| GIVE BACK FOUNDATION | Madison | T12 Philanthropy, Voluntarism & Grantmaking Foundations | $69.7M | 1 |
| Prairie du Chien Memorial Hospital | Prairie Du Chien | E22Z Health Care | $69M | 8 |
| WAUPUN MEMORIAL HOSPITAL | Waupun | E220 Health Care | $68.7M | 9 |
| LUTHERAN SOCIAL SERVICES OF WISCONSIN | West Allis | P200 Human Services | $68.3M | 13 |
| WISCONSIN EVANGELICAL LUTHERAN SYNOD | Wauwatosa | — | $68.1M | 0 |
| THE LAKES COMMUNITY HEALTH CENTER INC | Iron River | — | $67.9M | 9 |
| SIXTEENTH STREET COMMUNITY HEALTH | Milwaukee | — | $66.8M | 7 |
| CHILDREN'S SERVICE SOCIETY OF WISCONSIN | Milwaukee | — | $66.5M | 15 |
| Rock Energy Cooperative | Janesville | — | $64.9M | 16 |
| BETTERLIFE | Madison | — | $64.3M | 25 |
| VITERBO UNIVERSITY | La Crosse | B420 Education | $64.2M | 8 |
| UNITED WAY OF GREATER MILWAUKEE & | Milwaukee | T700 Philanthropy, Voluntarism & Grantmaking Foundations | $64.2M | 7 |
| ALFRED AND ISABEL BADER 2014 CHARITABLE TRUST | Milwaukee | T22 Philanthropy, Voluntarism & Grantmaking Foundations | $63.7M | 0 |
| COMMUNITY FOUNDATION FOR THE FOX VALLEY | Appleton | T31 Philanthropy, Voluntarism & Grantmaking Foundations | $60.6M | 6 |
| LADD MEMORIAL HOSPITAL INC | Osceola | E220 Health Care | $59.3M | 12 |
| EDGEWOOD COLLEGE INC | Madison | B430 Education | $59M | 10 |
| ICS SUPPORT ORGANIZATION INC | Franklin | B11 Education | $58.3M | 5 |
| ThedaCare Medical Center - Waupaca Inc | Appleton | E22 Health Care | $56.4M | 10 |
| THEDACARE MEDICAL CENTER - BERLIN INC | Appleton | E220 Health Care | $56M | 12 |
| BADER PHILANTHROPIES INC | Milwaukee | — | $55.9M | 1 |
| USONA INSTITUTE INC | Madison | H99 Medical Research | $54.9M | 0 |
| UNITED COMMUNITY CENTER INC | Milwaukee | P840 Human Services | $53.3M | 8 |
| WISCONSIN SHEET METAL WORKERS HEALTH | Waukesha | — | $53.1M | 7 |
| FAMILY HEALTH CENTER OF MARSHFIELD INC | Marshfield | E30Z Health Care | $52.4M | 9 |
| WESTBY CO-OP CREDIT UNION | Westby | — | $52.2M | 14 |
| SECOND HARVEST FOODBANK OF SOUTHERN WI | Madison | K31Z Food, Agriculture & Nutrition | $51.9M | 4 |
| WISCONSIN LUTHERAN COLLEGE INC | Milwaukee | — | $51.3M | 8 |
| GRANT REGIONAL HEALTH CENTER INC | Lancaster | E320 Health Care | $51.2M | 9 |
| ASSOCIATED BANC-CORP GROUP HEALTH | Green Bay | — | $51.2M | 0 |
| EXPERIMENTAL AIRCRAFT ASSOCIATION INC | Oshkosh | U030 Science & Technology | $50.8M | 12 |
| WISCONSIN COMMUNITY SERVICES INC | Milwaukee | I40Z Crime & Legal-Related | $49.9M | 7 |
| NORTH CENTRAL STATES REGIONAL COUNCIL OF | St Paul | S41 Community Improvement & Capacity Building | $49.7M | 18 |
| SUPERIOR CHOICE CREDIT UNION | Superior | — | $49.3M | 19 |
| St Joseph's Hospital of the Hospital Sisters of the Third Order of St Franc | Chippewa Falls | E220 Health Care | $47.5M | 10 |
| GREATER WISCONSIN AGENCY ON AGING | Madison | — | $47.2M | 1 |
| RIVERLAND ENERGY COOPERATIVE | Arcadia | — | $46.5M | 15 |
| OAKDALE ELECTRIC COOPERATIVE | Oakdale | — | $45.9M | 14 |
| SLIPSTREAM GROUP INC | Madison | C350 Environment | $45.8M | 8 |
| ACCESS COMMUNITY HEALTH CENTERS INC | Madison | — | $45.6M | 20 |
| HORIZON HOME CARE & HOSPICE INC | Milwaukee | E99Z Health Care | $45.5M | 9 |
| BLACKHAWK COMMUNITY CREDIT UNION | Janesville | — | $44.6M | 14 |
| ST JOSEPH'S HEALTH SERVICES INC | Hillsboro | E22 Health Care | $44.6M | 16 |
| QUARTZ HEALTH PLAN MN CORPORATION | Fitchburg | E80 Health Care | $44.3M | 6 |
| LAKELAND UNIVERSITY | Plymouth | B420 Education | $43.5M | 12 |
| BARRON ELECTRIC COOPERATIVE | Barron | — | $42.9M | 15 |
| GOODWILL INDUSTRIES OF SOUTH CENTRAL | Madison | J32Z Employment | $42.8M | 5 |
| FRIENDS OF BE AN ANGEL INC | Mequon | Q71 International, Foreign Affairs & National Security | $42.7M | 1 |
| Milwaukee Center For Independence Inc | Milwaukee | P8XZ Human Services | $42.4M | 9 |
| ALVERNO COLLEGE | Milwaukee | B420 Education | $41.6M | 13 |
| WORKMONEY INC | Milwaukee | — | $41.4M | 9 |
