| GRADUATE MANAGEMENT ADMISSION COUNCIL | Reston | B90 Education | $63.7M | 14 |
| AMERICAN INSTITUTE IN TAIWAN | Arlington | Q99Z International, Foreign Affairs & National Security | $63.6M | 10 |
| JOE DACY CHARITABLE TRUST | Reston | — | $63.3M | 0 |
| UVA PRINCE WILLIAM HEALTH SYSTEM | Manassas | — | $63.3M | 13 |
| ENTREPRENEURS ORGANIZATION | Alexandria | B036 Education | $62.7M | 13 |
| HOLLINS UNIVERSITY CORPORATION | Roanoke | B430 Education | $62.7M | 8 |
| OLD DOMINION UNIVERSITY RESEARCH | Norfolk | B110 Education | $62.4M | 12 |
| NATIONAL ASSOCIATION OF CONVENIENCE | Alexandria | — | $61.8M | 15 |
| BAY AGING | Urbanna | P81Z Human Services | $61.6M | 6 |
| THE COMMON APPLICATION INC | Arlington | B90 Education | $61.3M | 11 |
| SUNNYSIDE PRESBYTERIAN HOME | Harrisonburg | P750 Human Services | $61M | 9 |
| VIRGINIA BANKERS ASSOCIATION MEDICAL AND | Glen Allen | — | $60.9M | 1 |
| THE POTOMAC SCHOOL | McLean | B200 Education | $60.3M | 7 |
| MARY BALDWIN UNIVERSITY | Staunton | B42Z Education | $60.2M | 7 |
| CONNECTED GIVING FOUNDATION | Reston | U41 Science & Technology | $59.7M | 3 |
| National Milk Producers Federation | Arlington | — | $59.5M | 9 |
| Blumont Global Development Inc | Arlington | Q33 International, Foreign Affairs & National Security | $59.3M | 10 |
| ANDECO INSTITUTE | McLean | W20 Public & Societal Benefit | $59.1M | 10 |
| SOUTHERN ENVIRONMENTAL LAW CENTER | Charlottesville | — | $58.2M | 19 |
| THE PAULEY FAMILY FOUNDATION | Richmond | T30 Philanthropy, Voluntarism & Grantmaking Foundations | $57.9M | 0 |
| NATIONAL APARTMENT ASSOCIATION | Arlington | — | $57.7M | 14 |
| The Railroad Employees National Early | Arlington | — | $57.7M | 2 |
| Young Men's Christian Association of South Hampton Roads | Chesapeake | P270 Human Services | $57.6M | 13 |
| Young Men's Christian Association of Greater Richmond (6769) | Richmond | P270 Human Services | $57.4M | 13 |
| LUTHERAN FAMILY SERVICES OF VIRGINIA INC | Roanoke | P400 Human Services | $56.4M | 11 |
| FOODBANK OF SOUTHEASTERN VIRGINIA | Norfolk | K310 Food, Agriculture & Nutrition | $56.1M | 4 |
| UNIVERSITY OF VIRGINIA ALUMNI | Charlottesville | B840 Education | $55.8M | 12 |
| MODZELEWSKI CHARITABLE TR US 100196 | Reston | T20Z Philanthropy, Voluntarism & Grantmaking Foundations | $55.5M | 0 |
| THE COUNTRY CLUB OF VIRGINIA INC | Richmond | — | $54.7M | 13 |
| EASTERN MENNONITE UNIVERSITY | Harrisonburg | — | $54.7M | 6 |
| AVERETT UNIVERSITY | Danville | B430 Education | $54.7M | 8 |
| PRINCE GEORGE ELECTRIC COOPERATIVE | Waverly | — | $54.4M | 17 |
| BEDFORD MEMORIAL HOSPITAL | Bedford | E220 Health Care | $53.3M | 10 |
| Medical College of VA Foundation | Richmond | B11 Education | $53.2M | 7 |
| AMERICAN SOCIETY OF CIVIL ENGINEERS | Reston | U030 Science & Technology | $53M | 10 |
| The Concord Fund | Vienna | — | $52.8M | 3 |
| Graham Holdings Company EBAT | Arlington | — | $52.3M | 1 |
| NBA PLAYERS' HEALTH AND WELFARE BENEFIT | North Chesterfield | — | $51.6M | 0 |
| AMERICAN SOCIETY FOR TRAINING AND | Alexandria | J030 Employment | $51.5M | 7 |
| NATIONAL RURAL LETTER CARRIERS' | Alexandria | — | $51.4M | 17 |
| AMERICAN PHYSICAL THERAPY ASSOCIATION | Alexandria | E03 Health Care | $51.3M | 29 |
| UNIVERSITY OF VIRGINIA LAW SCHOOL | Charlottesville | B110 Education | $51.1M | 9 |
| VIRGINIA UNION UNIVERSITY | Richmond | B430 Education | $51M | 9 |
| AMERICAN ASSOCIATION OF MOTOR VEHICLE | Arlington | W20C Public & Societal Benefit | $51M | 11 |
| ARMY NAVY COUNTRY CLUB | Arlington | — | $50.7M | 15 |
| VIRGINIA STUDENT AID FOUNDATION INC | Charlottesville | B820 Education | $50.3M | 8 |
| INSTITUTE FOR JUSTICE | Arlington | R600 Civil Rights, Social Action & Advocacy | $49.9M | 11 |
| YOUTH FOR TOMORROW-NEW LIFE CENTER INC | Bristow | O55Z Youth Development | $49.7M | 10 |
| Flint Hill School | Oakton | B200 Education | $49.4M | 9 |
| ROBIN MERGER CORPORATION INC | Arlington | B030 Education | $49.4M | 10 |
