| LOURDES UNIVERSITY | Sylvania | B420 Education | $35M | 10 |
| HENRY COUNTY HOSPITAL INC | Napoleon | E22Z Health Care | $34.9M | 9 |
| EDISON WELDING INSTITUTE | Columbus | J220 Employment | $34.9M | 14 |
| PELOTONIA | Columbuus | B11 Education | $34.7M | 7 |
| MARYHAVEN INC | Columbus | F22Z Mental Health & Crisis Intervention | $34.7M | 12 |
| NORTH CENTRAL ELECTRIC COOPERATIVE INC | Attica | — | $34.5M | 19 |
| Columbus Jewish Foundation | Columbus | T70 Philanthropy, Voluntarism & Grantmaking Foundations | $34.5M | 7 |
| UNION BENEFITS TRUST | Westerville | — | $34.4M | 0 |
| Cincinnati Hills Christian Academy | Cincinnati | B200 Education | $34.3M | 7 |
| THE CORPORATION FOR OHIO APPALACHIAN DEVELOPMENT | Athens | S200 Community Improvement & Capacity Building | $34.2M | 1 |
| Bluffton University | Bluffton | B420 Education | $33.9M | 5 |
| BAYLEY SENIOR CARE | Cincinnati | P750 Human Services | $33.9M | 7 |
| Jewish Federation of Cincinnati | Cincinnati | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $33.9M | 9 |
| HOSPICE OF NORTHWEST OHIO | Perrysburg | — | $33.8M | 8 |
| Greenacres Foundation Inc | Cincinnati | C32 Environment | $33.8M | 3 |
| ASHTABULA COUNTY MEDICAL CENTER GROUP RETURN | Ashtabula | — | $33.8M | 9 |
| THE FOODBANK INC | Dayton | K31 Food, Agriculture & Nutrition | $33.7M | 3 |
| WOOD COUNTY SCHOOLS BENEFIT PLAN | Perrysburg | — | $33.7M | 0 |
| WILLOW BROOK CHRISTIAN SERVICES | Delaware | L22 Housing & Shelter | $33.6M | 7 |
| KETTERING ADVENTIST HEALTHCARE | Miamisburg | — | $33.6M | 13 |
| TWIN TOWERS | Cincinnati | P750 Human Services | $33.5M | 4 |
| HOLZER MEDICAL CENTER - JACKSON | Gallipolis | E220 Health Care | $33.5M | 10 |
| UNIVERSITY OF RIO GRANDE | Rio Grande | B430 Education | $33.5M | 10 |
| PREVAILING WAGE CONTRACTORS ASSOCIATION TRUST | Columbus | — | $33.4M | 1 |
| ARC INDUSTRIES INC OF FRANKLIN COUNTY OHIO | Columbus | — | $33.3M | 9 |
| NORTHWESTERN OHIO PLUMBERS & PIPEFITTERS HEALTH & WELFARE PLAN & TRUST | Northwood | — | $33.2M | 0 |
| NESTLE IN THE USA RETIREE WELFARE BENEFIT TRUST | Solon | — | $33.2M | 1 |
| CINCINNATI MUSEUM CENTER | Cincinnati | A500 Arts, Culture & Humanities | $33.2M | 7 |
| JOHN J & MARY R SCHIFF FOUNDATION | Cincinnati | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $33.1M | 3 |
| ST LEONARD | Centerville | L22 Housing & Shelter | $33M | 8 |
| COLUMBUS NEIGHBORHOOD HEALTH CENTER INC | Columbus | E32 Health Care | $32.6M | 10 |
| PROMEDICA FOUNDATION | Toledo | W11 Public & Societal Benefit | $32.4M | 6 |
| LIMA MEMORIAL PROFESSIONAL CORPORATION | Lima | E30 Health Care | $32.3M | 18 |
| JUDSON | Cleveland | P750 Human Services | $32.2M | 11 |
| Ohio Shared Information Services | Cincinnati | T50 Philanthropy, Voluntarism & Grantmaking Foundations | $32.1M | 10 |
| FIRST COMMUNITY VILLAGE | Columbus | L220 Housing & Shelter | $32M | 9 |
| DILEY RIDGE MEDICAL CENTER | Columbus | E20 Health Care | $32M | 13 |
| AKRON CHILDREN'S HOSPITAL FOUNDATION | Akron | E110 Health Care | $32M | 7 |
| TOURNAMENTS FOR CHARITY C/O JOHN JANKOVIC | Dublin | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $31.9M | 0 |
| OUCU FINANCIAL CREDIT UNION FKA OHIO UNIVERSITY CREDIT UNION | Athens | — | $31.9M | 13 |
| Hospice of the Western Reserve at University Hospitals LLC | Cleveland | — | $31.9M | 6 |
| SUNSET RETIREMENT COMMUNITIES INC | Toledo | P750 Human Services | $31.6M | 10 |
| HAMILTON COMMUNITY FOUNDATION | Hamilton | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $31.6M | 3 |
| YOUNG MENS CHRISTIAN ASSOCIATION OF CLEVELAND | Cleveland | P270 Human Services | $31.3M | 7 |
| THE NATIONAL REGISTRY OF EMERGENCY MEDICAL TECHNICIANS | Columbus | M194 Public Safety, Disaster Preparedness & Relief | $31.3M | 10 |
| HOSPICE OF CENTRAL OHIO | Newark | — | $31.2M | 10 |
| THE FINDLAY-HANCOCK COUNTY COMMUNITY FOUNDATION | Findlay | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $31M | 3 |
| SCHOTTENSTEIN STORES CORPORATION ASSOCIATES' BENEFIT PLAN SS2 | Columbus | — | $30.9M | 1 |
| CINCINNATI COUNTRY DAY SCHOOL | Cincinnati | B200 Education | $30.9M | 8 |
| HEBREW ACADEMY OF CLEVELAND | Cleveland Heights | — | $30.6M | 5 |
