLLEGE OF HEALTH SCIENCES INC | Memphis | B500 Education | $30.2M | 16 |
| VULTEE CHARITABLE FOUNDATION F/K/A NASHVILLE CHRISTIAN TOWERS | Nashville | — | $29.8M | 1 |
| SECOND HARVEST FOOD BANK OF NORTHEAST TN | Kingsport | K310 Food, Agriculture & Nutrition | $29.3M | 1 |
| BATTLE GROUND ACADEMY | Franklin | B25Z Education | $29.2M | 7 |
| CHATTANOOGA CHRISTIAN SCHOOL INC | Chattanooga | B200 Education | $29M | 6 |
| BILL AND CAROL LATIMER CHARITABLE FOUNDATION | Union City | B82 Education | $29M | 0 |
| MEMPHIS CITY EMPLOYEES CREDIT UNION | Memphis | — | $29M | 8 |
| Bledsoe Telephone Cooperative Inc | Pikeville | — | $28.8M | 15 |
| ST MARY'S EPISCOPAL SCHOOL | Memphis | — | $28.6M | 7 |
| MIDDLE TENNESSEE STATE UNIVERSITY FOUNDATION | Murfreesboro | B110 Education | $28.4M | 0 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF METROPOLITAN CHATTANOOGA | Chattanooga | — | $28.4M | 8 |
| DOUGLAS CHEROKEE ECONOMIC AUTHORITY INC | Morristown | B22Z Education | $28.3M | 2 |
| MUELLER INDUSTRIES INC BENEFITS TRUST | Collierville | — | $28.2M | 2 |
