| CINCINNATI-HAMILTON COUNTY COMMUNITY ACTION AGENCY | Cincinnati | P200 Human Services | $39.4M | 5 |
| THE OHIO MASONIC HOME THE OHIO MASONIC HOME AFFILIATES | Springfield | L23Z Housing & Shelter | $39.4M | 11 |
| COLONIAL SENIOR SERVICES | Hamilton | L22 Housing & Shelter | $39.4M | 8 |
| FAIRFIELD HEALTHCARE PROFESSIONALS | Lancaster | — | $39.3M | 7 |
| LIFEWISE INC | Hilliard | O50 Youth Development | $39.3M | 4 |
| NOTRE DAME COLLEGE | South Euclid | B420 Education | $39.2M | 3 |
| LIFEBANC | Cleveland | — | $39.2M | 7 |
| YOUNG MEN'S CHRISTIAN ASSOCIATION OF GREATER DAYTON | Dayton | — | $39.2M | 8 |
| WOOSTER COMMUNITY HOSPITAL FOUNDATION | Wooster | E19 Health Care | $39.2M | 8 |
| CRAIG AND FRANCES LINDNER CENTER OF HOPE | Mason | F31 Mental Health & Crisis Intervention | $39.2M | 11 |
| COLUMBUS CENTER FOR HUMAN SERVICES INC | Columbus | P730 Human Services | $39.1M | 5 |
| MID-AMERICAN ATHLETIC CONFERENCE INC | Cleveland | — | $39M | 5 |
| UNION CONSTRUCTION WORKERS HEALTH PLAN | Holland | — | $38.9M | 1 |
| HATHAWAY BROWN SCHOOL | Shaker Heights | B200 Education | $38.8M | 7 |
| YOUNGSTOWN STATE UNIVERSITY FOUNDATION | Youngstown | B11 Education | $38.8M | 4 |
| STARK COMMUNITY FOUNDATION | Canton | T310 Philanthropy, Voluntarism & Grantmaking Foundations | $38.5M | 8 |
| UNITED CHURCH INSURANCE COMPANY | Cleveland | Y20 Mutual & Membership Benefit | $38.5M | 4 |
| Lifeline of Ohio Organ Procurement Inc | Columbus | E65Z Health Care | $38.4M | 10 |
| COMMUNITY ACTION COMMITTEE OF PIKE COUNTY INC | Piketon | P200 Human Services | $38.3M | 7 |
| BUTLER RURAL ELECTRIC COOP INC | Oxford | — | $38.3M | 16 |
| PIPEFITTERS UNION HEALTH & WELFARE FUND LOCAL 392 | Cincinnati | Y43 Mutual & Membership Benefit | $38.3M | 3 |
| POSITIVE EDUCATION PROGRAM | Cleveland | F330 Mental Health & Crisis Intervention | $38.3M | 12 |
| TWIN LAKES | Cincinnati | L22 Housing & Shelter | $38.2M | 4 |
| THE JOURNEY FOUNDATION | Columbus | B12 Education | $38.1M | 0 |
| Circle Health Services | Cleveland | — | $38.1M | 7 |
| THE CLEVELAND INSTITUTE OF ART | Cleveland | B420 Education | $37.9M | 9 |
| Citizens Not Politicians | Columbus | — | $37.9M | 0 |
| DEACONESS ASSOCIATIONS INC | Cincinnati | E110 Health Care | $37.9M | 2 |
| INTERNATIONAL BROTHERHOOD OF ELECTRICAL WORKERS LOCAL 38 HEALTH & WELFARE F | Cleveland | — | $37.8M | 0 |
| Wilmington College | Wilmington | B420 Education | $37.8M | 9 |
| PLANNED PARENTHOOD OF GREATER OHIO | Akron | E42 Health Care | $37.7M | 9 |
| SOUTHWEST GENERAL MEDICAL GROUP INC | Middleburg Heights | E30Z Health Care | $37.7M | 10 |
| LIGHTHOUSE YOUTH SERVICES | Cincinnati | P300 Human Services | $37.4M | 7 |
| OHIO BANKERS BENEFITS TRUST | Columbus | — | $37.2M | 1 |
| CLEVELAND INSTITUTE OF MUSIC | Cleveland | B500 Education | $37M | 11 |
| CINCINNATI NATURE CENTER | Milford | C600 Environment | $36.9M | 5 |
| Trust Agreement for the Ohio Farmers Insurance Co Group Health Benefit Plan | Westfield Center | Y34 Mutual & Membership Benefit | $36.9M | 0 |
| BLANCHARD VALLEY CONTINUING CARE SERVICES | Findlay | L22 Housing & Shelter | $36.7M | 9 |
| THE SEVEN HILLS SCHOOL | Cincinnati | B250 Education | $36.5M | 11 |
| COLUMBUS SYMPHONY ORCHESTRA | Columbus | A690 Arts, Culture & Humanities | $36.3M | 0 |
| Strategies to End Homelessness Inc | Cincinnati | P85 Human Services | $36.3M | 5 |
| Cleveland State University Foundation | Cleveland | B43I Education | $36.2M | 1 |
| LUTHERAN HOMES SOCIETY INC | Toledo | P40 Human Services | $35.7M | 9 |
| COMMUNICARE HEALTH BENEFIT TRUST | Cincinnati | Y43 Mutual & Membership Benefit | $35.7M | 0 |
| BETHESDA FOUNDATION INC | Cincinnati | E112 Health Care | $35.6M | 5 |
| OHIO CAPITAL CORPORATION FOR HOUSING | Columbus | L190 Housing & Shelter | $35.5M | 24 |
| THE UNIVERSITY OF TOLEDO FOUNDATION | Toledo | B110 Education | $35.4M | 8 |
| NATIONAL FOOTBALL MUSEUM INC | Canton | A500 Arts, Culture & Humanities | $35.3M | 10 |
| COLUMBUS ASSOCIATION FOR THE PERFORMING ARTS | Columbus | A610 Arts, Culture & Humanities | $35.3M | 8 |
| PIKE HEALTH SERVICES INC | Waverly | E99Z Health Care | $35.1M | 10 |
