| NORTHEAST OHIO NEIGHBORHOOD HEALTH | Cleveland | — | $28M | 8 |
| FIRST COMMUNITY VILLAGE | Columbus | L220 Housing & Shelter | $28M | 11 |
| SUNSHINE INC RESIDENTIAL & SUPPORT | Maumee | P730 Human Services | $27.9M | 8 |
| CROSSROADS HEALTH | Mentor | F30Z Mental Health & Crisis Intervention | $27.9M | 10 |
| THE NATIONAL REGISTRY OF EMERGENCY | Columbus | M194 Public Safety, Disaster Preparedness & Relief | $27.9M | 11 |
| BRETHREN RETIREMENT COMMUNITY | Greenville | P750 Human Services | $27.8M | 9 |
| ReliabilityFirst Corporation | Cleveland | W80 Public & Societal Benefit | $27.8M | 22 |
| THE JEWISH FOUNDATION OF CINCINNATI | Cincinnati | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $27.8M | 1 |
| CARROLL ELECTRIC COOPERATIVE INC | Carrollton | — | $27.7M | 15 |
| DEFIANCE COLLEGE | Defiance | B420 Education | $27.7M | 4 |
| COMMUNITY ACTION AGENCY OF COLUMBIANA | Lisbon | P20Z Human Services | $27.6M | 7 |
| Knox Energy Cooperative | Canton | Y22 Mutual & Membership Benefit | $27.6M | 5 |
| COLLEGE NOW GREATER CLEVELAND INC | Cleveland | B820 Education | $27.6M | 10 |
| JEWISH FAMILY SERVICE ASSOCIATION OF CLEVELAND OHIO | Pepper Pike | X30 Religion-Related | $27.4M | 8 |
| MIAMI UNIVERSITY FOUNDATION | Oxford | B110 Education | $27.3M | 12 |
| THE GOODWILL INDUSTRIES OF AKRON | Akron | — | $27.2M | 7 |
| ST LEONARD | Centerville | L22 Housing & Shelter | $27.1M | 7 |
| MID-OHIO ENERGY COOPERATIVE INC | Kenton | — | $27.1M | 18 |
| AHF OHIO INC | Dublin | E99 Health Care | $27M | 8 |
| Ohio Children's Alliance | Columbus | — | $27M | 5 |
| CINCINNATI MUSEUM CENTER | Cincinnati | A500 Arts, Culture & Humanities | $26.9M | 7 |
| KIPP COLUMBUS | Columbus | B25 Education | $26.9M | 6 |
| COLUMBUS URBAN LEAGUE | Columbus | — | $26.8M | 8 |
| SPECIALIZED ALTERNATIVES FOR | Delphos | P320 Human Services | $26.8M | 6 |
| THE TOLEDO MUSEUM OF ART | Toledo | A510 Arts, Culture & Humanities | $26.8M | 8 |
| COLUMBUS HOUSING PARTNERSHIP INC | Columbus | L200 Housing & Shelter | $26.8M | 7 |
| DELTA ZETA NATIONAL HOUSING CORPORATION | Oxford | — | $26.8M | 1 |
| THE SUSANNE GEIER PETERS FOUNDATION | Loveland | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $26.7M | 0 |
| Barnesville Hospital Association Inc | Barnesville | E220 Health Care | $26.7M | 11 |
| LIFECARE HOSPICE | Wooster | — | $26.7M | 10 |
| COOPER HATCHERY INC | Fort Recovery | — | $26.6M | 0 |
| GOVERNMENT EMPLOYEES | Cleveland | — | $26.6M | 0 |
| TOLEDO COMMUNITY FOUNDATION INC | Toledo | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $26.6M | 4 |
| The Greater Dayton School | Miamisburg | B20 Education | $26.4M | 4 |
| UNIVERSAL 1 CREDIT UNION | Dayton | — | $26.4M | 12 |
| COMMQUEST SERVICES INC | Canton | P462 Human Services | $26.4M | 7 |
| INTERNATIONAL UNION OF OPERATING | Cleveland | — | $26.3M | 36 |
| IMPACT COMMUNITY ACTION INC | Columbus | S20 Community Improvement & Capacity Building | $26.2M | 3 |
| OHIO'S HOSPICE INC | Dayton | E19 Health Care | $26.1M | 18 |
| BELLEVUE GARDENS COMMUNITY URBAN | Cincinnati | — | $26.1M | 0 |
| LAUREL SCHOOL | Shaker Heights | B200 Education | $26M | 5 |
| GREATER COLUMBUS ARTS COUNCIL | Columbus | A260 Arts, Culture & Humanities | $26M | 5 |
| DAYTON FOUNDATION PLUS INC | Dayton | T11 Philanthropy, Voluntarism & Grantmaking Foundations | $25.9M | 7 |
| GATEWAYS TO BETTER LIVING INC | Youngstown | P73Z Human Services | $25.8M | 2 |
| ARC INDUSTRIES INC OF FRANKLIN COUNTY | Columbus | — | $25.7M | 10 |
| FRANKLIN COUNTY HISTORICAL SOCIETY | Columbus | A80 Arts, Culture & Humanities | $25.4M | 8 |
| SJO KIDS INC | Cincinnati | P30 Human Services | $25.4M | 5 |
| GREENFIELD AREA MEDICAL CENTER | Greenfield | — | $25.4M | 11 |
| COMMUNITY HOSPITALS AND WELLNESS CENTERS | Bryan | E220 Health Care | $25.3M | 17 |
| DAYTON FOUNDATION DEPOSITORY | Dayton | T31Z Philanthropy, Voluntarism & Grantmaking Foundations | $25.3M | 7 |
