| UNITED FAITH MINISTRIES INC | Norfolk | X20 Religion-Related | $49.3M | 7 |
| THE PROTESTANT EPISCOPAL HIGH SCHOOL | Alexandria | B250 Education | $49.3M | 7 |
| OLD DOMINION LAND CONSERVANCY INC | Purcellville | B99 Education | $48.7M | 1 |
| NORTHERN NECK ELECTRIC COOPERATIVE | Warsaw | — | $48.2M | 14 |
| WORLWIDE ASSURANCE FOR EMPLOYEES OF | Falls Church | Y44 Mutual & Membership Benefit | $47.9M | 8 |
| FS-ISAC INC | Reston | S41 Community Improvement & Capacity Building | $47.4M | 14 |
| THE STUDENT CONSERVATION ASSOCIATION | Arlington | — | $47.3M | 11 |
| NATIONAL ASSOCIATION OF CORPORATE | Arlington | W03 Public & Societal Benefit | $47M | 12 |
| LAKEWOOD MANOR BAPTIST RETIREMENT | Glen Allen | P750 Human Services | $46.9M | 12 |
| THE COLLEGE FOUNDATION OF THE UNIVERSITY | Charlottesville | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $46.9M | 8 |
| CHRISTIAN RELIEF SERVICES INC | Alexandria | Q330 International, Foreign Affairs & National Security | $46.8M | 5 |
| WILLIAMSBURG LANDING INC | Williamsburg | P750 Human Services | $46.7M | 7 |
| PIEDMONT ACCESS TO HEALTH SERVICES | Danville | E30 Health Care | $46.6M | 11 |
| WOODBERRY FOREST SCHOOL | Woodberry Forest | B250 Education | $46.2M | 10 |
| EASTERN SHORE RURAL HEALTH | Onancock | E39Z Health Care | $45.8M | 14 |
| Children's Hospital and Healthcare Services Foundation | Richmond | E11 Health Care | $45.5M | 7 |
| RAINFOREST TRUST | Warrenton | Q300 International, Foreign Affairs & National Security | $45.5M | 5 |
| Leadership Institute | Arlington | W700 Public & Societal Benefit | $45M | 15 |
| CLAWS FOUNDATION | Reston | T20 Philanthropy, Voluntarism & Grantmaking Foundations | $44.8M | 0 |
| INTERNET SOCIETY | Reston | Q300 International, Foreign Affairs & National Security | $44.7M | 9 |
| The Catholic Charities USA Employee | Alexandria | Y20 Mutual & Membership Benefit | $44.6M | 0 |
| NEIGHBORHOOD HEALTH | Alexandria | E32 Health Care | $44.3M | 6 |
| WESTMINSTER-CANTERBURY OF THE BLUE RIDGE | Charlottesville | P750 Human Services | $44.1M | 8 |
| THE GOOD FOOD INSTITUTE INC | Arlington | K03 Food, Agriculture & Nutrition | $43.6M | 8 |
| AMERICA'S CHARITIES | Chantilly | — | $43.6M | 6 |
| Sweet Briar Institute | Sweet Briar | B420 Education | $43.6M | 9 |
| FERRUM COLLEGE | Ferrum | B420 Education | $43.5M | 7 |
| Beth Sholom Home of Eastern Virginia | Virginia Beach | P750 Human Services | $43.4M | 6 |
| Riverside Healthcare Association Inc | Newport News | E21 Health Care | $43.4M | 9 |
| VIRGINIA PRIVATE COLLEGES | Bedford | Y43 Mutual & Membership Benefit | $42.8M | 2 |
| INTERNATIONAL ASSOCIATION OF CHIEFS OF | Alexandria | — | $42.7M | 13 |
| UNIVERSITY OF MARY WASHINGTON FOUNDATION | Fredericksburg | B42 Education | $42.6M | 3 |
| Carilion Clinic | Roanoke | E192 Health Care | $42.5M | 28 |
| MATTRESS RECYCLING COUNCIL INC | Alexandria | C27 Environment | $42.2M | 2 |
| ASHOKA | Arlington | Q300 International, Foreign Affairs & National Security | $42.1M | 9 |
| FEEDING SOUTHWEST VIRGINIA | Salem | K31 Food, Agriculture & Nutrition | $42.1M | 6 |
| Community Residences INC | Chantilly | P73Z Human Services | $42.1M | 6 |
| AIR-CONDITIONING HEATING AND | Arlington | S41 Community Improvement & Capacity Building | $42.1M | 14 |
| MILITARY BENEFIT ASSOCIATION | Chantilly | — | $42M | 7 |
| William & Mary Foundation | Williamsburg | B11 Education | $41.1M | 7 |
| INTERNATIONAL WATER MANAGEMENT INSTITUTE | Alexandria | K22Z Food, Agriculture & Nutrition | $40.9M | 19 |
| World Help | Forest | X99Z Religion-Related | $40.8M | 6 |
| UNITED METHODIST FAMILY SERVICES OF | Richmond | P73Z Human Services | $40.7M | 7 |
| NORTHERN VIRGINIA FAMILY SERVICE INC | Falls Church | P46Z Human Services | $40.6M | 10 |
| VALLEYSTAR CREDIT UNION | Martinsville | — | $40.6M | 34 |
| UNIVERSITY OF VIRGINIA FOUNDATION | Charlottesville | B110 Education | $40.4M | 12 |
| National Assn of Chain Drug Stores Inc | Arlington | — | $40.3M | 12 |
| NATIONAL INDUSTRIES FOR THE BLIND | Alexandria | — | $40M | 13 |
| CLOSE UP FOUNDATION | Arlington | A30 Arts, Culture & Humanities | $39.9M | 12 |
| INTERNET SOCIETY FOUNDATION | Reston | Q11 International, Foreign Affairs & National Security | $39.8M | 10 |
